Identification and functional analysis of transcription factors regulating Musashi1 in neural stem/progenitor cells

• Project/Area Number: 24700334
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neuroscience in general
• Research Institution: Keio University
• Principal Investigator: KAWASE Satoshi 慶應義塾大学, 医学部, 特任助教 (80468496)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 神経発生 / 神経幹細胞 / 転写制御 / エンハンサー / Musashi1 / Rfx / 転写因子 / Msi1 / 国際情報交換 / USA

Research Abstract

Musashi1 is specifically expressed in neural stem/progenitor cells and involved in their maintenance and proliferation. Because Musashi1 also plays a role in glioma cell growth, the analysis of transcriptional regulation of Musashi1 gene is important, however, the regulatory factor has been unclear. In this study, we showed that the transcription factor Rfx4 promote the transcription of Musashi1 gene. Our results suggest that the ciliogenic Rfx4 transcription factor regulates the maintenance of neural stem/progenitor cell.

Research Products

• [Presentation] Identification of a novel intronic enhancer responsible for the transcriptional regulation of Musashi1 in neural stem/progenitor cells (2012)
  • Author(s): Satoshi Kawase
  • Organizer: Society for Neuroscience annual meeting 2012 (Sfn)
  • Place of Presentation: ニューオーリンズ、アメリカ合衆国
  • Year and Date: 2012-10-15
• [Presentation] Identification of a novel intronic enhancer responsible for the transcriptional regulation of Musashi1 in neural stem/progenitor cells (2012)
  • Author(s): Satoshi Kawase
  • Organizer: International Society for Stem Cell Research 10^<th> annual meeting (ISSCR)
  • Place of Presentation: 横浜
  • Year and Date: 2012-06-13
• [Presentation] Identification of a novel intronic enhancer responsible for the transcriptional regulation of Musashi1 in neural stem/progenitor cells
  • Author(s): Satoshi Kawase, Takao Imai, Chikako Miyauchi-Hara, Yoshinori Nishimoto, Shin-ichi Fukami, Kunio Yaguchi, Shigeyoshi Itohara, Yumi Matsuzaki1, Atsushi Miyawaki, Hideyuki Okano
  • Organizer: International Society for Stem Cell Research 10th annual meeting
  • Place of Presentation: Yokohama
• [Presentation] Identification of a Novel Intronic Enhancer Responsible for the Transcriptional Regulation of Musashi1 in Neural Stem/Progenitor cells
  • Author(s): Satoshi Kawase, Takao Imai, Kunio Yaguchi, Shigeyoshi Itohara, Hideyuki Okano
  • Organizer: Society for Neuroscience annual meeting 2012
  • Place of Presentation: New Orleans