| Project/Area Number |
24700443
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | Okayama University (2013-2014)
独立行政法人国立精神・神経医療研究センター (2012) |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | マーモセット / 発生工学 |
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| Outline of Final Research Achievements |
In this study, the gene transfer to non-growing oocytes in vitro and the ovarian tissue cryopreservation were carried out in ovaries of common Mmarmoset (Callithrix jacchus) that is a non-human primate. The non-growing oocytes survived for 3 weeks at most. During the time, the non-growing oocytes expressed the GFP fluorescence were found following gene transfer using a lentivirus vector. In ovarian tissue cryopreservation, the tissue after warming maintained the morphology before cryopreservation. There were no significant differences between fresh and vitrification in cryodamage parameters. The TEM analysis revealed the morphlogy equivalent to fresh ovaries in the vitrified/warmed ovaries. This study showed novel possibility of developmental biotechnology in a non-human primate.
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