Mechanism of protrusion during cell migration based on actin dynamics
| Project/Area Number |
24700448
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Biomedical engineering/Biological material science
|
|---|
| Research Institution | Chiba University |
|---|
Principal Investigator |
SUGAWARA Michiko 千葉大学, 工学(系)研究科(研究院), 特任准教授 (30323041)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
|---|
| Keywords | 細胞運動 / アクチンダイナミクス / バイオメカニクス / 細胞 |
|---|
| Research Abstract |
An attempt was made to clarify the mechanism of the formation of protrusion, which is the origin of the cell migration, by combining the numerical analysis of actin dynamics with quantitative measurement of the protrusion formation during migration. As a result of the numerical analysis, various intracellular actin structures could be achieved by the variations on concentration of actin protein and rate constant of nucleation. The experiment on the initiation of cell migration using photoactivatable substrate, in which cells were confined into geometrical polarity and thereby cell migration was induced, revealed that the asymmetric geometry of the cell was a key factor for inducing the cell migration, and that nuclear-centrosomal axis also affect it. In addition, it was suggested that intracellular spatio-temporal dynamics of the formation of focal adhesion together with actin stress fibers play an important role in directionality of cell migration.
|
Report
(3 results)
Research Products
(15 results)
