| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
|---|
| Outline of Final Research Achievements |
We found that the stress-regulated protein M6a is one of the most abundant membrane protein in the axonal growth cone during the neuronal development, and it regulates the extracellular matrix-dependent determination of neuronal polarity through its interaction with intra-, extracellular signal transduction molecules. M6a was expressed in the cortices as well as hippocampi, and regulates the neuronal polarity determination in both neurons.
shRNA knockdown experiment of M6a in the embryonic mice brain showed that acute suppression of M6a in the cerebral cortex delayed morphological transition of migrating cortical neurons from multipolar to mono- (bi-) polar in the intermediate zone and their axon formation.
Our data suggest that epigenetic depression of M6a delay the determination of neuronal polarity essential for neuronal network in the brain.
|
