| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Formation of functional neural circuits in developing brain involves activity-dependent refinement of supernumerary connected synapses during early phase of development (synapse elimination). However it is not still completely understand what actual activity of presynaptic neurons in vivo relates to the synapse elimination. Thus, this study focused on the developing “inferior olivary neuron (ION)”, which is a presynaptic neuron in climbing fiber (CF) to Purkinje cell (PC) synapse. To reveal activities of developing IONs in vivo, I established whole-cell patch-clamp recordings from developing IONs in anesthetized mice. And then, I found that around 10 Hz rhythmic firings based on subthreshold membrane oscillations, which have been well known in mature IONs, were observed from 2-3 postnatal weeks. This period involves in the CF-synapse elimination phase, suggesting that the rhythmic activity of ION may be implicated as the potential substrate for CF-synapse elimination.
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