Analysis of cholinergic neurons derived from Alzheimer's disease patient-specific iPS cells

• Project/Area Number: 24700367
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: Fujita Health University (2013); Kyoto University (2012)
• Principal Investigator: YAHATA Naoki 藤田保健衛生大学, 医学部, 助教 (60450607)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: アルツハイマー病 / iPS細胞 / 分化誘導 / 神経細胞

Research Abstract

We have generated disease-specific iPS cells from familial Alzheimer's disease patients with Presenilin 1 mutations (AD-iPSCs). AD or control iPSCs were differentiated into cortical neurons mainly containing glutamatergic neurons. The production ratio of the pathogenic amyloid beta species was increased in AD-neurons. Microarray analysis of purified neurons revealed distinct gene expression profiles between AD and control neurons.

Research Products

• [Journal Article] A drug screening platform for Alzheimer's disease with intracellular Aβ oligomers using patient-specific iPSCs (2013)
  • Author(s): Kondo, T., (他24名), Murakami, K., Irie, K., Klein, W. L., Mori, H., Asada, T., Takahashi, R., Iwata, N., Yamanaka, S., Inoue, H
  • Journal Title: Cell Stem Cell Volume: 12 Issue: 4 Pages: 487-496
  • DOI: 10.1016/j.stem.2013.01.009
  • Peer Reviewed / Open Access
• [Journal Article] iPS細胞作製技術を利用した神経疾患病因機構の解明と創薬開発への取り組み (2012)
  • Author(s): 八幡直樹、井上治久
  • Journal Title: 遺伝子医学MOOK Volume: 22 Pages: 98-102
• [Presentation] 神経分化過程におけるAβ40とAβ42の産生比の変化とその責任遺伝子の解析 (2013)
  • Author(s): 浅井将、中野梨絵、荒木希、渡邊かおり、八幡直樹、関恒慶、小林千浩、戸田達史、城谷圭朗、井上治久、岩田修永
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2013-09-13
• [Presentation] 家族性アルツハイマー病患者由来iPS細胞の樹立と解析 (2013)
  • Author(s): 近藤孝之、八幡直樹、舟山美里、月田香代子、浅井将、岩田修永、川勝忍、和泉唯信、梶龍兒、朝田隆、高橋良輔、井上治久
  • Organizer: 第54回日本神経学会総会
  • Place of Presentation: 東京国際フォーラム(東京)
  • Year and Date: 2013-05-30
• [Presentation] Anti-Amyloidβdrug validation using human iPS cell-derived neurons for the treatment of Alzheimer's disease (2013)
  • Author(s): Asai M, Yahata N, Iwata N, and Inoue H.
  • Organizer: The 11^<th> International Conference on Alzheimer's & Parkinson's Disease
  • Place of Presentation: Firenze Fiera (Florence, Italy)
  • Year and Date: 2013-03-07
• [Presentation] 家族性アルツハイマー病患者由来iPS細胞の樹立と解析 (2013)
  • Author(s): 近藤孝之、八幡直樹、舟山美里、月田香代子、浅井将、岩田修永、川勝忍、和泉唯信、梶龍兒、朝田隆、高橋良輔、井上治久
  • Organizer: 第54回日本神経学会総会
  • Place of Presentation: 東京国際フォーラム
• [Presentation] 神経分化過程におけるAβ40とAβ42の産生比の変化とその責任遺伝子の解析 (2013)
  • Author(s): 浅井 将、中野 梨絵、荒木 希、渡邊 かおり、八幡 直樹、関 恒慶、小林 千浩、戸田 達史、城谷 圭朗、井上 治久、岩田 修永
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Anti-Amyloidβdrug validation using human iPS cell-derived neurons for the treatment of Alzheimer's disease (2012)
  • Author(s): Yahata N, Asai M, Iwata N, and Inoue H.
  • Organizer: The 42th Annual meeting of the Society for Neuroscience
  • Place of Presentation: the Ernest N. Morial Convention Center (New Orleans, LA)
  • Year and Date: 2012-10-17
• [Presentation] Anti-Aβdrug screening platform using human iPS cell-derived neurons for the treatment of Alzheimer's disease (2012)
  • Author(s): Yahata N, Asai M, Iwata N, and Inoue H.
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場(愛知)
  • Year and Date: 2012-09-18
• [Presentation] ヒトiPS細胞を用いたアルツハイマー病治療薬探索基盤の開発 (2012)
  • Author(s): 八幡直樹、浅井将、岩田修永、井上治久
  • Organizer: 第53回日本神経学会総会
  • Place of Presentation: 東京国際フォーラム(東京)
  • Year and Date: 2012-05-22
• [Presentation] ヒトiPS細胞を用いたアルツハイマー病治療薬探索基盤の開発
  • Author(s): 八幡直樹、浅井 将、岩田 修永、井上 治久
  • Organizer: 第53回日本神経学会総会
  • Place of Presentation: 東京
• [Presentation] Anti-Aβ drug screening platform using human iPS cell-derived neurons for the treatment of Alzheimer's disease.
  • Author(s): Yahata N., Asai M., Iwata N., and Inoue H.
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 愛知
• [Presentation] Anti-Amyloid β drug validation using human iPS cell-derived neurons for the treatment of Alzheimer's disease.
  • Author(s): Yahata N., Asai M., Iwata N., and Inoue H.
  • Organizer: The 42th Annual meeting of the Society for Neuroscience
  • Place of Presentation: New Orleans, USA
• [Presentation] Anti-Aβ drug validation using human iPS cell-derived neurons for the treatment of Alzheimer’s disease
  • Author(s): Asai M., Yahata N., Iwata N., and Inoue H.
  • Organizer: The 11th International Conference on Alzheimer's & Parkinson's Diseases
  • Place of Presentation: Florence, Italy
• [Book] iPS細胞作製技術を利用した神経疾患病因機構の解明と創薬開発への取り組み 遺伝子医学MOOK 22 (2012)
  • Author(s): 八幡直樹、井上治久
• [Remarks] 京都大学 iPS細胞研究所
  • URL: http://www.cira.kyoto-u.ac.jp/j/index.html
• [Remarks] 京都大学 iPS細胞研究所 臨床応用研究部門 井上治久研究室
  • URL: http://www.cira.kyoto-u.ac.jp/inoue/index.php
• [Remarks] 研究成果：ヒトiPS細胞を用いたアルツハイマー病治療薬探索基盤の開発
  • URL: http://www.cira.kyoto-u.ac.jp/j/research/finding/111025-113843.html
• [Remarks] 研究成果：患者さん由来iPS細胞でアルツハイマー病の病態を解明
  • URL: http://www.cira.kyoto-u.ac.jp/j/research/finding/130222-085354.html