Establishment of mouse infection model of HCV

• Project/Area Number: 24700429
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Laboratory animal science
• Research Institution: Osaka University
• Principal Investigator: ONO Chikako 大阪大学, 微生物病研究所, 特任研究員(常勤) (30626437)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: C型肝炎ウイルス / 感染症 / マウスモデル / 癌

Outline of Final Research Achievements

We established mouse immortalized hepatocyte cell lines that stably expresses four HCV receptor candidates (hCD81, hSR-B1, hCLDN-1, hOCLN) and liver-specific micro RNA, miR-122. Although this cell line permits HCV entry and slight replication of HCV-RNA and virus protein expression were observed upon infection with HCV, efficient replication of HCV-RNA and production of infectious particles were not detected. Then, we could not obtain mouse-adapted HCV that can propagate efficiently in mouse cell lines.

Research Products

• [Journal Article] Amphipathic α-Helices in Apolipoproteins Are Crucial to the Formation of Infectious Hepatitis C Virus Particles (2014)
  • Author(s): Fukuhara T, Wada M, Nakamura S, Ono C, Shiokawa M, Yamamoto S, Motomura T, Okamoto T, Okuzaki D, Yamamoto M, Saito I, Wakita T, Koike K, Matsuura Y
  • Journal Title: PLoS Pathog Volume: 10(12) Issue: 12 Pages: e1004534-e1004534
  • DOI: 10.1371/journal.ppat.1004534
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Innate immune response induced by baculovirus attenuates transgene expression in mammalian cells. (2014)
  • Author(s): Ono C., Ninomiya A., Yamamoto S., Abe T., Wen X., Fukuhara T., Sasai M., Yamamoto M., Saitoh T., Satoh T., Kawai T., Ishii J. K., Akira S., Okamoto T., and Matsuura Y.
  • Journal Title: Journal of Virology Volume: 88 Issue: 4 Pages: 2157-2167
  • DOI: 10.1128/jvi.03055-13
  • Peer Reviewed
• [Journal Article] Novel permissive cell lines for complete propagation of hepatitis C virus. (2014)
  • Author(s): Shiokawa M, Fukuhara T, Ono C, Yamamoto S, Okamoto T, Watanabe N, Wakita T, Matsuura Y.
  • Journal Title: J Virol. Volume: 88(10) Issue: 10 Pages: 5578-94
  • DOI: 10.1128/jvi.03839-13
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Characterization of HCV propagation in miR-122 knockout cells (2014)
  • Author(s): Ono C, Fukuhara T, Yamamoto S, Wada M, Okamoto T, Okuzaki D, Matsuura Y
  • Organizer: 第13回あわじしま感染症・免疫フォーラム
  • Place of Presentation: 奈良県新公会堂（奈良）
  • Year and Date: 2014-09-23 – 2014-09-27
• [Presentation] Characterization of HCV Propagation in miR-122 Knockout Cells (2014)
  • Author(s): Ono C, Fukuhara T, Shiokawa M, Yamamoto S, Wada M, Okamoto T, Okuzaki D, Matsuura Y
  • Organizer: 33rd American Society for Virology Annual Meeting
  • Place of Presentation: コロラド（アメリカ合衆国）
  • Year and Date: 2014-06-21 – 2014-06-25
• [Presentation] miR-122ノックアウトHuh7細胞におけるHCV増殖 (2013)
  • Author(s): 小野慎子、福原崇介、塩川 舞、山本聡美、和田真実、岡本 徹、奥崎大介、松浦善治
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸国際会議場（神戸）
• [Presentation] Propagation of HCV in the miR-122-knockout Huh7 cells. (2013)
  • Author(s): Ono C, Fukuhara T, Shiokawa M, Yamamoto S, Wada M, Okamoto T, Okuzaki D, Matsuura Y
  • Organizer: International Symposium on Hepatitis C Virus and Related Viruses
  • Place of Presentation: メルボルン（オーストラリア）
• [Presentation] Innate immuneresponse induced by baculovirus suppresses transgene expression. (2013)
  • Author(s): Ono C, Yamamoto S, Ninomiya A, Fukuhara T, Okamoto T, Abe T, Matsuura Y
  • Organizer: American Society for Virology 32nd Annual Meeting
  • Place of Presentation: ペンシルバニア（アメリカ合衆国）
• [Presentation] C型肝炎ウイルスに感受性を示すマウス肝臓細胞株の樹立
  • Author(s): 小野慎子、福原崇介、塩川 舞、岡本 徹、松浦善治
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: マリンメッセ福岡（ 福岡）
• [Presentation] C型肝炎ウイルスに感受性を示すマウス肝臓細胞株の樹立
  • Author(s): 小野慎子、福原崇介、塩川 舞、岡本 徹、松浦善治
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: グランキューブ大阪（ 大阪）
• [Presentation] Establishment of mouse liver cell lines susceptible to hepatitis C virus infection
  • Author(s): Chikako Ono, Takasuke Fukuhara, Mai Shiokawa, Toru Okamoto, Yoshiharu Matsuura
  • Organizer: The 11th Awaji International Forum on Infection and Immunity
  • Place of Presentation: 淡路夢舞台国際会議場（ 兵庫）