• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Assessment of biological effect of low-dose radiation by phosphorylated H2AX in vivo

Research Project

Project/Area Number 24700955
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Carcinogenesis
Research InstitutionIbaraki University (2013)
Osaka Medical College (2012)

Principal Investigator

NAKAMURA Asako  茨城大学, 理学部, 准教授 (70609601)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Keywords低線量放射線 / DNA損傷 / 発がん
Research Abstract

Radiation induced-DNA double strand break (DSB) is one major initial cause of genomic instability leading to cancer. The creation of a DNA DSB in eukaryotic cells is generally accompanied by the formation of hundreds of phosphorylated H2AX (gamma-H2AX) molecules in the chromatin flanking the DSB site. Antibodies to gamma-H2AX allow the visualization of a "focus" at the DSB site. Since it has been demonstrated that the numbers of gamma-H2AX foci correlate directly to the numbers of DNA DSB, the detection of gamma-H2AX is an attractive candidate for monitoring DNA DSB levels. Here, to assess biological effect by radiation exposure after the accident, we performed quantitation of gamma-H2AX foci as a maker of DNA DSB in bovine lymphocytes from Fukushima evacuated area. Although majority of lymphocytes are negative of gamma-H2AX foci, immunostaining data clearly showed an increase of population of gamma-H2AX foci positive cells from Fukushima evacuated area.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (18 results)

All 2014 2013 2012 Other

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (12 results) (of which Invited: 2 results) Book (4 results)

  • [Journal Article] Mito-tempol and dexrazoxane exhibit cardioprotective and chemotherapeutic effects through specific protein oxidation and autophagy in a syngeneic breast tumor preclinical model.2013

    • Author(s)
      Dickey JS, Gonzalez Y, Aryal B, Mog S, Nakamura AJ, Redon CE, Baxa U, Rosen E, Cheng G, Zielonka J, Parekh P, Mason KP, Joseph J, Kalyanaraman B, Bonner W, Herman E, Shacter E, Rao VA.
    • Journal Title

      PLoS One.

      Volume: 8 Issue: 8 Pages: e70575-e70575

    • DOI

      10.1371/journal.pone.0070575

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Use of the γ-H2AX assay to monitor DNA damage and repair in translational cancer research2012

    • Author(s)
      Ivashkevich A, Redon CE, Nakamura AJ, Martin RF, Martin OA.
    • Journal Title

      Cancer Lett.

      Volume: 327 Pages: 123133-123133

    • Related Report
      2012 Research-status Report
  • [Presentation] ヒストンH2AX による被ばくの検出―リン酸化ヒストンによる生体内DNA 損傷レベルの高感度検出2014

    • Author(s)
      中村麻子
    • Organizer
      第7回「Quantum Medicine 研究会」茨城大学重点研究「がん放射線治療のための基礎生命科学研究」講演会
    • Place of Presentation
      茨城大学(茨城)
    • Year and Date
      2014-03-09
    • Related Report
      2013 Final Research Report
  • [Presentation] 高感度DNA 損傷マーカーを用いた生体応答の評価2014

    • Author(s)
      中村麻子
    • Organizer
      平成26年東京 Radiation Biology Conference
    • Place of Presentation
      放射線医学総合研究所(千葉)
    • Year and Date
      2014-02-22
    • Related Report
      2013 Final Research Report
  • [Presentation] The use of γ-H2AX assay for validation of novel radioprotector against both acute and chronic low-dose radiation exposure2014

    • Author(s)
      Asako J. Nakamura, Megumi Sasatani and Kenji Kamiya
    • Organizer
      The 4th International Symposium of RIRBM, Hiroshima University
    • Place of Presentation
      広島大学(広島)
    • Related Report
      2013 Final Research Report
  • [Presentation] The use of γ-H2AX assay for validation of novel radioprotector against both acute and chronic low-dose radiation exposure2014

    • Author(s)
      Asako J. Nakamura, Megumi Sasatani and Kenji Kamiya
    • Organizer
      The 4th International Symposium of RIRBM, Hiroshima University
    • Place of Presentation
      広島
    • Related Report
      2013 Annual Research Report
  • [Presentation] Monitoring low-dose radiation induced-DNA damage levels in vivo2013

    • Author(s)
      中村麻子
    • Organizer
      日本放射線影響学会第56回大会
    • Place of Presentation
      パレス青森(青森)
    • Related Report
      2013 Final Research Report
  • [Presentation] Assessment of DNA damage level in animals from Fukushima area using phosphorylated H2AX assay2013

    • Author(s)
      中村麻子、鈴木正敏、福本学
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(神奈川)
    • Related Report
      2013 Final Research Report
  • [Presentation] Assessment of DNA damage level in animals from Fukushima area using phosphorylated H2AX assay2013

    • Author(s)
      Asako J Nakamura、Masatoshi Suzuki、Manabu Fukumoto
    • Organizer
      第72回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Related Report
      2013 Annual Research Report
  • [Presentation] Monitoring low-dose radiation induced-DNA damage levels in vivo2013

    • Author(s)
      Asako J Nakamura
    • Organizer
      日本放射線影響学会第56回大会
    • Place of Presentation
      青森
    • Related Report
      2013 Annual Research Report
  • [Presentation] Monitoring of low dose radiation induced-DNA damage level in vivo2012

    • Author(s)
      中 村麻子
    • Organizer
      日本放射線影響学会第55回大会
    • Place of Presentation
      東北大学(宮城)
    • Related Report
      2013 Final Research Report
  • [Presentation] Monitoring of low dose radiation induced-DNA damage level in vivo.2012

    • Author(s)
      中村麻子
    • Organizer
      第55回日本放射線影響学会
    • Place of Presentation
      仙台
    • Related Report
      2012 Research-status Report
  • [Presentation] 高感度DNA損傷マーカーを用いた生体応答の評価

    • Author(s)
      中村 麻子
    • Organizer
      平成26年東京Radiation Biology Conference
    • Place of Presentation
      千葉
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] ヒストンH2AXによる被ばくの検出―リン酸化ヒストンによる生体内DNA損傷レベルの高感度検出

    • Author(s)
      中村 麻子
    • Organizer
      第7回「Quantum Medicine研究会」茨城大学重点研究「がん放射線治療のための基礎生命科学研究」講演会
    • Place of Presentation
      茨城
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Book] Methods for the assessment of telomere status. Cell Senescence. Methods Mol. Biol. 9652013

    • Author(s)
      Nakamura AJ.
    • Related Report
      2013 Final Research Report
  • [Book] Methods Mol. Biol.2013

    • Author(s)
      Nakamura AJ
    • Total Pages
      538
    • Publisher
      Springer
    • Related Report
      2012 Research-status Report
  • [Book] γ-H2AX formation and chromatin structure. Advances in Genet. Res.2012

    • Author(s)
      Nakamura AJ, Parekh P, martin OA, Bonner WM and Redon CE
    • Related Report
      2013 Final Research Report
  • [Book] Advances in Genet. Res.2012

    • Author(s)
      Nakamura AJ, Parekh P, martin OA, Bonner WM and Redon CE.
    • Total Pages
      328
    • Publisher
      NOVA
    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi