| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
I developed murine in vitro reconstitution system of intestinal carcinogenesis. To clarify the potential roles of inflammation in intestinal carcinogenesis, I investigated the effects of transient co-culture with inflammatory cells. The intestinal cells infected shRNA against APC were only converted tumorigenic one after injected subcutaneously into nude mouse while those infected shRNA against vector control or p53 were not.
However, these shAPC transfected cells were converted more malignant one when co-cultured with inflammatory cells. Microarray analysis has been shown expression level of Chitinase-3-like protein 1 (CHI3L1). These results suggested that inflammation, co-cultured with inflammatory cells, allows tumor to subcutaneous proliferation ability.
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