| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
To generate transgene-free cells by cell reprogramming using a persistent RNA vector based on a Sendai virus mutant Cl.151, it is important to eliminate the genome of Sendai virus vector from infected cells. For a wide range of application of cell reprogramming, we examined methods to eliminate the vector genome using an artificial riboswitch or microRNAs, which could inhibit the gene expression by addition of small molecules or in a tissue-specific manner.
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