Assessment of biological effect of low-dose radiation by phosphorylated H2AX in vivo
| Project/Area Number |
24700955
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Carcinogenesis
|
|---|
| Research Institution | Ibaraki University (2013)
Osaka Medical College (2012) |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
|
|---|
| Keywords | 低線量放射線 / DNA損傷 / 発がん |
|---|
| Research Abstract |
Radiation induced-DNA double strand break (DSB) is one major initial cause of genomic instability leading to cancer. The creation of a DNA DSB in eukaryotic cells is generally accompanied by the formation of hundreds of phosphorylated H2AX (gamma-H2AX) molecules in the chromatin flanking the DSB site. Antibodies to gamma-H2AX allow the visualization of a "focus" at the DSB site. Since it has been demonstrated that the numbers of gamma-H2AX foci correlate directly to the numbers of DNA DSB, the detection of gamma-H2AX is an attractive candidate for monitoring DNA DSB levels. Here, to assess biological effect by radiation exposure after the accident, we performed quantitation of gamma-H2AX foci as a maker of DNA DSB in bovine lymphocytes from Fukushima evacuated area. Although majority of lymphocytes are negative of gamma-H2AX foci, immunostaining data clearly showed an increase of population of gamma-H2AX foci positive cells from Fukushima evacuated area.
|
Report
(3 results)
Research Products
(18 results)
-
[Journal Article] Mito-tempol and dexrazoxane exhibit cardioprotective and chemotherapeutic effects through specific protein oxidation and autophagy in a syngeneic breast tumor preclinical model.2013
Author(s)
Dickey JS, Gonzalez Y, Aryal B, Mog S, Nakamura AJ, Redon CE, Baxa U, Rosen E, Cheng G, Zielonka J, Parekh P, Mason KP, Joseph J, Kalyanaraman B, Bonner W, Herman E, Shacter E, Rao VA.
-
Journal Title
PLoS One.
Volume: 8
Issue: 8
Pages: e70575-e70575
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
