| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Toxic aldehydes and ketones (that is, reactive carbonyl species; RCs) have been suspected of causing cardiovascular disease, neurodegenerative disease and other chronic diseases. In the present study, we have developed a highly sensitive and selective method that allowed us to detect numerous lipophilic RCs simultaneously. A new analytical method has been developed for profiling lipophilic RCs in biological samples using liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) with selected reaction monitoring (SRM). The method was found to be sensitive, reproducible, accurate and specific for RCs. Our novel approach could be very useful for establishing the RCs profiles of biological and environmental samples, as well as studying the roles of lipid peroxidation in oxidative stress related-disorders and discovering new biomarkers for the early diagnosis of these diseases.
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