| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
In this project, I investigated to develop a new method using cultured cell to evaluate palytoxin content. As the results, a rat C6 glioma cell was suitable to use for a toxic evaluation for the palytoxin, furthermore I conclude that the method of detection by LDH release was sensitive. In addition, the method was applicable to the test solution that I prepared from toxic specimen Scarus ovifrons. On the other hand, peaks were detected in chromatogram of m/z 327.6, 1352.3 and 1360.3 in LC-MS analysis of the test solution. However did not come to conclude that it was the peak to originate from palytoxin or a palytoxin-like substance because the influence was inarticulate peak shape.
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