Regulation of lipid metabolism by polyphenols derived from non-edible part

• Project/Area Number: 24700848
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Eating habits, studies on eating habits
• Research Institution: Tokyo University of Agriculture
• Principal Investigator: TAMURA Tomoko 東京農業大学, 応用生物科学部, 助教 (60451845)
• Co-Investigator(Renkei-kenkyūsha): MURA Kiyoshi 東京農業大学, 応用生物科学部, 教授 (40112883) ; ABE Naoki 東京農業大学, 応用生物科学部, 教授 (70291581) ; ARAI Soichi 東京農業大学, 応用生物科学部, 教授 (20011934)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: ポリフェノール / プロアントシアニジン / ラッカセイ種皮 / コレステロールミセル / 血糖値 / 糖質分解酵素阻害 / 重合度 / ピーナッツ種皮 / プロシアニジンA1 / 脂質代謝 / プロシアニジン / 構造決定 / 食品機能 / 腸管吸収

Research Abstract

We identified epicatechin-(4b->6)-epicatechin-(2b->O->7, 4b->8)-catechin (EEC, a proanthocyanidin trimer) in the skin of the peanut (Arachis hypogaea L.). EEC showed more potent cholesterol micelle-degrading activity than procyanidin A1 (a dimer) in vitro. The hypercholesterolemia suppressing effect of a peanut skin polyphenol on rats fed high-cholesterol diet in our preceding experiments might thus have been due primarily to a micelle-degrading effect in the intestine. Then we showed peanut skin extracts reduces blood glucose levels in rats administered starch, sucrose or glucose. Then inhibition activities of sugar digestion enzyme by proanthocyanidins from peanut skin were investigated and EEC inhibited alpha-amylase activity in a dose-dependent manner, while (+)-catechin and procyanidin A1 did not. These results indicate that procyanidins of different degrees of polymerization will different activities of cholesterol micelle-degrading activity and sugar digestion enzyme.

Research Products

• [Journal Article] Peanut-Skin Polyphenols, Procyanidin A1 and Epicatechin-(4 &#946;&#8594;6)-epicatechin-(2 &#946;&#8594;<i>O</i>&#8594;7, 4 &#946;&#8594;8)-catechin, Exert Cholesterol Micelle-Degrading Activity <i>in Vitro</i> (2013)
  • Author(s): Tomoko Tamura, Naoko Inoue, Megumi Ozawa, Akiko Shimizu-Ibuka, Soichi Arai, Naoki Abe, Hiroyuki Koshino, and Kiyoshi Mura
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 77 Issue: 6 Pages: 1306-1309
  • DOI: 10.1271/bbb.121023
  • NAID: 10031184700
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Peanut-SkinPolyphenols, Procyanidin A1 and Epicatechin-(4β→6)-epicatechin-(2β→O→7,4β→8)-catechin, Exert CholesterolMicelle-Degrading Activity in Vitro (2013)
  • Author(s): Tomoko Tamura, Naoko Inoue, Megumi Ozawa, Akiko Shimizu-Ibuka, Soichi Arai,Naoki Abe, Hiroyuki Koshino, and Kiyoshi Mura
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: -
  • NAID: 10031184700
  • Peer Reviewed
• [Presentation] ラッカセイ種皮プロアントシアニジンの抗酸化活性 (2013)
  • Author(s): 海老澤隆史、小澤恵実、田村倫子、村清司、荒井綜一
  • Organizer: 日本食品科学工学会
  • Place of Presentation: 実践女子学園
  • Year and Date: 2013-08-30
• [Book] 食べ物と健康―食品学 (2013)
  • Author(s): 大石裕一、服部一夫、田村倫子, 他
  • Publisher: 光生館
• [Remarks]
  • URL: http://www.nodai.ac.jp/safety/original/index.html