Early detection of disease using surface enhance Raman scattering (SERS)-dynamic gold nanoparticle to detect inflammatory response in cells

• Project/Area Number: 24700481
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Osaka University
• Principal Investigator: PISSUWAN Dakrong 大阪大学, 免疫学フロンティア研究センター, 特任研究員(常勤) (40622147)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 金ナノ粒子 / SERS / 炎症反応 / ICAM-1 / サイトカイン

Research Abstract

The investigation of the use of surface enhanced Raman scattering (SERS) gold nanoparticles for rapid monitoring of inflammatory responses at the early stage of diseases. Gold nanorods (GNRs) combined with Raman probes (4-mercaptobenzoic acid) are conjugated with intercellular adhesion molecule-1 antibody (anti-ICAM-1). The prepared particles could be used as a SERS probe to detect the expression of ICAM-1 in macrophages treated lipopolysaccharide for 1 h which is faster than using ELISA or fluorescent labeling techniques.

Research Products

• [Journal Article] Distribution of label free cationic polymer-coated gold nanorods in live macrophage cells reveals formation of groups of intracellular SERS signals of probe nanoparticles (2014)
  • Author(s): Pissuwan, D., Hobro, A., Pavillon, N., Smith, N.
  • Journal Title: RSC Advances Volume: Vol.4 Pages: 5536-5541
• [Journal Article] Distribution of label free cationic polymer-coated gold nanorods in live macrophage cells reveals formation of groups of intracellular SERS signals of probe nanoparticles (2014)
  • Author(s): Pissuwan, D., Hobro, A., Pavillon, N., Smith, N.
  • Journal Title: RSC Adv. Volume: 4 Issue: 5 Pages: 5536-5541
  • DOI: 10.1002/ppsc.201200115
  • Peer Reviewed
• [Journal Article] Effect of surface-modified gold nanorods on the inflammatory cytokine response in macrophage cells (2013)
  • Author(s): Pissuwan, D., Kumagai, Y., Smith, N.
  • Journal Title: Particle and Particle System Characterization Volume: Vol.30 Pages: 427-433
• [Journal Article] Biodistribution and tumor localization of PEG-modified dendritic poly(L-lysine) oligonucleotide complexes (2013)
  • Author(s): R. Kurihara, D. Pissuwan, T. Mori, Y. Katayama, T. Niidome
  • Journal Title: J. Biomater. Sci.-Polym. Ed. Volume: 23 Issue: 18 Pages: 2369-2380
  • DOI: 10.1163/156856211x617722
  • Peer Reviewed
• [Journal Article] Gold nanorods in an oil-base formulation for transdermal treatment of type 1 diabetes in mice (2012)
  • Author(s): K. Nose, D. Pissuwan, M. Goto Y. Katayama and T. Niidome
  • Journal Title: Nanoscale Volume: 4 Issue: 12 Pages: 3776-3780
  • DOI: 10.1039/c2nr30651d
  • Peer Reviewed
• [Presentation] The uptake and intracellular signal monitoring in macrophage cells of different surface modified gold nanorods using surface enhanced Raman scattering spectroscopy (2013)
  • Author(s): Pissuwan, D., Hobro, A., Pavillon, N., Smith, N.
  • Organizer: 7th International Conference on Advanced Vibrational Spectroscopy (ICAVS)
  • Place of Presentation: Kobe, Japan
• [Presentation] Gold nanorods : Tool to detect early stage inflammation and their surface effects on inflammatory response in macrophage cells (2013)
  • Author(s): Pissuwan, D., Smith, N.
  • Organizer: The 4th International Nanomedicine Conference
  • Place of Presentation: Sydney, Australia
• [Presentation] The uptake and intracellular signal monitoring in macrophage cells of different surface modified gold nanorods using surface-enhanced Raman scattering spectroscopy (2013)
  • Author(s): Pissuwan, D., Hobro, A., Pavillon, N., Smith, N.
  • Organizer: Seventh International Conference on Advanced Vibrational Spectroscopy (ICAVs7)
  • Place of Presentation: Kobe Convention Center, Kobe, Japan
• [Presentation] Gold nanorods: Tool to detect early-stage inflammation and their surface effects on inflammatory response in macrophage cells (2013)
  • Author(s): Pissuwan, D., Smith, N.
  • Organizer: The 4th International Nanomedicine Conference Sydney
  • Place of Presentation: Intercontinental Hotel Sydney, Asutralia
• [Presentation] Intersection of light and gold nanoparticles for biomedical applications (2012)
  • Author(s): Pissuwan, D., Cortie, M., Niidome, T., Smith, N.
  • Organizer: Photonics Global Conference
  • Place of Presentation: Singapore
  • Invited
• [Presentation] Intersection of light and gold nanoparticles for biomedical applications (2012)
  • Author(s): Pissuwan, D.
  • Organizer: Photonics Global Conference
  • Place of Presentation: Resort World Convention Centre, Singapore
  • Invited
• [Presentation] Effect of different surface modifications of gold nanorods on inflammatory cytokine induction in macrophage cells (2012)
  • Author(s): Pissuwan, D., Smith, N.
  • Organizer: Material Research Society (MRS)
  • Place of Presentation: Hynes Convention Center, Boston, USA
• [Presentation] Transdermal insulin delivery enhanced by phptothermal effect of gold nanorods (2012)
  • Author(s): Pissuwan, D., Nose, K., Mori, T., Katayama, Y., Niidome, T.
  • Organizer: Controlled Release Society (CRS) Meeting
  • Place of Presentation: Centre des congress de Quebec, Quebec City, Canada
• [Book] Gold nanoparticles for the development of transdermal delivery system, Functional Nanoparticles for Bioanalysis, Nanomedicine, and Bioelectronic Devices Volume 2 (2012)
  • Author(s): Pissuwan, D., Niidome, T.
  • Publisher: ACS Publication
• [Remarks]
  • URL: http://www.dakrong.net