| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
MC3T3-E1 cells were attached on biodegradable polymer scaffold and then cultured for 3 days. To investigate the expression profiles of miRNAs in hydrostatic pressure-stimulated MC3T3-E1 cells, we subjected their total RNA extracted from hydrostatic pressure-stimulated (1.5 MPa for 1 h) or nontreated cells to miRNA array chips. The expression levels of 120 miRNAs (log ratio 3 <) were distinct between hydrostatic pressure-stimulated and nontreated cells. Among them, we focused on the 3 kinds of down-regulated miRNAs, because the nucleotide sequences of these miRNAs were similar and the semaphorin 3A that is closely related in osteoblast differentiation was a common target gene for each miRNAs. The enforced expression of these mature miRNAs in MC3T3-E1 cells remarkably attenuated a hydrostatic pressure-stimulated preosteoblast differentiation. From these results, it was presumed that those miRNAs act as important factors for regulatory machinery involved in early osteogenesis in part.
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