Neuroblastoma mouse model developing metastatic tumors
| Project/Area Number |
24700957
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Carcinogenesis
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| Research Institution | Chiba Cancer Center (Research Institute) |
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Principal Investigator |
SUENAGA Yusuke 千葉県がんセンター(研究所), がんゲノムセンター, 研究員 (80581793)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | NCYM / MYCN / neuroblastoma / OCT4 / Myc-nick / stem cell / transgenic mouse / metastasis / N-CYM / p63 / Neuroblastoma / De novo gene birth / cis-antisense gene / De novo evolved protein / de novo gene birth / de novo evolved protein |
|---|
| Outline of Final Research Achievements |
MYCN oncogene has a central role in the genesis and progression of neuroblastomas, and its amplification is associated with an unfavorable prognosis. We have found that NCYM, a MYCN cis-antisense RNA, is evolutionally conserved only in the taxonomic group containing humans and chimpanzees and that the NCYM gene encodes a functional protein in humans. MYCN transgenic mice, which express human MYCN in sympathoadrenal tissues, spontaneously develop neuroblastomas. However, unlike human neuroblastoma, distant metastasis is infrequent. We established MYCN / NCYM double transgenic mice as a new animal model for studying neuroblastoma pathogenesis. We found that NCYM expression promoted both the metastasis and chemo-resistance of the neuroblastomas formed in the double transgenic mice. These results demonstrate that the MYCN / NCYM transgenic mouse may be a suitable model for the screening of the new drugs against high-risk neuroblastomas.
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Report
(4 results)
Research Products
(27 results)
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[Journal Article] NCYM, a Cis-antisense gene of MYCN, encodes a de novo evolved protein that inhibits GSK3β resulting in the stabilization of MYCN in human neuroblastomas.2014
Author(s)
Suenaga Y, Islam SM, Alagu J, Kaneko Y, Kato M, Tanaka Y, Kawana H, Hossain S, Matsumoto D, Yamamoto M, Shoji W, Itami M, Shibata T, Nakamura Y, Ohira M, Haraguchi S, Takatori A, Nakagawara A.
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Journal Title
PLoS Genet
Volume: 10
Issue: 1
Pages: 1-14
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] MYCNのアンチセンス遺伝子から進化したde novo geneであるNCYMはヒトの神経芽腫においてMYCN機能を制御する2014
Author(s)
末永 雄介, 庄子 渉, イスラム ラフィクル, アラグー ジェニファー, 金子 伊樹, 加藤 護, 田中 祐吉, 川名 秀忠, ホセイン シャミム, 伊丹 真紀子, 柴田 龍弘, 中村 洋子, 大平 美紀, 原口 清輝, 横井 左奈, 中川原 章
Organizer
第37回日本分子生物学会
Place of Presentation
横浜
Year and Date
2014-11-25 – 2014-11-27
Related Report
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[Presentation] NCYM, a de novo gene evolved from MYCN cis-antisense genes, encodes a protein regulating MYCN functions in human neuroblastomas2014
Author(s)
Yusuke Suenaga, Wataru Shoji, Rafiqul Islam, Jennifer Alagu, Yoshiki Kaneko, Mamoru Kato, Yukichi Tanaka, Hidetada Kawana, Shamim Hossain, Makiko Itami, Tatsuhiro Shibata, Yohko Nakamura, Miki Ohira, Seiki Haraguchi, Sana Yokoi, Akira Nakagawara
Organizer
The 9th 3R symposium
Place of Presentation
御殿場
Year and Date
2014-11-17 – 2014-11-21
Related Report
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[Presentation] MYCNのアンチセンス遺伝子から進化したde novo 遺伝子産物であるNCYMはヒトの神経芽腫においてMYCN機能を制御する2014
Author(s)
末永 雄介, 庄子 渉, ラフィクル イスラム, アラグー ジェニファー, 金子 伊樹, 加藤 護, 田中 祐吉, 川名 秀忠, シャミムホセイン, 伊丹 真紀子、 柴田 龍弘, 中村 洋子, 大平 美紀, 原口 清輝, 横井 左奈, 中川原 章
Organizer
第87回日本生化学会
Place of Presentation
京都
Year and Date
2014-10-15 – 2014-10-18
Related Report
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[Presentation] NCYM, a novel MYCN cis-antisense gene product, stabilizes MYCN and contributes to aggressiveness of human neuroblastoma2012
Author(s)
Yusuke Suenaga, Atsushi Takatori, Hidetada Kawana, Yoshiki Kaneko, Daisuke Matsumoto, Wataru Shoji, Mohammad Shamim Hossain, MikiOhira, Makiko Itami, Seiki Haraguchi, and Akira Nakagawara
Organizer
Advances in Neuroblastoma Research Conference 2012
Place of Presentation
トロント
Related Report
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[Presentation] NCYM, a cis-antisense gene of MYCN, is a TAp63 target gene that encode a de novo evolved protein stabilizing MYCN in human neuroblastoma
Author(s)
Yusuke Suenaga, S.M. Rafiqul Islam, Jennifer Alagu, Mamoru Kato, Yukichi Tanaka, Hidetada Kawana, Shamim Hossain, Daisuke Matsumoto, Mami Yamomoto, Wataru Shoji, Makiko Itami, Tatsuhiro Shibata, Yohko Nakamura, Miki Ohira, Seiki Haraguchi, Atsushi Takatori and Akira Nakagawara
Organizer
6th p63/p73 International Workshop
Place of Presentation
木更津
Related Report
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