| Project/Area Number |
24700974
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
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| Research Institution | Japan Advanced Institute of Science and Technology |
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Principal Investigator |
SUZUKI Hitoshi 北陸先端科学技術大学院大学, マテリアルサイエンス研究科, 特任助教 (00447690)
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| Research Collaborator |
TSUKAHARA Toshifumi
MAYEDA Akila
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 選択的スプライシング / ヌクレオフォスミン / 細胞周期 |
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| Outline of Final Research Achievements |
NPM (Nucleophosmin) is a multifunctional protein, such as a regulator of the centrosome duplication. It was reported that the phospho-Thr(199) NPM departed the centrosome and localized into the nuclear speckle. Also, this protein had a repressive activity of the pre-mRNA splicing in vitro. However, the regulation of alternative splicing by NPM was unclear. Using total RNAs of the cells over-expressing T199D-NPM (that imitates functionalities of phospho-Thr(199) NPM), we carried out the Exon Array analysis to identify splicing targets of NPM. Unfortunately, the validation by the RT-PCR suggested that most extracted exons were not actual target exons of NPM. Nevertheless, several alternative exons including the splicing events of a cell cycle-related G-protein and a leukemia-related transcription factor were obtained as potential targets of NPM. These may play critical roles in the alternative splicing by NPM.
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