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Development of biomolecule separation system by hybrid coacervate using hydraulic pressure

Research Project

Project/Area Number 24700483
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biomedical engineering/Biological material science
Research InstitutionKagoshima University

Principal Investigator

KAZUYA YAMAMOTO  鹿児島大学, 理工学研究科, 助教 (40347084)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywordsインテリジェント材料 / コアセルベート / 高分子合成
Research Abstract

We examined the development of stimuli-responsive hybrid coacervate as the reaction field under high pressure condition by hydraulic pressure. First, we designed the acrylamide-based copolymers with higher molecular weight randomly containing carboxyl or hydroxyl groups. Their stimuli-responsive behavior on comonomer composition and pH of the polymer solution was precisely investigated. The coacervate droplets were easily formed above a lower critical solution temperature. Furthermore, we studied the coacervate droplet formation under high pressure conditions. Next, we examined radical polymerization and biomolecule separation system with the coacervate droplet under high pressure. When we added the ferritin protein cages (ca. 12 nm) as model biomolecule to the coacervate droplets, the composite emulsions were stable for a long time. Furthermore, we examined the assembly behavior of ferritin on the liquid-liquid and liquid-solid interface.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (10 results)

All 2014 2013 2012

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (6 results)

  • [Journal Article] Yamashita Solid-phase PEGylation of an Immobilized Protein Cage on Polyelectrolyte Multilayer Colloids and Surfaces B2014

    • Author(s)
      K. Uto, K. Yamamoto, K. Iwahori, T. Aoyagi, I.
    • Journal Title

      Biointerfaces

      Volume: 113 Pages: 338-345

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Solid-phase PEGylation of an immobilized protein cage on polyelectrolyte multilayer2014

    • Author(s)
      K. Uto, K. Yamamoto, K. Iwahori, T. Aoyagi, I. Yamashita.
    • Journal Title

      Colloids and Surfaces B: Biointerfaces

      Volume: 113 Pages: 338-345

    • DOI

      10.1016/j.colsurfb.2013.09.013

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Yamashita Characterization of Stable, Electroactive Protein Cage/Synthetic Polymer Multilayer Thin Films Prepared by Layer-by-layer Assembly2013

    • Author(s)
      K. Uto, K. Yamamoto, N. Kishimoto, M. Muraoka, T. Aoyagi, I.
    • Journal Title

      Journal of Nanoparticle Research

      Volume: 15 Pages: 1516-1526

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of Stable, Electroactive Protein Cage/Synthetic Polymer Multilayer Thin Films Prepared by Layer-by-layer Assembly2013

    • Author(s)
      K. Uto, K. Yamamoto, N. Kishimoto, M. Muraoka, T. Aoyagi, I. Yamashita
    • Journal Title

      Journal of Nanoparticle Research

      Volume: 15 Issue: 4 Pages: 1516-1526

    • DOI

      10.1007/s11051-013-1516-6

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] Atom Transfer Radical Polymerization of N-Isopropylacrylamide under High Pressure Conditions2012

    • Author(s)
      Yuji Hoshino, Kazuya Yamamoto, and Junichi Kadokawa
    • Organizer
      Japan-Taiwan Bilateral Workshop on Nanoascience
    • Place of Presentation
      Osaka university
    • Year and Date
      2012-12-05
    • Related Report
      2013 Final Research Report
  • [Presentation] 高圧条件下でのATRPにより調製されたポリ(N -イソプロピルアクリミド)の構造および溶液挙動評価2012

    • Author(s)
      星野裕志、山元和哉、門川淳一
    • Organizer
      日本化学会西日本大会
    • Place of Presentation
      佐賀大学
    • Year and Date
      2012-11-11
    • Related Report
      2013 Final Research Report
  • [Presentation] 高圧条件下でのN-イソプロピルアクリルアミドの原子移動ラジカル重合2012

    • Author(s)
      星野裕志、山元和哉、門川淳一
    • Organizer
      第49回化学関連支部合同九州大会
    • Place of Presentation
      北九州国際会議場
    • Year and Date
      2012-06-30
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] 高圧力条件下で調製したポリ(N-イソプロピルアクリルアミド)の溶液挙動2012

    • Author(s)
      星野裕志、山元和哉、門川淳一
    • Organizer
      第61回高分子学会年次大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2012-05-30
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] 高圧条件下での ATRP により調製されたポリ (N -イソプロピルアクリミド )の構造および溶液挙動評価2012

    • Author(s)
      星野裕志、山元和哉、門川淳一
    • Organizer
      日本化学会西日本大会
    • Place of Presentation
      佐賀大学
    • Related Report
      2012 Research-status Report
  • [Presentation] Atom Transfer Radical Polymerization of N-Isopropylacrylamide under High Pressure Conditions2012

    • Author(s)
      Yuji Hoshino, Kazuya Yamamoto, and Jun-ichi Kadokawa
    • Organizer
      Japan-Taiwan Bilateral Workshop on Nanoascience
    • Place of Presentation
      Osaka University
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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