Development of highly sensitive methods for phosphorylation analysis
| Project/Area Number |
24700407
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
TAKEYA Kosuke 旭川医科大学, 医学部, 助教 (20586862)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 平滑筋 / 毛様体筋 / リン酸化 / ミオシン / リン酸化定量法 / キナーゼ / ホスファターゼ |
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| Outline of Final Research Achievements |
Bovine ciliary muscle has unique contractile properties characterized by rapid response to stimuli and sustained contraction. In this study, to address the molecular mechanisms of contraction, I performed phosphorylation analysis by using highly sensitive quantification methods which we have developed. In bovine ciliary muscle, no significant changes were observed in myosin light chain phosphorylation in carbachol-induced or ionomycin-induced contractions regardless of contraction and relaxation. Furthermore, inhibition of myosin light chain kinase could not attenuate contraction, suggesting that ciliary muscle has a unique regulatory mechanisms.
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Report
(4 results)
Research Products
(6 results)
