| Project/Area Number |
24700499
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical systems
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| Research Institution | National Center for Geriatrics and Gerontology (2013)
Kumamoto University (2012) |
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Principal Investigator |
KEISUKE Abe 独立行政法人国立長寿医療研究センター, 再生再建医学研究部, 流動研究員 (10535652)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | nsPEF / apoptosis / ceramide / p53 / ROS / IBSEF / ナノ秒パルス高電界 / バースト正弦波高電界 / signal pathway / USA / EU |
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| Research Abstract |
In this research, the apoptosis induction mechanism to the cancer cell by a nanosecond pulse electric field (nsPEF) was clarified in detail. In HeLaS3 cell of p53 inactivity, Ca2+ inflow occurs by nsPEF stimulus, and CERAMIDE is formed in a cell membrane neighboring part. And ROS is produced inside a cell, the apoptosis pathway of endoplasmic-reticulum-stress is caused, and initiate apoptosis. Furthermore, in cell and MCF7 and A549 of p53 activated form, nsPEF activates Bax and the apoptosis of a mitochondrial pathway is caused. As a result, nsPEF could induced apoptosis in all cancer cells. Now, I am developing the low-stress transgenics experiment and try the animal experiment.
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