| Project/Area Number |
24700429
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | Osaka University |
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Principal Investigator |
ONO Chikako 大阪大学, 微生物病研究所, 特任研究員(常勤) (30626437)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | C型肝炎ウイルス / 感染症 / マウスモデル / 癌 |
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| Outline of Final Research Achievements |
We established mouse immortalized hepatocyte cell lines that stably expresses four HCV receptor candidates (hCD81, hSR-B1, hCLDN-1, hOCLN) and liver-specific micro RNA, miR-122. Although this cell line permits HCV entry and slight replication of HCV-RNA and virus protein expression were observed upon infection with HCV, efficient replication of HCV-RNA and production of infectious particles were not detected. Then, we could not obtain mouse-adapted HCV that can propagate efficiently in mouse cell lines.
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