Development of enzyme-loaded nano-sized polyion complex vesicles (PICsomes) for enzyme prodrug therapy

• Project/Area Number: 24700476
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biological material science
• Research Institution: The University of Tokyo
• Principal Investigator: ANRAKU Yasutaka 東京大学, 工学(系)研究科(研究院), 助教 (60581585)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: Enzyme/Prodrug Therapy / ナノリアクター / PICsome / 制がん剤 / In vivoナノリアクター / 酵素プロドラッグ療法 / 抗腫瘍効果 / β-ガラクトシダーゼ / シトシンデアミナーゼ / 静電相互作用型ベシクル / 腫瘍

Research Abstract

Utilization of enzymes in the body is still a big challenge in the biomedical field. One of promising applications of enzymes for therapeutic application is enzyme prodrug therapy, and development of nano-vehicles for enzymes is one effective clue to establish the golden standard of EPT. We succeed in the preparation of b-galactosidase loaded PICsome with the diameter of 100 nm, maintaining enzyme activity. Exploiting b-gal-loaded PICsomes as an enzyme vehicles, a model prodrug, HMDER-bGAL, was successfully converted into highly fluorescent product, HMDER, due to permeation of HMDER-bGAL through the vesicle wall. In in vivo experiments using tumor-bearing mice, b-gal-loaded PICsomes were accumulated in the tumor tissue, to produce HMDER. Resulted HMDER was released form PICsomes and distributed into the entire tumor tissues and internalized by cells, and then visualization of tumor tissues was performed.

Research Products

• [Journal Article] Morphology control in water of polyion complex nanoarchitectures of double-hydrophilic charged block copolymers through composition tuning and thermal treatment (2014)
  • Author(s): A. Wibowo, K. Osada, H. Matsuda, Y. Anraku, H. Hirose, A. Kishimura, K. Kataoka
  • Journal Title: Macromolecules Volume: 47 (9) Issue: 9 Pages: 3086-3092
  • DOI: 10.1021/ma500314d
  • Peer Reviewed
• [Journal Article] Structural factors directing to nanosized polyion complex vesicles (Nano-PICsomes) from a pair of block aniomer/homo catiomer : studies on aniomer segment length and catiomer side chain structure (2014)
  • Author(s): Sayan Chuanoi, Wen-Fei Dong, Akihiro Kishimura, Yasutaka Anraku, and Kazunori Kataoka
  • Journal Title: Polymer Journal Volume: 46 Issue: 2 Pages: 130-135
  • DOI: 10.1038/pj.2013.82
  • Peer Reviewed
• [Journal Article] Hydrothermally synthesized PEGylated calcium phosphate nanoparticles incorporating Gd-DTPA for contrast enhanced MRI diagnosis of solid tumors (2014)
  • Author(s): P. Mi, N. Nishiyama, et al.
  • Journal Title: J. Control. Release Volume: 136 Issue: 1 Pages: 157-163
  • DOI: 10.1021/ja406992w
  • Peer Reviewed
• [Journal Article] SPIO-PlCsome : Development of a highly sensitive and stealth-capable MRI nano-agent for tumor detection using SPIO-loaded unilamellar polyion complex vesicles (PICsomes) (2013)
  • Author(s): D. Kokuryo, N. Nishiyama, et al.
  • Journal Title: J. Control. Release Volume: 169 Issue: 3 Pages: 220-227
  • DOI: 10.1016/j.jconrel.2013.03.016
  • Peer Reviewed
• [Journal Article] Living unimodal growth of polyion complex vesicles via 2D supramolecular polymerization (2013)
  • Author(s): Y. Anraku, A. Kishimura, Y. Yamasaki, K. Kataoka
  • Journal Title: J. Am. Chem. Soc. Volume: 135 (4) Issue: 4 Pages: 1423-1429
  • DOI: 10.1021/ja3096587
  • Peer Reviewed
• [Journal Article] Smart multilayered assembly for biocompatible siRNA delivery featuring dissolvable silica, endosome-disrupting polycation, and detachable PEG (2012)
  • Author(s): T. Suma, K. Miyata
  • Journal Title: ACS Nano Volume: 6 Issue: 8 Pages: 6693-6705
  • DOI: 10.1021/nn301164a
  • Peer Reviewed
• [Journal Article] Bioactivepolymeric metallosomesself-assembled through blockcopolymer-metal complexation. (2012)
  • Author(s): K. Osada, H. Cabral, Y. Mochida, S. -ELee, K. Nagata, T. Matsuura, M.Yamamoto, Y. Anraku, A. Kishimura, N.Nishiyama, K. Kataoka
  • Journal Title: J. Am.Chem. Soc. Volume: 134(32) Issue: 32 Pages: 13172-13175
  • DOI: 10.1021/ja304615y
  • Peer Reviewed
• [Presentation] Development of enzyme-loaded nano-sized polyion complex vesicles (PICsomes) for enzyme prodrug therapy FIRST合同国際シンポジウム難治がんを克服する―次世代の診断、治療が描く未来 (2014)
  • Author(s): Y. Anraku, M. Kamiya, S. Tanaka, T. Nomoto, K. Toh, Y. Matsumoto, S. Fukushima, A. Kishimura, M. R. Kano, Y. Urano, N. Nishiyama, K. Kataoka
  • Organizer: Overcoming Intractable Cancer- a new path to the future
  • Place of Presentation: 伊藤国際学術研究センター文京区、東京都
  • Year and Date: 2014-02-21
• [Presentation] Development of enzyme-loaded nano- sized polyion complex vesicles (PICsomes) for enzyme/prodrug therapy (2013)
  • Author(s): Y. Anraku, A. Kishimura, M. Kamiya, S. Tanaka, T. Nomoto, K. Toh, Y. Matsumoto, M. R. Kano, Y. Urano, N. Nishiyama, K. Kataoka
  • Organizer: 第35回日本バイオマテリアル学会大会
  • Place of Presentation: タワーホール船堀、江戸川、東京
  • Year and Date: 2013-11-25
• [Presentation] ポリイオンコンプレックス(PIC)型ベシクルのユニークな動的挙動とその機能 (2013)
  • Author(s): 安楽泰孝、岸村顕広、片岡一則
  • Organizer: 第62回高分子学会討論会
  • Place of Presentation: 金沢大学角間キャンパス、金沢、石川
  • Year and Date: 2013-09-11
• [Presentation] PIC型ベシクルの特徴を活かした酵素プロドラッグ療法用キャリアの構築 (2013)
  • Author(s): 安楽泰孝、岸村顕広、神谷真子、田中さやか、野本貴大、藤加珠子、松本有、狩野光伸、浦野泰照、西山伸宏、片岡一則
  • Organizer: 遺伝子・デリバリー研究会第13回夏期セミナー
  • Place of Presentation: Hawaii Prince Hotel Waikiki, Honolulu, Hawaii, USA
  • Year and Date: 2013-07-24
• [Presentation] Development of enzyme-loaded PICsomes for enzyme (2013)
  • Author(s): Y. Anraku, M. Kamiya, S. Tanaka, T. Nomoto, K. Toh, Y. Matsumoto, A. Kishimura, M. R. Kano, Y. Urano, N. Nishiyama, K. Kataoka
  • Organizer: prodrug therapy 40th Annual Meeting & Exposition of the Controlled Release Society
  • Place of Presentation: Hawaii Convention Center, Honolulu, Hawaii, USA
  • Year and Date: 2013-07-21
• [Presentation] 汎用性の高い酵素プロドラッグ療法用キャリアを指向した血中滞留型中空ナノ粒子(PICsome)の構築 (2013)
  • Author(s): 安楽泰孝、岸村顕広、神谷真子、田中さやか、野本貴大、藤加珠子、松本有、狩野光伸、浦野泰照、西山伸宏、片岡一則
  • Organizer: 第29回日本DDS学会学術集会
  • Place of Presentation: 京都テルサ、京都市、京都府
  • Year and Date: 2013-07-04
• [Presentation] Development of nano-sized polyion complex vesicles (Nano-PICsomes) as a versatile delivery carrier for biomedical application FRONTIERS2013 - Joint EPFL (2013)
  • Author(s): Y. Anraku, A. Kishimura, D. Kokuryo, S. Tanaka, M. Kamiya, K. Tou, T. Nomoto, Y. Matsumoto, I. Aoki, M. Kano, Y. Urano, K. Kataoka
  • Organizer: University of Tokyo Symposium on "Frontiers in Nanomedicine and Imaging" Swiss Federal Institute of Technology
  • Place of Presentation: Lausanne, Switzerland
  • Year and Date: 2013-06-21
• [Presentation] PIC型ベシクルの特徴を活かした酵素プロドラッグ療法用キャリアの構築 (2013)
  • Author(s): 安楽泰孝、岸村顕広、神谷真子、田中さやか、野本貴大、藤加珠子、松本有、狩野光伸、浦野泰照、西山伸宏、片岡一則
  • Organizer: 第62回高分子年次大会
  • Place of Presentation: 京都国際会館、京都市、京都府
  • Year and Date: 2013-05-29
• [Presentation] Development of enzyme-loaded PICsomes as an in vivo reactor (2013)
  • Author(s): Y. Anraku
  • Organizer: Nanobio FIRST International Symposium between Sweden and Japan Achieving medical innovation by nanobio Bench to Bedside, Bedside to Bench, Ito Hall, Ito International Research Center
  • Place of Presentation: The University of Tokyo, Tokyo, Japan
  • Year and Date: 2013-03-05
• [Presentation] Development of nano-sized polyion complex vesicles (Nano-PICsomes) for versatile DDS carriers (2012)
  • Author(s): Y. Anraku, A. Kishimura, D. Kokuryo, S. Tanaka, M. Kamiya, K. Toh, T. Nomoto, Y. Matsumoto, I. Aoki, M. Kano, Y. Urano, K. Kataoka
  • Organizer: 2012 MRS Fall Meeting
  • Place of Presentation: Hynes Convention Center, Boston, Massachusetts, USA
  • Year and Date: 2012-11-26
• [Presentation] Devrlopment of nano-sized polyion complex vesicles (Nano-PICsome) for versatile DDS carriers (2012)
  • Author(s): Y. Anraku, A. Kishimura, K. Kataoka
  • Organizer: NanoBio Seattle 2012 (The Fourth International NanoBio Conference)
  • Place of Presentation: Bell Harbor International Convention Center, Seattle, USA
  • Year and Date: 2012-07-23
• [Presentation] In vivoナノリアクターへの展開を指向した酵素封入PICsomeの機能評価 (2012)
  • Author(s): 安楽泰孝、岸村顕広、田中さやか、神谷真子、狩野光伸、浦野泰照、片岡一則
  • Organizer: 第28回日本DDS学会学術集会
  • Place of Presentation: 札幌コンベンションセンター、北海道
  • Year and Date: 2012-07-04
• [Presentation] 汎用性の高いDDSキャリアを指向した中空型ナノデバイスの開発 (2012)
  • Author(s): 安楽泰孝
  • Organizer: ナノバイオ国際共同研究教育拠点第1回若手国内シンポジウムNanobio第5回若手ネットワーキングシンポジウム
  • Place of Presentation: 甲南大学フロンティアサイエンス学部レクチャールーム
  • Year and Date: 2012-06-08
• [Presentation] 架橋率・サイズの異なるポリイオンコンプレックス型中空粒子(Nano-PICsome)の基礎物性および体内動態評価 (2012)
  • Author(s): 安楽泰孝、田中さやか、岸村顕広、狩野光伸、長田健介、片岡一則
  • Organizer: 第61回高分子学会年次大会
  • Place of Presentation: パシフィコ横浜、神奈川県
  • Year and Date: 2012-05-29
• [Presentation] Development of nano‐sized polyion complex vesicles (Nano‐PICsomes) as a versatile delivery carrier for biomedical application
  • Author(s): Y. Anraku, A. Kishimura, D. Kokuryo, S. Tanaka, M. Kamiya, K. Tou, T. Nomoto, Y. Matsumoto, I. Aoki, M. Kano, Y. Urano, K. Kataoka
  • Organizer: FRONTIERS2013 - Joint EPFL/University of Tokyo Symposium on "Frontiers in Nanomedicine and Imaging"
  • Place of Presentation: Lausanne, Switzerland
• [Presentation] Development of mesoporous silica nanoparticle (MSN)-loaded PICsomes for delivery of low molecular-weight drugs
  • Author(s): A. Goto, K. Kataoka, A. Kishimura, Y. Anraku
  • Organizer: 40th Annual Meeting & Exposition of the Controlled Release Society
  • Place of Presentation: Hawaii, USA
• [Presentation] Development of enzyme-loaded PICsomes for enzyme/prodrug therapy
  • Author(s): Y. Anraku, M. Kamiya, S. Tanaka, T. Nomoto, K. Toh, Y. Matsumoto, A. Kishimura, M. R. Kano, Y. Urano, N. Nishiyama, K. Kataoka
  • Organizer: 40th Annual Meeting & Exposition of the Controlled Release Society
  • Place of Presentation: Hawaii, USA
• [Presentation] Novel method to load high amount of drugs and macromolecules into polyion complex vesicles (PICsomes)
  • Author(s): A.Kishimura, Y. Anraku, A. Goto, D. Sueyoshi, L. Xiao, N. Nishiyama, K. Kataoka
  • Organizer: 40th Annual Meeting & Exposition of the Controlled Release Society
  • Place of Presentation: Hawaii, USA
• [Presentation] Photosensitizer encapsulated nano polyion complex vesicles (Nano-PICsome) for enhanced in vivo photodynamic therapy
  • Author(s): L. Xiao, H. Chen, Y. Anraku, X. Liu, H. Wu, H. Cabral, K. Tou, Y. Matsumoto, T. Nomoto, K. Akihiro, N. Nishiyama, K. Kataoka
  • Organizer: The 12th US-Japan Symposium on Drug Delivery Systems
  • Place of Presentation: Hawaii, USA
• [Presentation] Development of enzyme-loaded nano-sized polyion complex vesicles (PICsomes) for enzyme prodrug therapy
  • Author(s): Y. Anraku, M. Kamiya, S. Tanaka, T. Nomoto, K. Toh, Y. Matsumoto, S. Fukushima, A. Kishimura, M. R. Kano, Y. Urano, N. Nishiyama, K. Kataoka
  • Organizer: FIRST合同国際シンポジウム 難治がんを克服する―次世代の診断、治療が描く未来 Overcoming Intractable Cancer- a new path to the future
  • Place of Presentation: 伊藤国際学術研究センター (東京都文京区)
• [Presentation] PIC型ベシクルの特徴を活かした酵素プロドラッグ療法用キャリアの構築
  • Author(s): 安楽泰孝、岸村顕広、神谷真子、田中さやか、野本貴大、藤加珠子、松本有、狩野光伸、浦野泰照、西山伸宏、片岡一則
  • Organizer: 第62回高分子年次大会
  • Place of Presentation: 京都国際会館 (京都府京都市)
• [Presentation] 汎用性の高い酵素プロドラッグ療法用キャリアを指向した血中滞留型中空ナノ粒子（PICsome）の構築
  • Author(s): 安楽泰孝、岸村顕広、神谷真子、田中さやか、野本貴大、藤加珠子、松本有、狩野光伸、浦野泰照、西山伸宏、片岡一則
  • Organizer: 第29回日本DDS学会学術集会
  • Place of Presentation: 京都テルサ (京都府京都市)
• [Presentation] 高分子型ベシクルPICsomeへのユニークな物質封入法の開発
  • Author(s): 岸村顕広、安楽泰孝、後藤晃範、片岡一則
  • Organizer: 第29回日本DDS学会学術集会
  • Place of Presentation: 京都テルサ (京都府京都市)
• [Presentation] PIC 型ベシクルの特徴を活かした酵素プロドラッグ療法用キャリアの構築
  • Author(s): 安楽泰孝、岸村顕広、神谷真子、田中さやか、野本貴大、藤加珠子、松本有、狩野光伸、浦野泰照、西山伸宏、片岡一則
  • Organizer: 遺伝子・デリバリー研究会 第13回夏期セミナー
  • Place of Presentation: Hawaii, USA
• [Presentation] 低分子薬物の吸着を可能とする多孔質シリカナノ粒子内封PICsomeの作製とその生理活性評価
  • Author(s): 後藤晃範、安楽泰孝、岸村顕広、片岡一則
  • Organizer: 第62回高分子学会討論会
  • Place of Presentation: 金沢大学角間キャンパス (石川県金沢市)
• [Presentation] ポリイオンコンプレックス(PIC)型ベシクル(PICsome)のPIC層の機能化と細胞取込み挙動の制御
  • Author(s): 柿山創、安楽泰孝、岸村顕広、片岡一則
  • Organizer: 第62回高分子学会討論会
  • Place of Presentation: 金沢大学角間キャンパス (石川県金沢市)
• [Presentation] ポリイオンコンプレックス型ベシクルPICsomeを用いた新規生体イメージング／薬物送達手法の開発
  • Author(s): 岸村顕広、安楽泰孝、柿山創、M. Omer, 後藤晃範、片岡一則
  • Organizer: 第7回バイオ関連化学シンポジウム
  • Place of Presentation: 名古屋大学東山キャンパス 豊田講堂・野依学術交流館 (愛知県名古屋市)
• [Presentation] Development of enzyme-loaded nano- sized polyion complex vesicles (PICsomes) for enzyme/prodrug therapy
  • Author(s): Y. Anraku, A. Kishimura, M. Kamiya, S. Tanaka, T. Nomoto, K. Toh, Y. Matsumoto, M. R. Kano, Y. Urano, N. Nishiyama, K. Kataoka
  • Organizer: 第35回日本バイオマテリアル学会大会
  • Place of Presentation: タワーホール船堀 (東京都江戸川区)
• [Presentation] ポリイオンコンプレックス型ベシクル (PICsome)のPIC層の 修飾を通じた細胞取込み挙動の制御
  • Author(s): 柿山創、安楽泰孝、岸村顕広、片岡一則
  • Organizer: 第23回日本MRS年次大会
  • Place of Presentation: 横浜市開港記念会館 (神奈川県横浜市)
• [Presentation] Development of Polyion Complex Vesicles and its Application to Nano-medicine
  • Author(s): A. Kishimura, Y. Anraku, S. Chuanoi, K. Kataoka
  • Organizer: IACIS 2012
  • Place of Presentation: 仙台国際センター、宮城県
• [Presentation] 化学修飾したポリイオンコンプレックス膜を持つPICsomeの細胞取込挙動
  • Author(s): 柿山創、安楽泰孝、岸村顕広、片岡一則
  • Organizer: 第61回高分子学会年次大会
  • Place of Presentation: パシフィコ横浜、神奈川県
• [Presentation] アクティブターゲッティングDDSを目指した表面修飾PICsomeの構築と評価
  • Author(s): 川村渉、安楽泰孝、岸村顕広、片岡一則
  • Organizer: 第61回高分子学会年次大会
  • Place of Presentation: パシフィコ横浜、神奈川県
• [Presentation] Y字型ブロック共重合体を用いたポリイオンコンプレックスの作製とその性質
  • Author(s): 小林敦、岸村顕広、S. -E.. Lee、安楽泰孝、比木茂寛、片岡一則
  • Organizer: 第61回高分子学会年次大会
  • Place of Presentation: パシフィコ横浜、神奈川県
• [Presentation] 汎用性の高いDDSキャリアを指向した中空型ナノデバイスの開発
  • Author(s): 安楽泰孝
  • Organizer: ナノバイオ国際共同研究教育拠点 第1回若手国内シンポジウム Nanobio 第5回若手ネットワーキングシンポジウム
  • Place of Presentation: 甲南大学フロンティアサイエンス学部レクチャールーム、兵庫県
• [Presentation] Development of Novel Polyion Complex Vesicles, “PICsomes”, and Their Biomedical Applications
  • Author(s): A. Kishimura, Y. Anraku, S. Chuanoi, W. Kawamura, S. Kakiyama, K. Kataoka
  • Organizer: CIMTEC 2012 (4th International Conference on Smart Materials, Structures and Systems)
  • Place of Presentation: Palazzo dei Congressi, Montecatini Terme, Italy
• [Presentation] 粒径の制御された高分子ベシクルPICsomeの体内動態と生体内イメージング応用
  • Author(s): 岸村顕広、安楽泰孝、三浦裕、赤井淳、田中さやか、狩野光伸、国領大介、青木伊知男、小山博之、片岡一則
  • Organizer: 第28回日本DDS学会学術集会
  • Place of Presentation: 札幌コンベンションセンター、北海道
• [Presentation] 超常磁性酸化鉄微粒子を内包したポリイオンコンプレックス型中空ナノ粒子 PICsome を用いたin vivo MR イメージング
  • Author(s): 國領大介、安楽泰孝、岸村顕広、田中さやか、狩野光伸、佐賀恒夫、片岡一則、青木伊知男
  • Organizer: 第28回日本DDS学会学術集会
  • Place of Presentation: 札幌コンベンションセンター、北海道
• [Presentation] Development of Polyion Complex Vesicles “PICsomes” toward a Versatile Platform for Delivery of Biomacromolecules and Nano-materials
  • Author(s): A. Kishimura, Y. Anraku, S. Chuanoi, K. Miyata, D. Kokuryo, I. Aoki, T. Saga, K. Kataoka
  • Organizer: The 39th Annual Meeting & Exposition of the Controlled Release Society
  • Place of Presentation: Centre des congres de Que bec, Quebec City, Canada
• [Presentation] Development of Nano-sized Polyion Complex Vesicles “PICsomes” as a Tool for Investigation of in vivo Nano-environment
  • Author(s): A. Kishimura, Y. Anraku, K. Kataoka
  • Organizer: The 39th Annual Meeting & Exposition of the Controlled Release Society
  • Place of Presentation: Centre des congres de Que bec, Quebec City, Canada
• [Presentation] Devrlopment of Nano-sized Polyion Complex Vesicles (Nano-PICsome) for Versatile DDS Carriers
  • Author(s): Y. Anraku, A. Kishimura, K. Kataoka
  • Organizer: NanoBio Seattle 2012 (The Fourth International NanoBio Conference)
  • Place of Presentation: Bell Harbor International Convention Center, Seattle, USA
• [Presentation] Smart Multilayered Polymer/Silica Assemblies for siRNA Delivery
  • Author(s): K. Miyata, T. Suma, Y. Anraku, N. Nishiyama, K. Kataoka
  • Organizer: NanoBio Seattle 2012 (The Fourth International NanoBio Conference)
  • Place of Presentation: Bell Harbor International Convention Center, Seattle, USA
• [Presentation] Evaluation of size-controlled polyion complex hollow vesicles for abdominal aortic aneurysm imaging
  • Author(s): Y. Miura, A. Kishimura, Y. Anraku, A. Akai, H. Koyama, K. Kataoka
  • Organizer: NanoBio Seattle 2012 (The Fourth International NanoBio Conference)
  • Place of Presentation: Bell Harbor International Convention Center, Seattle, USA
• [Presentation] グアニジノ基を含有するポリカチオンを用いたポリイオンコンプレックス型ベシクル形成とその特性評価
  • Author(s): 堀真緒、安楽泰孝、岸村顕広、片岡一則
  • Organizer: 第61回高分子討論会
  • Place of Presentation: 名古屋工業大学、愛知県
• [Presentation] 疎水性薬剤を内包したポリイオンコンプレックス型ベシクルPICsomeの開発
  • Author(s): 笹野将人、安楽泰孝、岸村顕広、Cabral Horacio、西山伸宏、片岡一則
  • Organizer: 第61回高分子討論会
  • Place of Presentation: 名古屋工業大学、愛知県
• [Presentation] ポリイオンコンプレックス型ベシクルの電場応答挙動とその機構に関する考察
  • Author(s): 末吉大輝、小穴英廣、安楽泰孝、岸村顕広、鷲津正夫、片岡一則
  • Organizer: 第61回高分子討論会
  • Place of Presentation: 名古屋工業大学、愛知県
• [Presentation] Novel vesicles containing membrane-embedded siRNA as for nucleic acid therapeutics
  • Author(s): S. Chuanoi, A. Kishimura, K. Miyata, T. Suma, Y. Anraku, K. Kataoka
  • Organizer: IUMRS-ICEM2012
  • Place of Presentation: パシフィコ横浜、神奈川県
• [Presentation] The Effect of Chemical Structure in a Poly Ion Complex Vesicle layer on the Cellular Uptake
  • Author(s): S. Kakiyama, Y. Anraku, A. Kishimura, K. Kataoka
  • Organizer: IUMRS-ICEM2012
  • Place of Presentation: パシフィコ横浜、神奈川県
• [Presentation] Smart multilayered polymer/silica assemblies for enhanced siRNA delivery
  • Author(s): K. Miyata, T. Suma, S. Watanabe, Y. Anraku, N. Gouda, H. Takemoto, T. Ishii, A. Kishimura, N. Nishiyama, K. Kataoka
  • Organizer: IUMRS-ICEM2012
  • Place of Presentation: パシフィコ横浜、神奈川県
• [Presentation] Cellular Uptake of Polyion Complex Vesicles (PICsomes)
  • Author(s): S. Kakiyama, Y. Anraku, A. Kishimura, K. Kataoka
  • Organizer: UT-SNU-TJU Student Workshop
  • Place of Presentation: Tianjin University, Tianjin, China
• [Presentation] Development of Nano-sized Polyion Complex Vesicles (Nano-PICsomes) for Versatile DDS Carriers
  • Author(s): Y. Anraku, A. Kishimura, D. Kokuryo, S. Tanaka, M. Kamiya, K. Tou, T. Nomoto, Y. Matsumoto, I. Aoki, M. Kano, Y. Urano, K. Kataoka
  • Organizer: 2012 MRS Fall Meeting
  • Place of Presentation: Hynes Convention Center, Boston, Massachusetts, USA
• [Presentation] 表層にcRGDを導入したPICsomeの調製とその機能評価
  • Author(s): 川村渉、安楽泰孝、岸村顕広、片岡一則
  • Organizer: 第34回日本バイオマテリアル学会
  • Place of Presentation: 仙台国際センター、宮城県
• [Presentation] ポリイオンコンプレックス型ベシクルの化学特性に着目した細胞取込挙動の制御
  • Author(s): 柿山創、安楽泰孝、岸村顕広、片岡一則
  • Organizer: 第34回日本バイオマテリアル学会
  • Place of Presentation: 仙台国際センター、宮城県
• [Presentation] "Control of Polyion Complex Nano-architectures by Tuning Polymer Composition and Concentration "
  • Author(s): A. Wibowo, Y. Anraku, A. Kishimura, K. Kataoka
  • Organizer: SPSJ International Polymer Conference
  • Place of Presentation: Kobe Convention center, Hyogo, Japan
• [Presentation] Development of a versatile platform for macromolecule and nano-material delivery system based on polyion complex vesicles (PICsomes)
  • Author(s): A. Kishimura, Y. Anraku, D. Sueyoshi, K. Kataoka
  • Organizer: SPSJ International Polymer Conference
  • Place of Presentation: Kobe Convention center, Hyogo, Japan
• [Presentation] ソフト界面制御によるナノ・マイクロ粒子の微細構造制御とその機能開発
  • Author(s): 岸村顕広、A. Wibowo, S. Murugaboopathy, 安楽泰孝、末吉大輝、堀真緒、S. Chuanoi, 小穴英廣、鷲津正夫、片岡 一則
  • Organizer: "文部科学省・科学研究費補助金・新学術領域研究 「ソフトインターフェースの分子科学」第９回 公開シンポジウム"
  • Place of Presentation: タワーホール船堀、東京都
• [Presentation] PICsomeを用いた新規アクティブターゲッティングプラットフォームの構築
  • Author(s): 川村渉、安楽泰孝、岸村顕広、片岡一則
  • Organizer: 帝人21世紀フォーラム 2013
  • Place of Presentation: 帝人株式会社 富士教育研修所、静岡県
• [Presentation] Design of Novel Active Targeting Nano-Platform Based on Polyion Complex Vesicles “PICsomes”
  • Author(s): W. Kawamura, Y. Anraku, A. Kishimura, K. Kataoka
  • Organizer: 16th International Symposium on RECENT ADVANCES IN DRUG DELIVERY SYSTEMS
  • Place of Presentation: Sheraton Hotel, Salt Lake City, Utah, USA,
• [Presentation] Development of enzyme-loaded PICsomes as an in vivo reactor
  • Author(s): Y. Anraku
  • Organizer: Nanobio FIRST International Symposium between Sweden and Japan Achieving medical innovation by nanobio Bench to Bedside, Bedside to Bench
  • Place of Presentation: Ito Hall, Ito International Research Center, The University of Tokyo, Tokyo, Japan
• [Presentation] Photosensitizer Encapsulated Nanosized Polyion Complex Vesicles (Nano-PICsomes) for Enhanced In Vivo Photodynamic Therapy
  • Author(s): L. Xiao, H. Chen, Y. Anraku, H. Wu, X. Liu, H. Cabral, T. Nomoto, A. Kishimura, N. Nishiyama, K. Kataoka
  • Organizer: Nanobio FIRST International Symposium between Sweden and Japan Achieving medical innovation by nanobio Bench to Bedside, Bedside to Bench
  • Place of Presentation: Ito Hall, Ito International Research Center, The University of Tokyo, Tokyo, Japan
• [Presentation] Photosensitizer Encapsulated Nano Polyion Complex Vesicles (Nano-PICsome) for Enhanced In Vivo Photodynamic Therapy
  • Author(s): L. Xiao, H. Chen, Y. Anraku, X. Liu, H. Wu, H. Cabral, A. Kishimura, N. Nishiyama, K. Kataoka
  • Organizer: 2nd International Conference on Biomaterials Science in Tsukuba ICBS2013
  • Place of Presentation: Tsukuba International Congress Center (EPOCHAL Tsukuba), Tsukuba
• [Presentation] "Evaluation of responses of polyion complex vesicles (PICsomes) to Electric Fields"
  • Author(s): D. Sueyoshi, H. Oana, Y. Anraku, A. Kishimura, M. Washizu, K. Kataoka
  • Organizer: 2nd International Conference on Biomaterials Science in Tsukuba ICBS2013
  • Place of Presentation: Tsukuba International Congress Center (EPOCHAL Tsukuba), Tsukuba
• [Presentation] Stability Enhancement of Polyion Complex Vesicles (PICsomes) with Guanidinium-modified Polycations under the Physiological Condition
  • Author(s): M. Hori, Y. Anraku, A. Kishimura, K. Kataoka
  • Organizer: 2nd International Conference on Biomaterials Science in Tsukuba ICBS2013
  • Place of Presentation: Tsukuba International Congress Center (EPOCHAL Tsukuba), Tsukuba
• [Presentation] pH-triggered Morphological Changes of Polyion Complex Vesicles Based on Charge Conversion of Block Copolymers
  • Author(s): S. -G. Lee, Y. Anraku, A. Kishimura, K. Kataoka
  • Organizer: 2nd International Conference on Biomaterials Science in Tsukuba ICBS2013
  • Place of Presentation: Tsukuba International Congress Center (EPOCHAL Tsukuba), Tsukuba
• [Presentation] 低分子化合物のデリバリーを見据えたポリイオンコンプレックス型ベシクルの調製
  • Author(s): 後藤晃範、岸村顕広、安楽泰孝
  • Organizer: 日本薬学会 第133年会(横浜)「薬科学の底力」“Amazing Potential of Pharmaceutical Sciences: From Basics to Clinics.”
  • Place of Presentation: パシフィコ横浜、神奈川県
• [Presentation] SPIO-loaded unilamellar polyion complex vesicles (SPIO-Cy5-PICsomes) as a high relaxivity contrast agent for tumor detection
  • Author(s): D. Kokuryo, Y. Anraku, A. Kishimura, S. Tanaka, M. R. Kano, N. Nishiyama, T. Saga, I. Aoki, K. Kataoka
  • Organizer: International Society for Magnetic Resonance in Medicine, ISMRM
  • Place of Presentation: Salt Palace Convention Center, Solt lake city, Utah, USA
• [Remarks] 研究室ホームページ
  • URL: http://www.bmw.t.u-tokyo.ac.jp/index.html
• [Remarks] 片岡研究室ホームページ
  • URL: http://www.bmw.t.u-tokyo.ac.jp
• [Remarks] 東京大学大学院工学系研究科片岡研究室
  • URL: http://www.bmw.t.u-tokyo.ac.jp
• [Remarks] NanoBio FIRST
  • URL: http://park.itc.u-tokyo.ac.jp/nanobiof/index.html
• [Patent(Industrial Property Rights)] 物質充填静電結合型ベシクル (2013)
  • Inventor(s): 片岡一則、岸村顕広、安楽泰孝、後藤晃範
  • Industrial Property Rights Holder: 科学技術振興機構
  • Industrial Property Rights Type: 特許
  • Filing Date: 2013-03-01
• [Patent(Industrial Property Rights)] 物質充填静電結合型ベシクル (2012)
  • Inventor(s): 片岡一則、岸村顕広、安楽泰孝
  • Industrial Property Rights Holder: 片岡一則、岸村顕広、安楽泰孝
  • Industrial Property Rights Type: 特許
  • Filing Date: 2012-12-10