Proteomic analysis of nitrated proteins in the skeletal muscle after physical exercise.

• Project/Area Number: 24700761
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied health science
• Research Institution: Juntendo University
• Principal Investigator: UDA Munehiro 順天堂大学, 医学(系)研究科(研究院), 研究員 (80549262)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: ニトロトリプトファン / 高強度間欠性走運動 / 細胞内シグナル伝達タンパク質 / プロテオーム解析 / ニトロ化修飾 / シグナル伝達 / 骨格筋 / 身体運動 / トリプトファン残基 / チロシン残基

Research Abstract

We identified nitrotyptophan-containing proteins from adult rat skeletal muscle under physiological condition. The degree of typtophan nitration in the skeletal muscle were not changed by high intensity intermittent running. Thus, nitrotyptophan-containing proteins under physiological condition may not function as a scavenger of reactive oxygen and nitrogen species. In addition, the typtophan nitration of alpha-skeletal muscle actin, which is detected at approximately 60 kDa, and ERK that is involved in the signal transduction in cells were not changed by high intensity intermittent running.

Research Products

• [Journal Article] Proteomic analysis of endogenous nitrotryptophan-containing proteins in rat hippocampus and cerebellum (2012)
  • Author(s): Uda M, Kawasaki H, Shigenaga A, Baba T, Yamakura F
  • Journal Title: Bioscience reports Volume: 32 Issue: 6 Pages: 521-530
  • DOI: 10.1042/bsr20120032
  • Peer Reviewed
• [Presentation] Presence of nitrotryptophan-containing proteins in the adult rat skeletal muscle (2013)
  • Author(s): Munehiro Uda,Hiroaki Kawasak,kyoichi Iizumi,Ayako Shigenaga,Takeshi Baba,and Fumiyuki Yamakura
  • Organizer: 20th Annual Meeting of the Society for Free Radcal Biology and Medicine
  • Place of Presentation: San Antonio,TX,U. S. A
  • Year and Date: 2013-11-22
• [Presentation] Presence of nitrotryptophan-containing proteins in the adult rat skeletal muscle (2013)
  • Author(s): Munehiro Uda, Hiroaki Kawasaki, Kyoichi Iizumi, Ayako Shigenaga, Takeshi Baba, and Fumiyuki Yamakura
  • Organizer: 20th annual meeting of the society for free radical biology and medicine
  • Place of Presentation: Grand hyatt san antonio, San antonio, TX
• [Presentation] Proteomic analysis of endogenous nitrotryptophan-containing proteins in rat hippocampus and cerebellum (2012)
  • Author(s): MunehiroUda、HiroakiKawasaki、Ayako Shigenaga、Takeshi Baba、Fumiyuki Yamakura
  • Organizer: 13th International Society for Tryptophan Research
  • Place of Presentation: Sydney,Australia
  • Year and Date: 2012-11-08
• [Presentation] 骨格筋におけるニトロトリプトファン含有タンパク質のプロテオーム解析 (2012)
  • Author(s): 宇田宗弘、川崎広明、重永綾子、馬場猛、山倉文幸
  • Organizer: 第67回日本体力医学会大会
  • Place of Presentation: 岐阜県
  • Year and Date: 2012-09-15
• [Presentation] 骨格筋におけるニトロトリプトファン含有タンパク質のプロテオーム解析 (2012)
  • Author(s): 宇田 宗弘、川崎 広明、重永 綾子、馬場 猛、山倉 文幸
  • Organizer: 第67回日本体力医学会大会
  • Place of Presentation: 長良川国際会議場 ・ 岐阜都ホテル ( 岐阜県 )
• [Presentation] Proteomic analysis of endogenous nitrotryptophan-containing proteins in rat hippocampus and cerebellum. (2012)
  • Author(s): Munehiro Uda, Hiroaki Kawasaki, Ayako Shigenaga, Takeshi Baba, Fumiyuki Yamakura
  • Organizer: 13th International Society for Tryptophan Research
  • Place of Presentation: Sydney Masonic Centre ( Sydney, Australia )
• [Presentation] Proteomic and functional analysis of protein tryptophan nitration in the neuronal cell differentiation. (2012)
  • Author(s): Hiroaki Kawasaki, Ayako Shigenaga, Takeshi Baba, Keiichi Ikeda, Munehiro Uda, Kenji Takamori, Fumiyuki Yamakura
  • Organizer: 13th International Society for Tryptophan Research
  • Place of Presentation: Sydney Masonic Centre ( Sydney, Australia )