Development of methods for cell reprogramming with a persistent RNA vector for regenerative medicine
| Project/Area Number |
24700490
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
SANO Masayuki 独立行政法人産業技術総合研究所, 幹細胞工学研究センター, 主任研究員 (80415687)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 遺伝子デリバリー / 細胞リプログラミング / 機能性RNA / SeVdpベクター / 遺伝子発現制御 / iPS細胞 / マイクロRNA |
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| Research Abstract |
To generate transgene-free cells by cell reprogramming using a persistent RNA vector based on a Sendai virus mutant Cl.151, it is important to eliminate the genome of Sendai virus vector from infected cells. For a wide range of application of cell reprogramming, we examined methods to eliminate the vector genome using an artificial riboswitch or microRNAs, which could inhibit the gene expression by addition of small molecules or in a tissue-specific manner.
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Report
(3 results)
Research Products
(14 results)
