Prevention of photoaging by scavenging mitochondrial singlet oxygen
| Project/Area Number |
24700762
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied health science
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| Research Institution | Nippon Medical School |
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Principal Investigator |
WOLF Alexander 日本医科大学, 付置研究所, 講師 (20434136)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 光老化 / 老化 / 酸化ストレス / 皮膚 / skin / oxidative stress / aging / GFP |
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| Research Abstract |
Due to a high desire to look young and beautiful, cosmetics to slow down aging are a large market. Wrinkle formation has been attributed to UVA radiation.
Using hairless transgenic mice expressing a redox-sensitive GFP (roGFP1) in the cytosol and in mitochondria of epidermal keratinocytese, we discovered that UVA induces oxidative stress in skin mitochondria but not cytosol. We used in vivo oxidative stress imaging to evaluate singlet oxygen scavengers. Topical application of carotenoids did not prevent UVA-induced oxidative stress. We assume this was due to poor permeation of the large carotenoids across the stratum corneum. However, a low molecular weight compound able to permeate the stratum corneum well could reduce the amount of oxidation induced by UVA relative to vehicle control.
Future research will further investigate substances that can prevent UVA or visible light-induced oxidative stress by topical application to skin.
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Report
(3 results)
Research Products
(12 results)
