Establishing dental epithelial cell lines for in vitro tooth enamel bioengineering
| Project/Area Number |
24689073
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | The Nippon Dental University |
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Principal Investigator |
NAKAHARA TAKA 日本歯科大学, 生命歯学部, 教授 (10366768)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥26,130,000 (Direct Cost: ¥20,100,000、Indirect Cost: ¥6,030,000)
Fiscal Year 2014: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
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| Keywords | 細胞・組織 / 細胞培養 / 器官培養 / 分化誘導 / エナメル形成 / 移植・再生医療 / 再生医学 / 歯学 |
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| Outline of Final Research Achievements |
Bioengineering of tooth enamel is an important goal in regenerative dentistry. We aimed to establish ameloblastic cell lines using primary culture of dental tissues or pluripotent stem cells such as embryonic stem (ES) cells. Here, we describe “cell fishing”, a novel, filter paper-based, cell-manipulation technique to isolate cells from a heterogeneous cell culture population. Based on their morphologically distinct appearance, epithelial cells were selectively isolated from rat periodontal ligament-derived and porcine enamel epithelium-derived tissue cultures. Dental epithelial cell lines were established from these cultured cells. Attempts are ongoing to establish an epithelial cell line derived from ES cells via the formation of embryoid body and its prolonged cultivation . These dental cell lines could serve as competent cell sources to bioengineer tooth enamel in vitro.
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Report
(4 results)
Research Products
(133 results)
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[Presentation] ヒト乳歯の歯髄に万能細胞は存在するか？2014
Author(s)
石川 博, 大山晃弘, 高橋 悠, 川上未有希, 鈴木見奈子, 田中 彰, 中原 貴
Organizer
第59回社団法人日本口腔外科学会総会・学術大会
Place of Presentation
幕張メッセ（千葉県・千葉市）
Year and Date
2014-10-17 – 2014-10-19
Related Report
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[Presentation] ヒトの歯髄細胞に万能細胞は存在するか？2014
Author(s)
大山晃弘, 豊村順子, 高橋 悠, 川上未有希, 鈴木見奈子, 中原 貴, 田中 彰, 石川 博
Organizer
平成26年度日本歯科大学歯学会大会・総会
Place of Presentation
日本歯科大学生命歯学部展示ホール（東京都・千代田区）
Year and Date
2014-06-07
Related Report
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[Presentation] ヒト脂肪組織由来幹細胞の骨細胞への分化と骨形成2012
Author(s)
大山晃弘,井出吉昭, Tansriratanawong Kallapat, 田巻友一,富永徳子,中原貴,立花利公,渡邊美隆,栗原邦弘,佐藤聡,石川博
Organizer
平成24年度日本歯科大学歯学会大会
Place of Presentation
日本歯科大学生命歯学部メモリアルホール,東京
Year and Date
2012-06-02
Related Report
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[Presentation] インプラントと再生医療
Author(s)
中原 貴
Organizer
平成25年度日本歯科大学歯学会大会・総会
Place of Presentation
日本歯科大学新潟生命歯学部講堂, 新潟
Related Report
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