p73 signaling regulates mice depressive behavior thorough control of adult neurogenesis.

• Project/Area Number: 24700355
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: Osaka University
• Principal Investigator: FUJITANI Masashi 大阪大学, 連合小児発達学研究科, 助教 (40376372)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: p73 / 神経幹細胞 / 神経新生 / p53 / 抑うつ

Research Abstract

In order to establish animal model of depression, low dose glucocorticoid was administered to wild type mice as previous report, but it failed. Then we established p73 knock-in and knockout mouse, and confirmed p73 has no expression in adult hippocampus. Even with restraint, p73 expression is not changed in hippocampus. However we have finally found that p73 regulates ependymal cell cycle. Brain injury induces TAp73 expression and it decreases ependymal cell proliferation via unknown humoral signaling.

Research Products

• [Journal Article] Transient maternal IL- 6 mediates long- lasting changes in neural stem cell pools by deregulating an endogenous self- renewal pathway (2013)
  • Author(s): Gallagher D, Norman AA, Woodard CL, Yang G, Gauthier- Fisher A, Fujitani M, Vessey JP,Cancino GI, Sachewski N, Woltjen K, Fatt MP, Morshead CM,Kaplan DR, Miller FD.
  • Journal Title: Cell stem cell 2013 Volume: 13(5) Pages: 564-576
  • Peer Reviewed
• [Journal Article] A proapoptotic effect of valproic acid on progenitors of embryonic stem cell derived glutamatergic neurons (2013)
  • Author(s): Fujiki, R., Sato, A., Fujitani, M. and Yamashita, T.
  • Journal Title: Cell death dis 2013 Volume: 20(4)
  • Peer Reviewed
• [Journal Article] Transient maternal IL-6 mediates long-lasting changes in neural stem cell pools by deregulating an endogenous self-renewal pathway. (2013)
  • Author(s): Gallagher D, Norman AA, Woodard CL, Yang G, Gauthier-Fisher A, Fujitani M, Vessey JP,Cancino GI, Sachewski N, Woltjen K, Fatt MP, Morshead CM,Kaplan DR, Miller FD
  • Journal Title: Cell stem cell Volume: 13(5) Issue: 5 Pages: 564-576
  • DOI: 10.1016/j.stem.2013.10.002
  • Peer Reviewed
• [Journal Article] A proapoptotic effect of valproic acid on progenitors of embryonic stem cell-derived glutamatergic neurons (2013)
  • Author(s): Fujiki, R., Sato, A., Fujitani, M. and Yamashita, T
  • Journal Title: Cell Death and Disease Volume: 20(4) Issue: 6 Pages: e677-e677
  • DOI: 10.1038/cddis.2013.205
  • Peer Reviewed
• [Journal Article] Improvement in protocol to generate homogeneous glutamatergic neurons from mouse embryonic stem cells reduced apoptosis. (2013)
  • Author(s): Fujiki, R., et al.
  • Journal Title: Biochem Biophys Res Commun. Volume: 430 Issue: 2 Pages: 604-609
  • DOI: 10.1016/j.bbrc.2012.11.106
  • Peer Reviewed
• [Presentation] 16番染色体関連miR484はPCDH19を介して神経幹細胞の分化を制御する (2014)
  • Author(s): 藤谷昌司
  • Organizer: 第七回神経発生討論会
  • Place of Presentation: 大阪大学
  • Year and Date: 2014-03-13
• [Presentation] microRNA- 484 located in chromosome 16p13.11 locus, controls cortical development (2014)
  • Author(s): 藤谷昌司
  • Organizer: International Symposiumon Glyco- Neuroscience
  • Place of Presentation: 淡路夢舞台
  • Year and Date: 2014-01-09
• [Presentation] microRNA-484 located in chromosome 16p13.11 locus, controls cortical development. (2014)
  • Author(s): 藤谷昌司
  • Organizer: International Symposium on Glyco-Neuroscience
  • Place of Presentation: 淡路夢舞台
• [Presentation] 16 番染色体関連 miR484 は PCDH19 を介して神経幹細胞の分化を制御する (2014)
  • Author(s): 藤谷昌司
  • Organizer: 神経発生討論会
  • Place of Presentation: 大阪大学
• [Presentation] microRNA- 484は皮質神経前駆細胞の分化を制御する (2013)
  • Author(s): 藤谷昌司
  • Organizer: 第14回ORIGIN神経科学研究会
  • Place of Presentation: 下呂温泉
  • Year and Date: 2013-08-31
• [Presentation] 16番染色体16p13に着目した新規脳発生関連分子の発見と小児神経疾患モデル動物作成の試み (2013)
  • Author(s): 藤谷昌司
  • Organizer: 第二回大阪大学神経難病フォーラム
  • Place of Presentation: 大阪大学
  • Year and Date: 2013-08-03
• [Presentation] microRNA-484は皮質神経前駆細胞の分化を制御する (2013)
  • Author(s): 藤谷昌司
  • Organizer: 第14回 ORIGIN 神経科学研究会
  • Place of Presentation: 下呂温泉
• [Presentation] miR-484は神経幹細胞の分化・維持を制御する (2013)
  • Author(s): Masashi Fujitani,ZHANG, SUXIANG,Toshihide Yamashita
  • Organizer: 平成24年度 CREST領域ミーティング
  • Place of Presentation: ベルサール神保町
• [Presentation] p73は、神経幹細胞の維持、及び神経細胞の 生存維持に必須である (2013)
  • Author(s): 藤谷昌司
  • Organizer: 新学術領域 神経糖鎖生物学 冬の班会議
  • Place of Presentation: 宮崎
• [Presentation] 視神経の軸索再生阻害の分子機構とその制御による再生の誘導 (2013)
  • Author(s): 藤谷昌司
  • Organizer: NewYearOphthalmic Seminar 2013 in 東海
  • Place of Presentation: 名古屋
  • Invited
• [Presentation] MINP REGULATES RADIAL MIGRATION RATE IN THE DEVELOPING NEOCORTEX (2012)
  • Author(s): ZHANG, SUXIANG Masashi Fujitani Toshihide Yamashita
  • Organizer: ISSCR(第10回国際幹細胞学会）
  • Place of Presentation: パシフィコ横浜
• [Book] 疾患モデルの作成と利用― がん 第 8 章 脳神経系 第3節 神経芽腫 (2012)
  • Author(s): 藤谷昌司、 原口清輝、 中川原章
  • Total Pages: 8
  • Publisher: LEC