Mechanism of inflammation-accelerate colorectal carcinogenesis by reconstitutive model
| Project/Area Number |
24700959
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Carcinogenesis
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| Research Institution | Tottori University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 炎症 / オルガノイド / 腸管発癌 / 発癌 / 癌遺伝子 / 癌抑制遺伝子 |
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| Research Abstract |
I developed murine in vitro reconstitution system of intestinal carcinogenesis. To clarify the potential roles of inflammation in intestinal carcinogenesis, I investigated the effects of transient co-culture with inflammatory cells. The intestinal cells infected shRNA against APC were only converted tumorigenic one after injected subcutaneously into nude mouse while those infected shRNA against vector control or p53 were not.
However, these shAPC transfected cells were converted more malignant one when co-cultured with inflammatory cells. Microarray analysis has been shown expression level of Chitinase-3-like protein 1 (CHI3L1). These results suggested that inflammation, co-cultured with inflammatory cells, allows tumor to subcutaneous proliferation ability.
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Report
(3 results)
Research Products
(10 results)
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[Journal Article] Fascin regulates chronic inflammation-related human colon carcinogenesis by inhibiting cell anoikis2014
Author(s)
Kanda Y, Kawaguchi T, Kuramitsu Y, Kitagawa T, Kobayashi T, Takahashi N, Tazawa H, Habelhah H, Hamada J-I, Kobayashi M, Hirahata M, Onuma K, Osaki M, Nakamura K, Kitagawa T, Hosokawa M and Okada F
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Journal Title
Proteomics
Volume: 14(9)
Pages: 1031-41
Related Report
Peer Reviewed
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[Journal Article] Fascin regulates chronic inflammation-related human colon carcinogenesis by inhibiting cell anoikis2014
Author(s)
Kanda Y, Kawaguchi T, Kuramitsu Y, Kitagawa T, Kobayashi T, Takahashi N, Tazawa H, Habelhah H, Hamada J-I, Kobayashi M, Hirahata M, Onuma K, Osaki M, Nakamura K, Kitagawa T, Hosokawa M and Okada F.
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Journal Title
Proteomics
Volume: 14
Issue: 9
Pages: 1031-1041
DOI
Related Report
Peer Reviewed
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