Proteomic analysis of nitrated proteins in the skeletal muscle after physical exercise.
Project/Area Number |
24700761
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Applied health science
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Research Institution | Juntendo University |
Principal Investigator |
UDA Munehiro 順天堂大学, 医学(系)研究科(研究院), 研究員 (80549262)
|
Project Period (FY) |
2012-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | ニトロトリプトファン / 高強度間欠性走運動 / 細胞内シグナル伝達タンパク質 / プロテオーム解析 / ニトロ化修飾 / シグナル伝達 / 骨格筋 / 身体運動 / トリプトファン残基 / チロシン残基 |
Research Abstract |
We identified nitrotyptophan-containing proteins from adult rat skeletal muscle under physiological condition. The degree of typtophan nitration in the skeletal muscle were not changed by high intensity intermittent running. Thus, nitrotyptophan-containing proteins under physiological condition may not function as a scavenger of reactive oxygen and nitrogen species. In addition, the typtophan nitration of alpha-skeletal muscle actin, which is detected at approximately 60 kDa, and ERK that is involved in the signal transduction in cells were not changed by high intensity intermittent running.
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Report
(3 results)
Research Products
(8 results)