Analysis of the axon collateral branch formation mechanism using an inducible gene expression technique in corticospinal tract and pontine nuclei

• Project/Area Number: 24700323
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neuroscience in general
• Research Institution: Osaka University (2013); University of Fukui (2012)
• Principal Investigator: IGUCHI Tokuichi 大阪大学, 医学(系)研究科(研究院), 助教 (60509305)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 神経科学 / 解剖学 / 発生・分化 / 神経回路 / 軸索側枝

Research Abstract

Corticospinal tract is a major subcortical output from the neocortex. During the development of corticospinal tract, axon collaterals protrude from their main shaft toward the various nuclei. Axon collaterals are thought to have an important role in the coordination of neural activities. However, molecular mechanisms by which axon collaterals are induced are mostly unknown. To obtain the molecules responsible for the induction of axon collaterals, we performed microarray analyses on the target of axon collaterals, and got some candidates. We also obtained the candidates of the receptor that responds to the signals from the target of axon collaterals. Knockdown of some candidate receptors in the corticospinal neurons showed inhibition or promotion of axon collateralization. To analyze the candidate molecules, we developed the analysis method enabling us manipulation of the gene expression and observation of the microtubule dynamics in the corticospinal tract and the pons.

Research Products

• [Journal Article] Smoothened controls cyclin D2 expression and regulates the generation of intermediate progenitors in the developing cortex (2013)
  • Author(s): Komada, M., Iguchi, T., Takeda, T., Ishibashi, M., Sato, M.
  • Journal Title: Neurosci Lett Volume: 547 Pages: 87-91
  • Peer Reviewed
• [Journal Article] WAVE2-Abi2 complex controls growth cone activity and regulates the multipolar-bipolar transition as well as the initiation of glia-guided migration (2013)
  • Author(s): Xie, MJ., Yagi, H., Kuroda, K., Wang, CC., Komada, M., Zhao, H., Sakakibara, A., Miyata, T., Nagata, K., Oka, Y., Iguchi, T., Sato, M.
  • Journal Title: Cereb Cortex Volume: 23(6) Pages: 1410-23
  • Peer Reviewed
• [Journal Article] Smoothened controls cyclin D2 expression and regulates the generation of intermediate progenitors in the developing cortex. (2013)
  • Author(s): Komada M, Iguchi T, Takeda T, Ishibashi M, Sato M.
  • Journal Title: Neuroscience Letters Volume: 547 Pages: 87-91
  • DOI: 10.1016/j.neulet.2013.05.006
  • Peer Reviewed
• [Journal Article] WAVE2-Abi2 complex controls growth cone activity and regulates the multipolar-bipolar transition as well as the initiation of glia-guided migration (2013)
  • Author(s): Xie, M.-J., Yagi, H., Kuroda, K., Wang C.-C., Komada, M., Zhao, H., Sakakibara, A., Miyata, T., Nagata, K., Oka, Y., Iguchi, T. and Sato, M.
  • Journal Title: Cerebral Cortex Volume: (in press) Issue: 6 Pages: 1410-1423
  • DOI: 10.1093/cercor/bhs123
  • Peer Reviewed
• [Presentation] Search and analysis of the candidate molecules involved in axon collateralization in the corticospinal tract (2013)
  • Author(s): Iguchi T, Omi M, Oka Y, Sato M.
  • Organizer: Neuro2013
• [Presentation] Search and analysis of the candidate molecules involved in axon collateralization in the corticospinal tract (2013)
  • Author(s): Tokuichi Iguchi, Minoru Omi, Yuichiro Oka, Makoto Sato
  • Organizer: Neuro2013
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] Analyses on the potential candidates of collateral branch inducing factor that are specifically expressed in the targets of axon collaterals from the corticospinal projection (2012)
  • Author(s): Iguchi T, Oka Y, Kuroda K, Wang CC, Xie MJ, Yagi H, Sato M.
  • Organizer: JSN-APSN 2012神戸
  • Place of Presentation: 神戸
• [Presentation] Search and analysis of the candidate molecules involved in axon collateralization in the corticospinal tract
  • Author(s): Iguchi T, Omi M, Oka Y, Sato M
  • Organizer: Neuro2013
  • Place of Presentation: 国立京都国際会館（京都府）
• [Presentation] Analyses on the potential candidates of collateral branch inducing factor that are specifically expressed in the targets of axon collaterals from the corticospinal projection.
  • Author(s): Iguchi T, Oka Y, Kuroda K, Wang CC, Xie MJ, Yagi H, Sato M.
  • Organizer: JSN-APSN 2012
  • Place of Presentation: 神戸国際会議場（兵庫県）
• [Presentation] 皮質脊髄路の発達における軸索側枝形成因子の探索
  • Author(s): 猪口 徳一
  • Organizer: ORIGIN神経科学研究会夏のワークショップ
  • Place of Presentation: 石川県小松市
• [Book] 脳科学辞典編集委員会「翼板」脳科学辞典 (2013)
  • Author(s): 猪口徳一、佐藤真
  • URL: http://bsd.neuroinf.jp/wiki/
• [Book] 「翼板」 脳科学辞典 http://bsd.neuroinf.jp/wiki/翼板 (2013)
  • Author(s): 猪口徳一 佐藤真
  • Total Pages: 1
  • Publisher: 脳科学辞典編集委員会
• [Remarks]
  • URL: http://www.anat2.med.osaka-u.ac.jp/index.html