Global analysis of the lipophilic reactive carbonyls present in biological specimens by LC/ESI-MS/MS

• Project/Area Number: 24700838
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Eating habits, studies on eating habits
• Research Institution: University of Shizuoka
• Principal Investigator: TOMONO Susumu 静岡県立大学, 食品栄養科学部, 客員共同研究員 (60554011)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: アルデヒド / カルボニル / LC/MS / 脂質過酸化 / 酸化ストレス / LC-MS / 脂質過酸化物 / 分析科学

Outline of Final Research Achievements

Toxic aldehydes and ketones (that is, reactive carbonyl species; RCs) have been suspected of causing cardiovascular disease, neurodegenerative disease and other chronic diseases. In the present study, we have developed a highly sensitive and selective method that allowed us to detect numerous lipophilic RCs simultaneously. A new analytical method has been developed for profiling lipophilic RCs in biological samples using liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) with selected reaction monitoring (SRM). The method was found to be sensitive, reproducible, accurate and specific for RCs. Our novel approach could be very useful for establishing the RCs profiles of biological and environmental samples, as well as studying the roles of lipid peroxidation in oxidative stress related-disorders and discovering new biomarkers for the early diagnosis of these diseases.

Research Products

• [Journal Article] Comprehensive analysis of the lipophilic reactive carbonyls present in biological specimens by LC/ESI-MS/MS. (2015)
  • Author(s): Susumu Tomono, Noriyuki Miyoshi, Hiroshi Ohshima
  • Journal Title: Journal of Chromatography B Volume: 988 Pages: 149-156
  • DOI: 10.1016/j.jchromb.2015.02.036
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Implications of cholesterol autoxidation products in the pathogenesis of inflammatory diseases (2014)
  • Author(s): Miyoshi N, Iuliano L, Tomono S, Ohshima H.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 446 Issue: 3 Pages: 702-708
  • DOI: 10.1016/j.bbrc.2013.12.107
  • Peer Reviewed
• [Journal Article] Cytotoxic Effects of Secosterols and Their Derivatives on Several Cultured Cells (2013)
  • Author(s): Tomono S
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 77 Issue: 3 Pages: 651-653
  • DOI: 10.1271/bbb.120758
  • NAID: 10031164790
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Occurrence of cytotoxic 9-oxononanoyl secosterol aldehydes in human low density lipoprotein. (2013)
  • Author(s): Miyoshi N, Iwasaki N, Tomono S, Higashi T, Ohshima H.
  • Journal Title: Free Radic.Biol.Med. Volume: 60 Pages: 73-79
  • DOI: 10.1016/j.freeradbiomed.2013.01.029
  • Peer Reviewed
• [Journal Article] Development and application of a method for identification of isothiocyanate-targeted molecules in colon cancer cells (2012)
  • Author(s): Miyoshi, N., Yonemochi, T., Tomono, S., Fukutomi, R., Nakamura, Y., Ohshima, H.
  • Journal Title: Anal. Biochem. Volume: 429(2) Issue: 2 Pages: 124-131
  • DOI: 10.1016/j.ab.2012.07.018
  • Peer Reviewed
• [Journal Article] Dysregulation of dimethylargininedimethylaminohydrolase/asymmetric dimethylarginine pathway in rat type II diabetic nephropathy (2012)
  • Author(s): Miyoshi N
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 51 Issue: 2 Pages: 143-149
  • DOI: 10.3164/jcbn.11-33
  • NAID: 130004879298
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed
• [Presentation] 多層カーボンナノチューブ肺内投与によって生じる活性カルボニル化合物の網羅的解析 (2014)
  • Author(s): 伴野勧、三好規之、徐結苟、津田洋幸、大島寛史
  • Organizer: 第67回日本酸化ストレス学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2014-09-04 – 2014-09-05
• [Presentation] Global analysis of reactive carbonyls formed in neuronal PC-12 cells by exposed to amyloid-beta (2014)
  • Author(s): Tomono S, Nagasawa M, Miyoshi N, Ohshima H.
  • Organizer: SFRRI 2014
  • Place of Presentation: Kyoto, Japan
• [Presentation] Denaturation of cellular proteins induced by secosterol aldehydes (2014)
  • Author(s): Miyoshi N, Tomono S, Ohshima H.
  • Organizer: SFRRI 2014
  • Place of Presentation: Kyoto, Japan
• [Presentation] LC/ESI-MS/MSによる肥満・糖尿病モデルマウス（ob/ob）血漿中アルデヒドの網羅的解析 (2013)
  • Author(s): 伴野勧、長澤美沙、村田晴美、真田峻介、三好規之、大島寛史
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] 神経細胞様分化PC-12へのamyloid-β曝露によって生成されるアルデヒド類の網羅的解析 (2013)
  • Author(s): 長澤美沙、伴野勧、三好規之、大島寛史
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] Cholesterol aldehydes: The mechanisms of their formation and pahological roles in oxidative stress related-disorders. (2013)
  • Author(s): Miyoshi N, Tomono S, Ohshima H.
  • Organizer: 3rd European Network for Oxysterol Research
  • Place of Presentation: Swansea, UK.
• [Presentation] Development of a comprehensive analytical method for lipophlic reactive carbonyl compounds in biological samples (2013)
  • Author(s): Tomono S, Miyoshi N, Ohshima H.
  • Organizer: SFRR-Europe 2013 Conference
  • Place of Presentation: Athens, Greece
• [Presentation] LC-ESI-MS/MSを用いた脂質過酸化物由来アルデヒド化合物の網羅的解析法の開発 (2012)
  • Author(s): 伴野勧、村田晴美、大畑美幸、三好規之、大島寛史
  • Organizer: 第85回日本生化学学会大会
  • Place of Presentation: 福岡
• [Presentation] Dehydrogenase/reductase member 2のアセトアルデヒド修飾はMdm2結合活性を低下させる (2012)
  • Author(s): 山田雄司、三好規之、伴野勧、大島寛史
  • Organizer: 第85回日本生化学学会大会
  • Place of Presentation: 福岡
• [Presentation] がん予防因子イソチオシアネート結合タンパク質の網羅的探索法 (2012)
  • Author(s): 三好規之、米持巧、伴野勧、中村宣督、大島寛史
  • Organizer: 第85回日本生化学学会大会
  • Place of Presentation: 福岡
• [Presentation] 疾病マーカー探索を指向したアルデヒド化合物の網羅的解析法の開発 (2012)
  • Author(s): 伴野勧、三好規之、大島寛史
  • Organizer: 第65回日本酸化ストレス学会学術集会
  • Place of Presentation: 徳島