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What is the melanoma-specific function of novel mutant MITF?

Research Project

Project/Area Number 24700971
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Tumor biology
Research InstitutionUniversity of Toyama

Principal Investigator

YOKOYAMA Satoru  富山大学, 和漢医薬学総合研究所, 助教 (90613498)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords悪性黒色腫 / がん遺伝子 / MITF
Outline of Final Research Achievements

Melanoma only diagnosed less than 10% of all form of skin cancer; however, it is responsible for the majority of skin cancer related death. In 2013, it was estimated that 76.690 new cases of melanoma will be diagnosed in Unites States, and 9.480 people will die due to this disease . When diagnosed in early stage and limited to the skin, the prognosis of melanoma is curable. When it becomes metastatic melanoma, the prognosis is very poor with a 5 years survival rate less than 20%.
By using the grant support, we determined the mechanism of drug-resistance in melanoma and of the suppression of melanoma growth by natural compounds.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (47 results)

All 2015 2014 2013 2012 Other

All Journal Article (19 results) (of which Peer Reviewed: 19 results,  Open Access: 1 results,  Acknowledgement Compliant: 4 results) Presentation (24 results) Book (1 results) Remarks (3 results)

  • [Journal Article] Critical contribution of MCL-1 in EMT-associated chemo-resistance in A549 non-small cell lung cancer2015

    • Author(s)
      Toge M, Yokoyama S, Kato S, Sakurai H, Senda K, Doki Y, Hayakawa Y, Yoshimura N, Saiki I
    • Journal Title

      Int. J. Oncol.

      Volume: 46 Issue: 4 Pages: 1844-1848

    • DOI

      10.3892/ijo.2015.2861

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Role of tyrosine kinase-independent phosphorylation of EGFR with activating mutation in cisplatin-treated lung cancer cells.2015

    • Author(s)
      Refaat A., Aminullah, Zhou Y., Kawanishi M., Tomaru R., Abdelhamed S., Shin M.S., Koizumi K., Yokoyama S., Saiki I., and Sakurai H.
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 458 Issue: 4 Pages: 856-861

    • DOI

      10.1016/j.bbrc.2015.02.044

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] RAC1 inhibition as a therapeutic target for gefitinib-resistant non-small cell lung cancer2014

    • Author(s)
      Kaneto N, Yokoyama S, Hayakawa Y, Kato S, Sakurai H, Saiki I
    • Journal Title

      Cancer Science

      Volume: 105 Issue: 7 Pages: 788-794

    • DOI

      10.1111/cas.12425

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Survivin suppression through STAT3/β-catenin is essential for resveratrol-induced melanoma apoptosis.2014

    • Author(s)
      Habibie, Yokoyama S, Abdelhamed S, Awale S, Sakurai H, Hayakawa Y, Saiki I.
    • Journal Title

      Int J Oncol.

      Volume: 45 Pages: 895-901

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Piperine Enhances the Efficacy of TRAIL-based Therapy for Triple-negative Breast Cancer Cells.2014

    • Author(s)
      Abdelhamed S, Yokoyama S, Refaat A, Ogura K, Yagita H, Awale S, Saiki I.
    • Journal Title

      Anticancer Res.

      Volume: 34 Pages: 1893-1899

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mesenchymal-transitioned cancer cells instigate the invasion of epithelial cancer cells through secretion of WNT3 and WNT5B2014

    • Author(s)
      Kato S, Hayakawa Y, Sakurai H, Saiki I, Yokoyama S
    • Journal Title

      Cancer Science

      Volume: 105 Issue: 3 Pages: 281-289

    • DOI

      10.1111/cas.12336

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] p38-Mediated phosphorylation of Eps15 endocytic adaptor protein.2014

    • Author(s)
      Zhou Y., Tanaka T., Sugiyama N., Yokoyama S., Kawasaki Y., Sakuma T., Ishihama Y., Saiki I., and Sakurai H.
    • Journal Title

      FEBS Letters

      Volume: 588 Issue: 1 Pages: 131-137

    • DOI

      10.1016/j.febslet.2013.11.020

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Procyanidin C1 from Cinnamomi Cortex inhibits TGF-β-induced epitheli al-to-mesenchymal transition in the A549 lung cancer cell line2013

    • Author(s)
      Kin R, Kato S, Kaneto N, Sakurai H, Hayakawa Y, Li F, Tanaka K, Saiki I, Yokoyama S
    • Journal Title

      International Journal of Oncology

      Volume: 43 Issue: 6 Pages: 1901-1906

    • DOI

      10.3892/ijo.2013.2139

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Berberine enhances tumor necrosis factor-related apoptosis-inducing ligand-mediated apoptosis in breast cancer.2013

    • Author(s)
      Refaat A, Abdelhamed S, Yagita H, Inoue H, Yokoyama S, Hayakawa Y, Saiki I.
    • Journal Title

      Oncol Lett.

      Volume: 6 Pages: 840-844

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] A flavonoid chrysin suppresses hypoxic survival and metastatic growth of mouse breast cancer cells.2013

    • Author(s)
      Lirdprapamongkol K, Sakurai H, Abdelhamed S, Yokoyama S, Maruyama T, Athikomkulchai S, Viriyaroj A, Awale S, Yagita H, Ruchirawat S, Svasti J, Saiki I.
    • Journal Title

      Oncol Rep.

      Volume: 30 Issue: 5 Pages: 2357-2364

    • DOI

      10.3892/or.2013.2667

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Inverse correlation between Thr-669 and constitutive tyrosine phosphorylation in the asymmetric epidermal growth factor receptor dimer conformation.2013

    • Author(s)
      Sato K., Shin M.S., Sakimura A., Zhou Y., Tanaka T., Kawanishi M., Kawasaki Y., Yokoyama S., Koizumi K., Saiki I., and Sakurai H.
    • Journal Title

      Cancer Science

      Volume: 104 Issue: 10 Pages: 1315-1322

    • DOI

      10.1111/cas.12225

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Myosin-Va contributes to manifestation of malignant-related properties in melanoma cells.2013

    • Author(s)
      Alves CP, Moraes MH, Sousa JF, Pontes CL, Ramão A, Yokoyama S, Trindade DM, Fisher DE, Espreafico EM.
    • Journal Title

      J Invest Dermatol.

      Volume: 33 Issue: 12 Pages: 2809-2812

    • DOI

      10.1038/jid.2013.218

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Chrysin overcomes TRAIL resistance of cancer cells through Mcl-1 downregulation by inhibiting STAT3 phosphorylation.2013

    • Author(s)
      Lirdprapamongkol K, Sakurai H, Abdelhamed S, Yokoyama S, Athikomkulchai S, Viriyaroj A, Awale S, Ruchirawat S, Svasti J, Saiki I.
    • Journal Title

      Int J Oncol.

      Volume: 43 Issue: 1 Pages: 329-337

    • DOI

      10.3892/ijo.2013.1926

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Oncogenic BRAF regulates oxidative metabolism via PGC1α and MITF.2013

    • Author(s)
      Haq R, Shoag J, Andreu-Perez P, Yokoyama S, Edelman H, et al.
    • Journal Title

      Cancer Cell

      Volume: 23 Issue: 3 Pages: 302-315

    • DOI

      10.1016/j.ccr.2013.02.003

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Haq R, Yokoyama S, Hawryluk EB, Jonsson GB, Frederick DT, et al.2013

    • Author(s)
      Haq R, Yokoyama S, Hawryluk EB, Jonsson GB, Frederick DT, et al.
    • Journal Title

      Proc Natl Acad Sci U S A

      Volume: 110 Issue: 11 Pages: 4321-4326

    • DOI

      10.1073/pnas.1205575110

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Identification of plant extracts sensitizing breast cancer cells to TRAIL.2013

    • Author(s)
      Abdelhamed S, Yokoyama S, Hafiyani L, Kalauni SK, Hayakawa Y, Awale S, Saiki I.
    • Journal Title

      Oncology Reports

      Volume: 29 Issue: 5 Pages: 1991-1998

    • DOI

      10.3892/or.2013.2293

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Gomisin A enhances tumor necrosis factor-α-induced G1 cell cycle arrest via signal transducer and activator of transcription 1-mediated phosphorylation of retinoblastoma protein.2012

    • Author(s)
      Waiwut P, Shin MS, Yokoyama S, Saiki I, Sakurai H.
    • Journal Title

      Biol Pharm Bull

      Volume: 35 Pages: 1997-2003

    • NAID

      130001872260

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Uvaridacols E-H, Highly Oxygenated Antiausterity Agents from Uvaria dac.2012

    • Author(s)
      Awale S, Ueda JY, Athikomkulchai S, Dibwe DF, Abdelhamed S, Yokoyama S, Saiki I, Miyatake R.
    • Journal Title

      J Nat Prod

      Volume: 75 Pages: 1999-2002

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Anti-austerity agents from Uvaria dac and their preferential cytotoxic activity against human pancreatic cancer cell lines in nutrient deprived condition.2012

    • Author(s)
      Awale S,Ueda J,Athikomkulchai S,Abdelhamed S,Yokoyama S,Saiki I,Miyatake R.
    • Journal Title

      J Nat Prod

      Volume: 75 Issue: 6 Pages: 1177-1183

    • DOI

      10.1021/np300295h

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Effect of Keishibukuryogan on genetic and diet-induced obesity models.2014

    • Author(s)
      Gao F., Yokoyama S., Fujimoto M., Shimada Y., Saiki I. and Hayakawa Y.
    • Organizer
      17th International Conference of Oriental Medicine
    • Place of Presentation
      Taiwan
    • Year and Date
      2014-11-01 – 2014-11-03
    • Related Report
      2014 Annual Research Report
  • [Presentation] Secretory CTGF mediates autonomous MET-like alterations in mesenchymal-transitioned A549 cells.2014

    • Author(s)
      Kato S., Yokoyama S., Hayakawa Y. and Saiki I.
    • Organizer
      The 73rd Annual Meeting of the Japanese Cancer Association.
    • Place of Presentation
      横浜
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Piperine enhances the efficacy of trail-based therapy for triple-negative breast cancer cells.2014

    • Author(s)
      Abdelhamed S., Yokoyama S., Refaat A., Ogura K., Awale S. and Saiki I.
    • Organizer
      The 73rd Annual Meeting of the Japanese Cancer Association.
    • Place of Presentation
      横浜
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] メラノーマの転移抑制を目指したSLUGに対する脱ユビキチン化酵素の探索2014

    • Author(s)
      岩上雄亮、横山 悟、兼頭直希、加藤真一郎、早川芳弘、済木育夫
    • Organizer
      The 73rd Annual Meeting of the Japanese Cancer Association.
    • Place of Presentation
      横浜
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 腎がん・肺がんの転移抑制活性を有する生薬の探索とその機序解析2014

    • Author(s)
      横山 悟、金 諒英、村田 純、岩下 淳、加藤真一郎、兼頭直希、櫻井宏明、李 峰、田中 謙、早川芳弘、済木育夫
    • Organizer
      日本生薬学会第61回年会
    • Place of Presentation
      福岡
    • Year and Date
      2014-09-13 – 2014-09-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] 漢方シグナルパネルの構築とその応用2014

    • Author(s)
      江島 静、横山 悟、早川芳弘、阿部貴志、済木育夫
    • Organizer
      第31回和漢医薬学会総会
    • Place of Presentation
      千葉
    • Year and Date
      2014-08-30 – 2014-08-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] Sensitization for anti-cancer drug by Saikosaponin B22014

    • Author(s)
      Ma H., Yokoyama S., Saiki I. and Hayakawa Y.
    • Organizer
      第31回和漢医薬学会総会
    • Place of Presentation
      千葉
    • Year and Date
      2014-08-30 – 2014-08-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] Identification of novel anti-inflammatory compounds from Morus alba Linne by targeting NF-B2014

    • Author(s)
      Hardianti B., Li F., Yokoyama S., Saiki I. and Hayakawa Y.
    • Organizer
      第31回和漢医薬学会総会
    • Place of Presentation
      千葉
    • Year and Date
      2014-08-30 – 2014-08-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] 細胞内シグナルからみる天然薬物2014

    • Author(s)
      横山 悟、江島 静、阿部貴志、済木育夫
    • Organizer
      天然薬物研究方法論アカデミー 第17回富山シンポジウム
    • Place of Presentation
      富山
    • Year and Date
      2014-07-26 – 2014-07-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 肺がんにおけるSNAILに対する脱ユビキチン化酵素の探索2014

    • Author(s)
      横山 悟、兼頭直希、加藤真一郎、早川芳弘、済木育夫
    • Organizer
      第23回日本がん転移学会
    • Place of Presentation
      金沢
    • Year and Date
      2014-07-10 – 2014-07-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] survivinの抑制がレスベラトロールによる悪性黒色腫のアポトーシスに必須である2014

    • Author(s)
      横山 悟、Habibie、Abdelhamed Sherif、Awale Suresh、櫻井 宏明、早川 弘、済木育夫
    • Organizer
      文部省新学術領域研究・がん支援「化学療法基盤支援活動」 第3回シンポジウム:アカデミアからの抗がん剤創薬に向けて 天然物の有効利用
    • Place of Presentation
      沖縄
    • Year and Date
      2014-05-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] Resveratrol induces apoptosis in melanoma cells via downregulation of survivin expression.2013

    • Author(s)
      Saiki I., Habibie, Abdelhamed S., Awale S., Hayakawa Y. and Yokoyama S.
    • Organizer
      8th World Congress on Advances in Oncology and 16th International Symposium on Molecular Medicine
    • Place of Presentation
      Greece
    • Related Report
      2013 Research-status Report
  • [Presentation] Identification of plant extracts sensitizing breast cancer cells to TRAIL.2013

    • Author(s)
      Abdelhamed S., Yokoyama S., Lia Hafiyani, Kalauni S.K., Hayakawa Y., Awale S. and Saiki I.
    • Organizer
      第72回日本癌学会総会
    • Place of Presentation
      横浜
    • Related Report
      2013 Research-status Report
  • [Presentation] Mesenchymal-transitioned cancer cells instigate surrounding cancer cells to metastasis via secretion of WNT ligands.2013

    • Author(s)
      Kato S., Yokoyama S., Hayakawa Y. and Saiki I.
    • Organizer
      第72回日本癌学会総会
    • Place of Presentation
      横浜
    • Related Report
      2013 Research-status Report
  • [Presentation] RAC1 inhibitor suppresses gefitinib-resistant NSCLC cell migration.2013

    • Author(s)
      Kaneto N., Kato S., Yokoyama S. and Saiki I.
    • Organizer
      第72回日本癌学会総会
    • Place of Presentation
      横浜
    • Related Report
      2013 Research-status Report
  • [Presentation] Resveratrol induces apoptosis in melanoma cells via downregulation of survivin expression.2013

    • Author(s)
      Habibie, Abdelhamed S., Awale S., Hayakawa Y., Saiki I. and Yokoyama S.
    • Organizer
      第72回日本癌学会総会
    • Place of Presentation
      横浜
    • Related Report
      2013 Research-status Report
  • [Presentation] 桂皮由来のProcyanidin C1によるTGF-依存性上皮間葉転換を抑制する機能解析2013

    • Author(s)
      金 諒英、加藤真一郎、兼頭直希、櫻井宏明、早川芳弘、李 峰、田中 謙、済木育夫、横山 悟
    • Organizer
      第30回和漢医薬学会総会
    • Place of Presentation
      金沢
    • Related Report
      2013 Research-status Report
  • [Presentation] Resveratrol induces apoptosis in melanoma cells via downregulation of surviving expression.2013

    • Author(s)
      , Yokoyama S., Abdelhamed S., Awale S., Hayakawa Y. and Saiki I.
    • Organizer
      Second Makassar International Symposium on Pharmaceutical Science.
    • Place of Presentation
      Indonesia
    • Related Report
      2013 Research-status Report
  • [Presentation] 不均一ながん細胞間の相互作用がEMT/METを制御する2012

    • Author(s)
      加藤真一郎,横山悟,済木育夫
    • Organizer
      第21回日本がん転移学会学術集会
    • Place of Presentation
      広島
    • Related Report
      2012 Research-status Report
  • [Presentation] ゲフィチニブによる肺がん転移抑制機構の解析2012

    • Author(s)
      兼頭直希,加藤真一郎,横山悟,済木育夫
    • Organizer
      第21回日本がん転移学会学術集会
    • Place of Presentation
      広島
    • Related Report
      2012 Research-status Report
  • [Presentation] 上皮間葉転換を抑制する桂皮の機能解析2012

    • Author(s)
      金諒英,加藤真一郎,兼頭直希,櫻井宏明,早川芳弘,李峰,田中謙,横山悟,済木育夫
    • Organizer
      第29回和漢医薬学会総会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] Mesenchymal-transitioned Epithelial cancer cells instigate EMT via pancreatic WNT signals2012

    • Author(s)
      Kato S., Yokoyama S., Hayakawa Y., and Saiki I.
    • Organizer
      14th International Biennial Congress of the Metastasis Research Society
    • Place of Presentation
      Brisbane, Australia
    • Related Report
      2012 Research-status Report
  • [Presentation] Berberine-induced sensitization of metastatic breast cancer to TRAIL treatment2012

    • Author(s)
      Refaat A., Diaa S., Yagita H., Yokoyama S., Hayakawa Y. and Saiki I.
    • Organizer
      第71回日本癌学会総会
    • Place of Presentation
      札幌
    • Related Report
      2012 Research-status Report
  • [Presentation] 間葉転換した上皮系がん細胞に付与されるパラクラインWNTシグナルはEMTの更なる助長を導く2012

    • Author(s)
      加藤真一郎,横山悟,早川芳弘,済木育夫
    • Organizer
      第71回日本癌学会総会
    • Place of Presentation
      札幌
    • Related Report
      2012 Research-status Report
  • [Book] 生化学 第85巻 第6号2013

    • Author(s)
      横山 悟
    • Total Pages
      6
    • Publisher
      日本生化学会
    • Related Report
      2013 Research-status Report
  • [Remarks] 和漢研 病態生化学 研究業績

    • URL

      http://www.inm.u-toyama.ac.jp/pb/gyoseki/index.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] 和漢研 病態生化学 研究業績

    • URL

      http://www.inm.u-toyama.ac.jp/pb/gyoseki/index.html

    • Related Report
      2013 Research-status Report
  • [Remarks] 和漢研・病態生化学 研究業績

    • URL

      http://www.inm.u-toyama.ac.jp/pb/gyoseki/index.html

    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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