| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
The function of the hippocampal CA2 region remains largely unknown. The aim of this study was to examine the role of CA2 neurons in memory by conditional targeting. To develop a CA2 neuron-selective gene targeting system, we made several knock-in lines, in which Cre recombinase or tetracycline transactivator was inserted into the translational initiation site of the target genes (Cacng5, Rgs14, Abat and so on) in frame. The genes seem to be strongly expressed in the CA2 region. Additionally, we made a new line expressing diphteria toxin receptor under control of the activity of Cre recombinase or the induction of tetracycline transactivator. The mutant lines developed in this study could be useful tools for functional analysis of hippocampus-dependent memory.
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