| Project/Area Number |
24700961
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | Ral / 低分子量GTP結合蛋白質 / Ras / 低分子量GTP結合タンパク質 |
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| Research Abstract |
The small GTPase RalA regulates many cellular processes such as cell proliferation, migration, membrane traffic, and tumorigenesis. Accumulating evidence shows that activation of RalA GTPase is essential for oncogenic Ras-induced human cell tumorigenesis. However, the molecular mechanism by which RalA regulates tumorigenesis remains elusive. I tried to identify RalA effector proteins in rat brain cytosol and succeeded in purifying a RalA effector complex using several chromatography steps including ammonium sulphate precipitation, ion-exchange chromatograhpy, and RalA affinity chromatography. This effector complex may be involved in RalA-dependent tumorigenesis.
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