| Budget Amount *help |
¥24,310,000 (Direct Cost: ¥18,700,000、Indirect Cost: ¥5,610,000)
Fiscal Year 2014: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2013: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Fiscal Year 2012: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
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| Outline of Final Research Achievements |
The clue to establish a new modified method to purify pure MSCs is to know the expression pattern of surface markers on not only heterogeneous MSCs, but also committed cells, such as osteoblasts, adipocytes, and chondrocytes. But there is one obstacle to know the expression patterns of surface markers, that is mineral deposits produced by osteoblasts during culturing period. We developed a new method, which separates differentiated cells from mineral deposits using Percoll gradients. This new technique is a helpful tool to identify MSC surface markers. On the other hand, we analyzed LIF function on osteoblast differentiation. SOCS3 expression was induced by LIF/STAT3 signaling. SOCS3 knockdown demonstrated that shSOCS3-BMSCs almost completely abrogated the LIF suppressive effect. Our results demonstrated that LIF suppresses osteoblast differentiation through the LIF/STAT3/SOCS3 signaling pathway.
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