Analysis on the long association fibers in the cerebral cortex using a specific promoter
| Project/Area Number |
24700352
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Osaka University (2013)
University of Fukui (2012) |
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Principal Investigator |
OKA Yuichiro 大阪大学, 医学(系)研究科(研究院), 助教 (30614432)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 神経回路網 / 分子神経生物学 / 神経科学 / 神経解剖学 / 大脳皮質 / 長連合線維 / 領野間回路 / in utero electroporation |
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| Research Abstract |
In this study, we identified the genes specifically expressed in the long association neurons connecting distant cortical areas in order to examine the detailed axon projection patterns and developmental processes of the circuit. First, we identified the genes expressed in the long association neurons by microarray analysis comparing gene expression profiles of the long association neurons and the callosal neurons, followed by double staining analysis combining retrograde tracing and in situ hybridization. We then used the promoters of these genes to drive the expression of the fluorescent reporter. We visualized the long association neurons and their axons by introducing the reporter constructs into the embryonic cortex and are currently analyzing the development of the neural circuit by examining the axonal patterns at different stages. For efficient observation and analysis, we also set up the tissue clearing procedure for the cortex using SeeDB method.
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Report
(3 results)
Research Products
(19 results)
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[Journal Article] WAVE2-Abi2 complex controls growth cone activity and regulates the multipolar-bipolar transition as well as the initiation of glia-guided migration2013
Author(s)
Xie, M.-J., Yagi, H., Kuroda, K., Wang C.-C., Komada, M., Zhao, H., Sakakibara, A., Miyata, T., Nagata, K., Oka, Y., Iguchi, T. and Sato, M.
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Journal Title
Cerebral Cortex
Volume: (in press)
Issue: 6
Pages: 1410-1423
DOI
Related Report
Peer Reviewed
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[Journal Article] Post-natal therapeutic intervention for lissencephaly using a blood-brain-barrier permeable calpain inhibitor2013
Author(s)
Shiori Toba, Yasuhisa Tamura, Kanako Kumamoto, Masami Yamada, Keizo Takao, Satoko Hattori, Tsuyoshi Miyakawa, Yosky Kataoka, Mitsuyoshi Azuma, Kiyoshi Hayasaka, Masano Amamoto, Keiko Tominaga, Anthony Wynshaw-Boris, Mitsuhiro Kato and Shinji Hirotsune
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Journal Title
SNJ1945 Scientific Reports
Volume: 3
Issue: 1
Pages: 1224-1224
DOI
Related Report
Peer Reviewed
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