Project/Area Number |
24700810
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Eating habits, studies on eating habits
|
Research Institution | Nagoya University of Arts and Sciences |
Principal Investigator |
MASAKI Takeshi 名古屋学芸大学, 管理栄養学部, 講師 (10387912)
|
Project Period (FY) |
2012-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | アレルギー・ぜんそく / 蛋白質 / 酵素 / 植物 / 食品 / ダイズ / 発芽 / プロテアーゼ / ダイズ(大豆) / 種子貯蔵タンパク質 / アレルゲン / 酵素タンパク質 |
Research Abstract |
We revealed that three major allergic proteins (alpha-subunit of beta-conglycinin, Gly m Bd 28K, Gly m Bd 30K) of soybean decrease remarkably under soybean seed germination or soybean seedling growth. Although alpha-subunit of beta-conglycinin and Gly m Bd 28K have disappeared until 5days after imbibition, Gly m Bd 30K started to decrease since 14days after imbibition. It shows that Gly m Bd 30 K is degraded by another protease from that degrade alpha-subunit of beta-conglycinin and Gly m Bd 28K. In addition, Gly m Bd 28K in solution prepared from soybean seedlings decreased when its solution was incubated. Such in vitro decrease of Gly m Bd 28 K was disturbed by addition of aspartic protease inhibitor. It indicates that some kind of aspartic protease degrade Gly m Bd 28K.
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