| Budget Amount *help |
¥26,130,000 (Direct Cost: ¥20,100,000、Indirect Cost: ¥6,030,000)
Fiscal Year 2014: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
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| Outline of Final Research Achievements |
Bioengineering of tooth enamel is an important goal in regenerative dentistry. We aimed to establish ameloblastic cell lines using primary culture of dental tissues or pluripotent stem cells such as embryonic stem (ES) cells. Here, we describe “cell fishing”, a novel, filter paper-based, cell-manipulation technique to isolate cells from a heterogeneous cell culture population. Based on their morphologically distinct appearance, epithelial cells were selectively isolated from rat periodontal ligament-derived and porcine enamel epithelium-derived tissue cultures. Dental epithelial cell lines were established from these cultured cells. Attempts are ongoing to establish an epithelial cell line derived from ES cells via the formation of embryoid body and its prolonged cultivation . These dental cell lines could serve as competent cell sources to bioengineer tooth enamel in vitro.
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