| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Bovine ciliary muscle has unique contractile properties characterized by rapid response to stimuli and sustained contraction. In this study, to address the molecular mechanisms of contraction, I performed phosphorylation analysis by using highly sensitive quantification methods which we have developed. In bovine ciliary muscle, no significant changes were observed in myosin light chain phosphorylation in carbachol-induced or ionomycin-induced contractions regardless of contraction and relaxation. Furthermore, inhibition of myosin light chain kinase could not attenuate contraction, suggesting that ciliary muscle has a unique regulatory mechanisms.
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