Development of ultrasound-induced phonophoresis to promote scar healing

• Project/Area Number: 24700530
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Rehabilitation science/Welfare engineering
• Research Institution: Kobe University
• Principal Investigator: Maeshige Noriaki 神戸大学, 保健学研究科, 助教 (90617838)
• Research Collaborator: TORII Kazuhiro ; TABUCHI Hiroto ; SAKA Ayumi ; ARAKI Kyoko ; KOGA Yuka ; OCHI Akane ; UEMURA Mikiko
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 超音波 / 短鎖脂肪酸 / 線維化 / 線維芽細胞 / 瘢痕 / 超音波療法 / 脂肪酸 / ソノポレーション / フォノフォレーシス

Outline of Final Research Achievements

We elucidated the ultrasound parameters to promote the antifibrogenic and anti-inflammatory effects by short chain fatty acids. Butyrate is a fatty acid having the most potent physiological action among short chain fatty acids. We found the antifibrogenic effects of butyrate in human dermal fibroblasts, keloid fibroblasts and pterygium fibroblasts, and facilitatory effects of low-intensity ultrasound (0.1-0.5 W/cm2) for the antifibrogenic and anti-inflammatory effects by butyrate. In addition, we found the promotional effects of low-intensity ultrasound on histone acetylation by a low-concentration of butyrate.

Research Products

• [Journal Article] 短鎖脂肪酸の線維化促進因子発現抑制に対する超音波照射の促進作用 (2014)
  • Author(s): 前重 伯壮; 田淵 寛人; 古賀 由華; 越智 茜; 植村 弥希子; 石川 （青山） 倫子; 三好 真琴; 杉元 雅晴; 寺師 浩人; 宇佐美 眞
  • Journal Title: 物理療法科学 Volume: 21 Pages: 53-58
  • NAID: 40020282870
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 短鎖脂肪酸の線維化促進因子発現抑制に対する超音波照射の促進作用 (2014)
  • Author(s): 前重伯壮、田淵寛人、古賀由華、越智茜、植村弥希子、石川（青山）倫子、三好真琴、杉元雅晴、寺師浩人、宇佐美眞
  • Journal Title: 日本物理療法学会会誌 Volume: 21
  • NAID: 40020282870
  • Peer Reviewed
• [Journal Article] Anti-fibrogenic effects of short chain fatty acids and ω-3 polyunsaturated fatty acid on keloid fibroblasts. (2014)
  • Author(s): Kazuhiro Torii; Noriaki Maeshige; Michiko Aoyama; Midori Imai; Hiroto Tabuchi; Makoto Miyoshi; Yasuhiro Hamada; Hiroto Terashi; Makoto Usami
  • Journal Title: Wound Repair and Regeneration Volume: 22
• [Journal Article] 脂質研究の最近の話題‐創傷治癒と脂肪酸代謝 (2013)
  • Author(s): 寺師浩人、前重伯壮、野村正、宇佐美眞
  • Journal Title: 静脈経腸栄養 Volume: 28 Pages: 939-943
• [Journal Article] 超音波の照射時間率がヒト皮膚由来線維芽細胞のα-SMA発現に対して与える影響 (2012)
  • Author(s): 前重伯壮、鳥井一宏、田淵寛人、青山倫子、寺師浩人、杉元雅晴、宇佐美眞
  • Journal Title: 日本物理療法学会誌 Volume: 19 Pages: 44-48
  • NAID: 40019430394
  • Peer Reviewed
• [Journal Article] 1ページ講座 「理学療法関連用語」 8.瘢痕． (2012)
  • Author(s): 前重伯壮
  • Journal Title: 理学療法ジャーナル Volume: 46 Pages: 725-725
• [Presentation] Butyrate and phenylbutyrate suppress fibrosis in human pterygium fibroblasts (2015)
  • Author(s): Yuka Koga; Noriaki Maeshige; Mikiko Uemura; Michiko Aoyama-Ishikawa; Motoki Kai; Yuya Nishiyama; Makoto Miyoshi; Chikako Katakami; Makoto Usami
  • Organizer: The 16th Congress of Parenteral and Enteral Nutrition Society of Asia
  • Place of Presentation: Nagoya (Japan)
  • Year and Date: 2015-07-24
  • Int'l Joint Research
• [Presentation] The effects of butyrate/DHA on apoptosis and apoptosis related factors in keloid fibroblasts (2015)
  • Author(s): Noriaki Maeshige; Akane Ochi; Yuka Koga; Mikiko Uemura; Michiko Aoyama-Ishikawa; Hayato Yamashita; Makoto Miyoshi; Taketo Inoue; Mayu Fujiwara; Motoki Kai; Yuya Nishiyama; Hiroto Terashi; Makoto Usami
  • Organizer: 12th Asian Congress of Nutrition
  • Place of Presentation: パシフィコ横浜（横浜）
  • Year and Date: 2015-05-14 – 2015-05-18
• [Presentation] Anti-inflammatory and antifibrogenic effects of butyrate/DHA on lipopolysaccharide stimulated dermal fibroblasts (2015)
  • Author(s): Yuka Koga; Noriaki Maeshige; Michiko Aoyama-Ishikawa; Akane Ochi; Mikiko Uemura; Motoki Kai; Yuya Nishiyama; Makoto Miyoshi; Michiko Takahashi; Hiroto Terashi; Makoto Usami
  • Organizer: 12th Asian Congress of Nutrition
  • Place of Presentation: Yokohama (Japan)
  • Year and Date: 2015-05-14
  • Int'l Joint Research
• [Presentation] The effects of butyrate/DHA on apoptosis and apoptosis related factors in keloid fibroblasts (2015)
  • Author(s): Noriaki Maeshige; Aakane Ochi; Yuka Koga; Mikiko Uemura; Michiko Aoyama-Ishikawa; Hayato Yamashita; Makoto Miyoshi; Taketo Inoue; Mayu Fujiwara; Motoki Kai; Yuya Nishiyama; Hiroto Terashi; Makoto Usami
  • Organizer: 12th Asian Congress of Nutrition
  • Place of Presentation: Yokohama (Japan)
  • Year and Date: 2015-05-14
  • Int'l Joint Research
• [Presentation] Low-intensity Ultrasound Enhances Histone Acetylation and Improves Anti-inflammatory Effects by Short Chain Fatty Acid Treatments in Human Dermal Fibroblasts (2015)
  • Author(s): Noriaki Maeshige; Yuka Koga; Junya Takegaki; Masayuki Tanaka; Michiko Aoyama-Ishikawa; Makoto Miyoshi; Makoto Usami; Hidemi Fujino
  • Organizer: Experimental Biology 2015
  • Place of Presentation: Boston Convention & Exhibition Center(ボストン、アメリカ合衆国)
  • Year and Date: 2015-04-01 – 2015-04-02
• [Presentation] Low-intensity ultrasound enhances histone acetylation and improves anti-inflammatory effects by short chain fatty acid treatments in human dermal fibroblasts (2015)
  • Author(s): Noriaki Maeshige; Yuka Koga; Junya Takegaki; Masayuki Tanaka; Michiko Aoyama-Ishikawa; Makoto Miyoshi; Makoto Usami; Hidemi Fujino
  • Organizer: Experimental Biology 2015
  • Place of Presentation: Boston (USA)
  • Year and Date: 2015-04-01
  • Int'l Joint Research
• [Presentation] ケロイド線維芽細胞に対する酪酸・DHA の apoptosis 誘導効果 (2014)
  • Author(s): 植村 弥希子; 前重 伯壮; 越智 茜; 古賀 由華; 青山 倫子; 山下 勇人; 三好 真琴; 井上 岳人; 寺師 浩人; 宇佐美 眞
  • Organizer: 第44回日本創傷治癒学会
  • Place of Presentation: ホテルメトロポリタン仙台（仙台）
  • Year and Date: 2014-12-02 – 2014-12-03
• [Presentation] 短鎖脂肪酸の抗線維化作用に対する超音波照射の促進効果‐in vitro実験による検証‐ (2013)
  • Author(s): 前重伯壮、田淵寛人、古賀由華、越智茜、植村弥希子、石川(青山)倫子、三好真琴、杉元雅晴、寺師浩人、宇佐美眞
  • Organizer: 第21回日本物理療法学会学術大会
  • Place of Presentation: 横須賀
• [Presentation] Antifibrogenic and proapoptotic effects of butyrate/DHA via PGE2 secretion on keloid fibroblasts in inflammatory model. (2013)
  • Author(s): Maeshige Noriaki; Torii Kazuhiro; Tabuchi Hiroto; Saka Ayumi; Araki Kyoko; Ishikawa Aoyama Michiko; Imai Midori; Koga Yuka; Ochi Akane; Uemura Mikiko; Miyoshi Makoto; Hamada Yasuhiro; Terashi Hiroto; Usami Makoto
  • Organizer: The 35th congress of the European Society of Parenteral and Enteral Nutrition
  • Place of Presentation: Leipzig（ドイツ）
• [Presentation] 酪酸・DHA併用がケロイド由来線維芽細胞における α-SMA、collagen の発現に与える抑制効果
  • Author(s): 田淵寛人、前重伯壮、鳥井一宏、青山倫子、今井緑、三好真琴、小谷穣治、濱田康弘、寺師浩人、宇佐美眞
  • Organizer: 第19回外科侵襲とサイトカイン研究会
  • Place of Presentation: 神戸
• [Book] 実学としての理学療法概観－物理療法学－ (2015)
  • Author(s): 前重 伯壮
  • Publisher: 文光堂
• [Remarks] 神戸大学医学部保健学科病態代謝学外科代謝栄養学研究室
  • URL: http://www.research.kobe-u.ac.jp/fhs-usami/index.html
• [Remarks] 神戸大学大学院保健学研究科病態代謝学外科代謝栄養学研究室ホームページ
  • URL: http://www.research.kobe-u.ac.jp/fhs-usami/member.html
• [Remarks] 神戸大学医学部保健学科病態代謝学外科代謝栄養学研究室ホームページ
  • URL: http://www.research.kobe-u.ac.jp/fhs-usami/index.html