Research Project
Grant-in-Aid for Young Scientists (B)
In this study, we identified the genes specifically expressed in the long association neurons connecting distant cortical areas in order to examine the detailed axon projection patterns and developmental processes of the circuit. First, we identified the genes expressed in the long association neurons by microarray analysis comparing gene expression profiles of the long association neurons and the callosal neurons, followed by double staining analysis combining retrograde tracing and in situ hybridization. We then used the promoters of these genes to drive the expression of the fluorescent reporter. We visualized the long association neurons and their axons by introducing the reporter constructs into the embryonic cortex and are currently analyzing the development of the neural circuit by examining the axonal patterns at different stages. For efficient observation and analysis, we also set up the tissue clearing procedure for the cortex using SeeDB method.
All 2014 2013 2012 Other
All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (12 results) (of which Invited: 1 results) Book (2 results) Remarks (1 results)
Sci Rep
Volume: 4 Issue: 1 Pages: 4037-4037
10.1038/srep04037
Cerebral Cortex
Volume: (in press) Issue: 6 Pages: 1410-1423
10.1093/cercor/bhs123
Volume: 3 Issue: 1 Pages: 2063-2063
10.1038/srep02063
SNJ1945 Scientific Reports
Volume: 3 Issue: 1 Pages: 1224-1224
10.1038/srep01224
http://bsd.neuroinf.jp/wiki/
http://www.anat2.med.osaka-u.ac.jp/index.html
