| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Outline of Final Research Achievements |
We developed a new cryopreservation method where living organisms to be frozen are suspended in silicon oil. When processed in suspension in this manner, living organisms in a 100-micrometer-order size, for example, were found to be maintained in a supercooled state down to nearly -20°C. Furthermore, this suspended processing followed by slow freezing tended to increase the survival rate compared to the processing without slow freezing. Besides, as a new cryopreservation method, we also developed a device for freezing living organisms as solid particles in an approximate minimum size of 100 micrometer.
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