Analysis of the mechanism of Nfil3-mediated suppression of neurodegeneration

• Project/Area Number: 24700314
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neuroscience in general
• Research Institution: The University of Tokyo
• Principal Investigator: KURABAYASHI Nobuhiro 東京大学, 理学(系)研究科(研究院), 助教 (40581658)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 神経変性 / 筋萎縮性側索硬化症 / Nfil3 / 筋萎縮性側索硬化症（ALS） / 神経保護 / ALS

Outline of Final Research Achievements

Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by the loss of motor neurons. In this study, by using an ALS mouse model, we found that transcription factor Nfil3 is a neuroprotective molecule intrinsic to the neuronal defense mechanism. In addition, we searched for the molecule(s) which may acts downstream of Nfil3 and affects neuroprotective systems in cell-based ALS model. We identified a novel molecule which may constitute a cell defense mechanism in neurons.

Research Products

• [Journal Article] In vivo role of phosphorylation of cryptochrome 2 in the mouse circadian clock. (2014)
  • Author(s): Hirano A, Kurabayashi N, Nakagawa T, Shioi G, Todo T, Hirota T, Fukada Y.
  • Journal Title: Mol. Cell. Biol. Volume: 34(24) Issue: 24 Pages: 4464-73
  • DOI: 10.1128/mcb.00711-14
  • Peer Reviewed / Open Access
• [Journal Article] Neuroprotective role of the basic leucine zipper transcription factor NFIL3 in models of amyotrophic lateral sclerosis (2014)
  • Author(s): So-ichi Tamai, Keisuke Imaizumi, Nobuhiro Kurabayashi, Minh Dang Nguyen, Takaya Abe, Masatoshi Inoue, Yoshitaka Fukada & Kamon Sanada
  • Journal Title: J Biol Chem Volume: 289 Issue: 3 Pages: 1629-38
  • DOI: 10.1074/jbc.m113.524389
  • Peer Reviewed / Open Access
• [Journal Article] Increased dosage of DYRK1A and DSCR1 delays neuronal differentiation in neocortical progenitor cells (2013)
  • Author(s): Kurabayashi Nobuhiro
  • Journal Title: Genes and Development Volume: 27 Issue: 24 Pages: 2708-2721
  • DOI: 10.1101/gad.226381.113
  • Peer Reviewed
• [Journal Article] The G protein-coupled receptor GPRC5B contributes to neurogenesis in the developing mouse neocortex. (2013)
  • Author(s): Nobuhiro Kurabayashi
  • Journal Title: Development Volume: 140 Issue: 21 Pages: 4335-4346
  • DOI: 10.1242/dev.099754
  • Peer Reviewed
• [Journal Article] Increased anxiety in offspring reared by circadian Clock mutant mice (2013)
  • Author(s): Hiroko Koizumi, Nobuhiro Kurabayashi, Yuto Watanabe & Kamon Sanada
  • Journal Title: PLoS One Volume: 12 Issue: 6 Pages: e66021-e66021
  • DOI: 10.1371/journal.pone.0066021
  • Peer Reviewed
• [Presentation] ダウン症関連因子DYRK1AとDSCR1の過剰発現による神経幹細胞の神経分化抑制 (2014)
  • Author(s): 倉林伸博
  • Organizer: 第37回日本分子生物学会年回
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] 大脳新皮質神経前駆細胞の運命決定における7TMの役割 (2014)
  • Author(s): 武尾優
  • Organizer: 第37回日本分子生物学会年回
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] bZIP型転写因子Nfil3による筋萎縮性側索硬化症モデルマウスの神経変性の抑止 (2014)
  • Author(s): 今泉景介
  • Organizer: 第37回日本分子生物学会年回
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] 筋萎縮性側索硬化症モデルにおけるNfil3の神経保護作用 (2012)
  • Author(s): 倉林伸博
  • Organizer: 第８５回 日本生化学会大会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡
• [Remarks] 眞田研究室ホームページ
  • URL: http://park.itc.u-tokyo.ac.jp/mgrl/sanada/index.html
• [Remarks] 眞田研究室
  • URL: http://park.itc.u-tokyo.ac.jp/mgrl/sanada/index.html