| Project/Area Number |
24700369
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Kansai Medical University |
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Principal Investigator |
WATE REIKA 関西医科大学, 医学部, 講師 (50440988)
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| Research Collaborator |
OKI Mitsuaki 関西医科大学, 医学部
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 神経変性疾患 / 質量顕微鏡 / 神経病理 |
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| Outline of Final Research Achievements |
Neurodegenerative diseases are increasingly being realized to have common cellular and molecular mechanisms including protein aggregation and inclusion body formation, however the pathomechanisms and proteins underlying the inclusions are not completely understood. A new method for regional quantitation of mRNA at the basal ganglia of model rats by real time RT-PCR showed the change of the quontity of mRNA. The analysis of the disease-related proteins with matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) technology showed some unique protein in the spinal cord of ALS patients in comparison with disease control. Theu are alpha-internexin, anexin A5, apartate aminotransferase (cytoplasmic), Citrate kinase B-type, Fructose-bisphosphate aldolase C, Histone H2A type 1-J, Tubulin alpha-1A chain, Tubulin beta-2C chain and so on. The possibility that can detect the disease more-specific protein will increase by using immunoprecipitation before the analysis.
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