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Biomarker discovery with wide targeted lipidomic profiling for early detection of colorectal cancer

Research Project

Project/Area Number 24700455
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biomedical engineering/Biological material science
Research InstitutionOsaka University (2013-2014)
Kobe University (2012)

Principal Investigator

IZUMI Yoshihiro  大阪大学, 工学(系)研究科(研究院), 助教 (70622166)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsメタボロミクス / リピドミクス / 大腸がん / バイオマーカー探索 / 慢性炎症 / メタボローム解析 / 脂質 / バイオマーカー
Outline of Final Research Achievements

To improve the quality of life of colorectal cancer patients, it is important to establish new screening methods for early diagnosis of colorectal cancer. Lipids are fundamental constituents that structurally and chemically regulate cell membranes, store energy, and can become precursors to bioactive metabolites. We developed the comprehensive lipids profiling methodology and performed serum lipidomic analysis using liquid chromatography/tandem mass spectrometry (LC/MSMS). Wide targeted lpidomic profiling was not only provide insights into the specific roles of molecular species for enteritis and colorectal cancer, but also assist in identifying potential biomarkers for colorectal cancer.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (47 results)

All 2015 2014 2013 2012 Other

All Journal Article (20 results) (of which Peer Reviewed: 20 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (24 results) (of which Invited: 3 results) Book (2 results) Remarks (1 results)

  • [Journal Article] MRM-DIFF: Data Processing Strategy for Differential Analysis in Large Scale MRM-based Lipidomics Studies.2015

    • Author(s)
      Tsugawa H, Ohta E, Izumi Y, Ogiwara A, Yukihira D, Bamba T, Fukusaki E, Arita M.
    • Journal Title

      Frontiers in Genetics.

      Volume: 5 Pages: 471-471

    • DOI

      10.3389/fgene.2014.00471

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] High-throughput simultaneous analysis of pesticides by supercritical fluid chromatography coupled to high-resolution mass spectrometry.2015

    • Author(s)
      Ishibashi M, Izumi Y, Sakai M, Ando T, Fukusaki E, Bamba T.
    • Journal Title

      J. Agric. food Chem.

      Volume: in press

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lipidomic analysis of plasma lipoprotein fractions in myocardial infarction-prone rabbits.2015

    • Author(s)
      Takeda H, Koike T, Izumi Y, Takayuki Yamada T, Yoshida M, Shiomi M, Fukusaki E, Bamba T.
    • Journal Title

      J. Biosci. Bioeng.

      Volume: in press

    • NAID

      110010010234

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Practical evaluation of liquid chromatography/tandem mass spectrometry and enzyme immunoassay method for the accurate quantitative analysis of prostaglandins.2014

    • Author(s)
      Izumi Y, Aritake K, Urade Y, Fukusaki E.
    • Journal Title

      J. Biosci. Bioeng.

      Volume: 118(1) Issue: 1 Pages: 116-118

    • DOI

      10.1016/j.jbiosc.2013.12.022

    • NAID

      110009838781

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Supercritical fluid extraction as a preparation method for mass spectrometry of dried blood spots.2014

    • Author(s)
      Matsubara A, Izumi Y, Nishiumi S, Suzuki M, Azuma T, Fukusaki E, Bamba T, Yoshida M.
    • Journal Title

      J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.

      Volume: 969 Pages: 199-204

    • DOI

      10.1016/j.jchromb.2014.08.013

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Butyrate Attenuates Inflammation and Lipolysis Generated by the Interaction of Adipocytes and Macrophages2013

    • Author(s)
      Ohira H, Fujioka Y, Katagiri C, Mamoto R, Aoyama M, Izumi Y, Nishiumi S, Yoshida M, Usami M, Ikeda M.
    • Journal Title

      Journal of Atherosclerosis and Thrombosis

      Volume: 20 Issue: 5 Pages: 425-442

    • DOI

      10.5551/jat.15065

    • NAID

      130004444681

    • ISSN
      1340-3478, 1880-3873
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Dynamic metabolic profiling of cyanobacterial glycogen biosynthesis under conditions of nitrate depletion.2013

    • Author(s)
      Hasunuma T, Kikuyama F, Matsuda M, Aikawa S, Izumi Y, Kondo A.
    • Journal Title

      J. Exp. Bot.

      Volume: 64(10) Issue: 10 Pages: 2943-2954

    • DOI

      10.1093/jxb/ert134

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Aqueous size-exclusion chromatographic method for the quantification of cyanobacterial native glycogen.2013

    • Author(s)
      Izumi Y, Aikawa S, Matsuda F, Hasunuma T , Kondo A.
    • Journal Title

      J. Chromatogr. B

      Volume: 930 Pages: 90-97

    • DOI

      10.1016/j.jchromb.2013.04.037

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Direct conversion of Spirulina to ethanol without pretreatment or enzymatic hydrolysis processes.2013

    • Author(s)
      Aikawa S, Joseph A, Yamada R, Izumi Y, Yamagishi T, Matsuda F, Kawai H, Chang JS, Hasunuma T, Kondo A.
    • Journal Title

      Energy Environ. Sci.

      Volume: 6 Issue: 6 Pages: 1844-1849

    • DOI

      10.1039/c3ee40305j

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] A novel serum metabolomics-based diagnostic approach to pancreatic cancer.2013

    • Author(s)
      Kobayashi T, Nishiumi S, Ikeda A, Yoshie T, Sakai1 A, Matsubara A, Izumi Y, Tsumura H, Tsuda M, Nishisaki H, Hayashi N, Kawano S, Fujiwara Y, Minami H, Takenawa T, Azuma T, Yoshida M.
    • Journal Title

      Cancer Epidem. Biomar.

      Volume: 22(4) Issue: 4 Pages: 571-579

    • DOI

      10.1158/1055-9965.epi-12-1033

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Application of ion mobility-mass spectrometry to microRNA analysis.2013

    • Author(s)
      Takebayashi K, Hirose K, Izumi Y, Bamba T, Fukusaki E.
    • Journal Title

      J. Biosci. Bioeng.

      Volume: 115(3) Issue: 3 Pages: 332-338

    • DOI

      10.1016/j.jbiosc.2012.10.006

    • NAID

      110009596110

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Microbiota-derived lactate accelerates colon epithelial cell turnover in starvation-refed mice2013

    • Author(s)
      Okada T
    • Journal Title

      Nat. Commun

      Volume: 4巻 Issue: 1 Pages: 1654-1654

    • DOI

      10.1038/ncomms2668

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effect of gallic acid on peptides released by trypsin digestion of bovine a-casein. J.2013

    • Author(s)
      Lai P, Okazawa A, Izumi Y, Bamba T, Fukusaki E, Yoshikawa M, Kobayashi A
    • Journal Title

      J. Biosci. Bioeng.

      Volume: 115(3) Issue: 3 Pages: 259-267

    • DOI

      10.1016/j.jbiosc.2012.10.003

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] A novel serum metabolomics-based diagnostic approach for colorectal cancer.2012

    • Author(s)
      Nishiumi S, Kobayashi T, Ikeda A, Yoshie T, Kibi M, Izumi Y, Okuno T, Hayashi N, Kawano S, Takenawa T, Azuma T, Yoshida M.
    • Journal Title

      PLoS ONE

      Volume: 7(7) Issue: 7 Pages: e40459-e40459

    • DOI

      10.1371/journal.pone.0040459

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Metabolomic analysis to discover candidate therapeutic agents against acute pancreatitis.2012

    • Author(s)
      Nishiumi S, Shiomi Y, Kobayashi T, Izumi Y, Kutsumi H, Hayakumo T, Azuma T, Yoshida M.
    • Journal Title

      Arch. Biochem. Biophys.

      Volume: 522(2) Issue: 2 Pages: 107-120

    • DOI

      10.1016/j.abb.2012.03.025

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Regulation of the metabolite profile by an APC gene mutation in colorectal cancer.2012

    • Author(s)
      Yoshie T, Nishiumi S, Izumi Y, Sakai A, Inoue J, Azuma T, Yoshida M.
    • Journal Title

      Cancer Sci.

      Volume: 103(6) Issue: 6 Pages: 1010-21

    • DOI

      10.1111/j.1349-7006.2012.02262.x

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Diagnosis of gastroenterological diseases by metabolome analysis using gas chromatography-mass spectrometry2012

    • Author(s)
      Yoshida M, Hatano N, Nishiumi S, Irino Y, Izumi Y, Takenawa T, Azuma T
    • Journal Title

      J. Gastroenterol

      Volume: 47(1) Issue: 1 Pages: 9-20

    • DOI

      10.1007/s00535-011-0493-8

    • NAID

      10030295488

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Application of electrospray ionization ion trap/time-of-flight mass spectrometry for chemically-synthesized small RNAs.2012

    • Author(s)
      Izumi Y, Takimura S, Yamaguchi S, Iida J, Bamba T, Fukusaki E.
    • Journal Title

      J. Biosci. Bioeng.

      Volume: 113(3) Issue: 3 Pages: 412-419

    • DOI

      10.1016/j.jbiosc.2011.11.007

    • NAID

      110009425337

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Widely targeted metabolic profiling analysis of yeast central metabolites2012

    • Author(s)
      Kato H, Izumi Y., Hasunuma T, Matsuda F, Kondo A
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: Vol.113 Issue: 5 Pages: 665-673

    • DOI

      10.1016/j.jbiosc.2011.12.013

    • NAID

      110009456911

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Gallic acid oxidizes Met-residues in peptides released from bovine β-lactoglobulin by in vitro digestion.2012

    • Author(s)
      Lai P, Okazawa A, Izumi Y, Bamba T, Fukusaki E, Yoshikawa M, Kobayashi A
    • Journal Title

      J. Biosci. Bioeng.

      Volume: 114(3) Issue: 3 Pages: 297-305

    • DOI

      10.1016/j.jbiosc.2012.04.019

    • NAID

      110009517381

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] SFC/MSを用いた残留農薬分析2015

    • Author(s)
      和泉自泰
    • Organizer
      第12回SFC研究会
    • Place of Presentation
      東京
    • Year and Date
      2015-02-06
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 超臨界流体クロマトグラフィー質量分析の代謝プロファイリングへの応用2014

    • Author(s)
      和泉自泰
    • Organizer
      高分子分析研究懇談会第374回例会
    • Place of Presentation
      京都
    • Year and Date
      2014-09-11
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] オンライン超臨界流体抽出 - 超臨界流体クロマトグラフィー / 質量分析を用いた脂溶性ビタミンの一斉分析系の構築2014

    • Author(s)
      上野真菜子,和泉自泰,福崎英一郎,馬場健史
    • Organizer
      第66回日本生物工学会大会
    • Place of Presentation
      札幌
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] 高脂肪食マウスを対象とした脂質メタボローム解析2014

    • Author(s)
      山田 貴之, 和泉 自泰, 西海 信, 吉田 優, 福崎 英一郎, 馬場 健史
    • Organizer
      第66回日本生物工学会大会
    • Place of Presentation
      札幌
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] 安定同位体標識化合物を用いた代謝ターンオーバー解析2014

    • Author(s)
      和泉自泰
    • Organizer
      第147回質量分析関西談話会
    • Place of Presentation
      大阪
    • Year and Date
      2014-08-09
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] High-throughput and high-resolution lipidomics platform using supercritical fluid chromatography coupled to Orbitrap mass spectrometry.2014

    • Author(s)
      Takayuki Yamada, Yoshihiro Izumi, Shigeru Sakamoto, Yasuto Yokoi, Eiichiro Fukusaki, Takeshi Bamba
    • Organizer
      Metabolomics 2014
    • Place of Presentation
      Tsuruoka, Japan
    • Year and Date
      2014-06-23 – 2014-06-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] Lipidome analysis of plasma, livers, and adipose tissues in high-fat-diet-fed mice using supercritical fluid chromatography coupled to high-resolution mass spectrometry2014

    • Author(s)
      Takayuki Yamada, Yoshihiro Izumi, Shin Nishiumi, Masaru Yoshida, Eiichiro Fukusaki, Takeshi Bamba
    • Organizer
      62nd ASMS Conference
    • Place of Presentation
      Baltimore, MD, USA
    • Year and Date
      2014-06-15 – 2014-06-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] オンライン超臨界流体抽出-超臨界流体クロマトグラフィー質量分析を用いた脂溶性ビタミンの一斉分析系の構築2014

    • Author(s)
      上野真菜子,和泉自泰,福崎英一郎,馬場健史
    • Organizer
      第62回質量分析総合討論会
    • Place of Presentation
      大阪
    • Year and Date
      2014-05-14 – 2014-05-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] ブレオマイシン誘発肺線維症モデルラットを対象とした脂質メタボローム解析2014

    • Author(s)
      山田貴之,和泉自泰,坂東清子,福崎英一郎,馬場健史
    • Organizer
      第62回質量分析総合討論会
    • Place of Presentation
      大阪
    • Year and Date
      2014-05-14 – 2014-05-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] 心筋梗塞自然発症モデルウサギのリポプロテイン脂質プロファイリング2014

    • Author(s)
      竹田浩章、小池智也、山田貴之、和泉自泰、塩見雅志、吉田優、福崎英一郎、馬場健史
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学 生田キャンパス
    • Related Report
      2013 Research-status Report
  • [Presentation] ブレオマイシン誘発肺線維症モデルラットの肺サーファクタントを対象とした脂質メタボローム解析2013

    • Author(s)
      山田貴之、和泉自泰、坂東清子、福崎英一郎、馬場 健史
    • Organizer
      第8回 メタボロームシンポジウム
    • Place of Presentation
      九州大学医学部 百年講堂
    • Related Report
      2013 Research-status Report
  • [Presentation] 高速高分離超臨界クロマトグラフィー質量分析システムによるリピドーム解析2013

    • Author(s)
      和泉自泰、山田貴之、福崎英一郎、馬場 健史
    • Organizer
      第8回 メタボロームシンポジウム
    • Place of Presentation
      九州大学医学部 百年講堂
    • Related Report
      2013 Research-status Report
  • [Presentation] 超臨界流体クロマトグラフィー/タンデム質量分析によるハイスループット脂質メタボロミクスシステムの開発2013

    • Author(s)
      山田貴之、和泉自泰、福崎英一郎、馬場健史
    • Organizer
      第61回質量分析総合討論会
    • Place of Presentation
      つくば国際会議場
    • Related Report
      2013 Research-status Report
  • [Presentation] メタボロミクスを用いた早期大腸癌診断法2013

    • Author(s)
      吉田 優, 西海 信, 和泉 自泰, 松原 惇起, 東 健
    • Organizer
      日本薬学会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] クローン病モデルIL-10ノックアウトマウスのメタボローム解析2012

    • Author(s)
      和泉 自泰, 西海 信, 松原 惇起, 東 健,吉田 優
    • Organizer
      第7回メタボロームシンポジウム
    • Place of Presentation
      鶴岡
    • Related Report
      2012 Research-status Report
  • [Presentation] Serum metabolome analysis for early detection of colorectal cancer2012

    • Author(s)
      Masaru Yoshida, Shin Nishiumi, Yoshihiro Izumi, Atsuki Matsubara, Takeshi Azuma
    • Organizer
      国際分析質量会議
    • Place of Presentation
      京都
    • Related Report
      2012 Research-status Report
  • [Presentation] Serum metabolomic profiling for early detection of colorectal cancer2012

    • Author(s)
      Masaru Yoshida, Shin Nishiumi, Yoshihiro Izumi, Atsuki Matsubara, Takeshi Azuma
    • Organizer
      Metabomeeting
    • Place of Presentation
      Manchester, UK
    • Related Report
      2012 Research-status Report
  • [Presentation] GC/MS-based profiling of amino acids and TCA cycle-related molecules in ulcerative colitis2012

    • Author(s)
      Masaru Yoshida, Makoto Ooi, Shin Nishiumi, Yoshihiro Izumi, Takeshi Azuma
    • Organizer
      Metabolomics
    • Place of Presentation
      Washington, D.C.
    • Related Report
      2012 Research-status Report
  • [Presentation] A novel serum metabolomics-based diagnostic approach for colorectal cancer2012

    • Author(s)
      Masaru Yoshida, Shin Nishiumi, Yoshihiro Izumi, Takeshi Azuma
    • Organizer
      日本癌学会
    • Place of Presentation
      札幌
    • Related Report
      2012 Research-status Report
  • [Presentation] メタボロミクスによる疾患バイオマーカーの探索2012

    • Author(s)
      吉田 優, 西海 信, 和泉 自泰, 東 健
    • Organizer
      日本認知症学会
    • Place of Presentation
      筑波
    • Related Report
      2012 Research-status Report
  • [Presentation] 血清メタボローム解析による新規大腸がんスクリーニングシステムの開発2012

    • Author(s)
      吉田 優, 西海 信, 和泉 自泰, 松原 惇起, 東 健
    • Organizer
      日本医用マススペクトル学会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Research-status Report
  • [Presentation] メタボロミクスを用いた炎症性疾患に対する新規治療薬の探索への試み2012

    • Author(s)
      西海 信, 和泉 自泰, 松原 惇起, 東 健,吉田 優
    • Organizer
      日本医用マススペクトル学会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Research-status Report
  • [Presentation] 血清メタボローム解析による大腸がん診断システムの開発2012

    • Author(s)
      吉田 優, 西海 信, 和泉 自泰, 松原 惇起, 東 健
    • Organizer
      第7回メタボロームシンポジウム
    • Place of Presentation
      鶴岡
    • Related Report
      2012 Research-status Report
  • [Presentation] ブレオマイシン誘発肺障害の発症機序とバイオマーカーの探索-脂質成分とプロスタグランジンの変動2012

    • Author(s)
      坂東清子、紺屋豊、梅村康士、和泉自泰、国松武史、木村重紀、船橋斉、馬場健史、福崎英一郎
    • Organizer
      毒性学会
    • Place of Presentation
      仙台
    • Related Report
      2012 Research-status Report
  • [Book] Mass Spectrometry-Based Metabolomics: A Practical Guide, Chapter 3: Sample Preparation.2014

    • Author(s)
      Izumi Y, Lavina WA, Putri SP.
    • Total Pages
      66
    • Publisher
      CRC Press, Boca Raton, Florida, USA
    • Related Report
      2014 Annual Research Report
  • [Book] 超臨界流体抽出分離技術を用いたリピドーム解析手法の開発2014

    • Author(s)
      和泉自泰,福崎英一郎,馬場 健史
    • Total Pages
      7
    • Publisher
      オレオサイエンス
    • Related Report
      2014 Annual Research Report
  • [Remarks] 神戸大学大学院医学研究科 疾患メタボロミクス分野

    • URL

      http://www.med.kobe-u.ac.jp/metabo/kenkyu.html

    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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