| Project/Area Number |
24700442
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | Tokyo Metropolitan Institute of Medical Science |
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Principal Investigator |
SHIMANUKI Midori 公益財団法人東京都医学総合研究所, 基盤技術研究センター, 基盤技術研究職員 (20593643)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | ミトコンドリア / ミトコンドリアDNA / mtDNA / コピー数 / 遺伝学 / 代謝回転 |
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| Research Abstract |
In this study, we aimed to understand the mechanism that regulates the turnover of mitochondrial DNA (mtDNA), an extra-nuclear genome. First, we demonstrated that the quantity of mtDNA differs among mouse tissues that differ in metabolic properties using real-time PCR. To investigate the factors regulating mtDNA copy number, we temporarily fasted mice to alter the physiological environment. Following fasting, the quantity of mtDNA decreased in some tissues. However, expression of several genes that positively regulate mtDNA copy number increased in most tissues, and the intracellular degradation machinery was activated in the fasted state. These observations indicate that autophagy may be associated with turnover of mtDNA.
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