Identification of key factors of glioblastoma tumorigenesis

• Project/Area Number: 24700986
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor biology
• Research Institution: Keio University
• Principal Investigator: SAMPETREAN Oltea 慶應義塾大学, 医学部, 特任助教 (50571113)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 癌 / 遺伝子 / 細胞・組織 / 幹細胞 / グリオブラストーマ / 悪性脳腫瘍

Research Abstract

The goal of this study was to investigate the characteristics of glioma-initiating cells (GICs) and identify key factors regulating the formation of glioblastoma. Using our syngeneic murine model, we conducted research as follows.
(1) We established several GIC clones and analyzed their basic properties, such as proliferation,invasion and ability to induce microvascular proliferation. As a result, we found that metabolic characteristics such as glucose consumption and lactic acid production can differ significantly between clones. (2) We analyzed the gene expression profile of our GIC clones and identified factors involved in the regulation of their metabolic characteristics. (3) We performed a functional analysis of the data obtained in (1), (2) and showed that GICs can use not only glycolysis, but also oxidative phosphorylation during glioblastoma formation.

Research Products

• [Journal Article] Integrated analysis identifies different metabolic signatures for tumor-initiating cells in a murine glioblastoma model (2014)
  • Author(s): Saga I, Shibao S, Okubo J, Osuka S, Kobayashi Y, Yamada S, Fujita S, Urakami K, Kusuhara M, Yoshida K, Saya H, Sampetrean O
  • Journal Title: Neuro-Oncology Volume: (in press)
  • Peer Reviewed
• [Journal Article] Characteristics of glioma stem cells (2013)
  • Author(s): Sampetrean O and Saya H
  • Journal Title: Brain Tumor Pathol Volume: 30(4) Issue: 4 Pages: 209-14
  • DOI: 10.1007/s10014-013-0141-5
  • Peer Reviewed
• [Journal Article] Characteristics of glioma stem cells (2013)
  • Author(s): Sampetrean O
  • Journal Title: Brain Tumor Pathology Volume: In press
  • Peer Reviewed
• [Presentation] Integrated analysis identifies different metabolic signatures for tumor-initiating cells in a murine glioblastoma model (2014)
  • Author(s): Sampetrean O
  • Organizer: Keystone Symposia–x5 Tumor Metabolism
  • Place of Presentation: Whistler, Canada
  • Year and Date: 2014-03-19
• [Presentation] Anti-invasion strategies in an induced cancer stem cell model of glioblastoma (2012)
  • Author(s): Sampetrean O
  • Organizer: 19th International Brain Tumor Research and Therapy Conference
  • Place of Presentation: Niagara Falls, Canada
  • Year and Date: 2012-06-23
• [Presentation] Sampetrean O : Invasion characteristics of tumor cells in a glioblastoma model of genetically modified neural stem cells (2012)
  • Author(s): Sampetrean O
  • Organizer: 103 Annual Meeting of American Association for Cancer Research
  • Place of Presentation: Chicago, USA
  • Year and Date: 2012-03-03
• [Presentation] Invasion characteristics of tumor cells in a glioblastoma model of genetically modified neural stem cells (2012)
  • Author(s): Sampetrean O
  • Organizer: 103 Annual Meeting of American Association for Cancer Research
  • Place of Presentation: Chicago, USA
• [Presentation] Integrated analysis identifies different metabolic signatures for tumor-initiating cells in a murine glioblastoma model
  • Author(s): Oltea Sampetrean
  • Organizer: Keystone Symposia - Tumor Metabolism
  • Place of Presentation: Whistler, Canada
• [Remarks]
  • URL: http://genereg.jp/index.html
• [Remarks] 慶應義塾大学 医学部 先端医科学研究所 遺伝子制御研究部門 ホームページ
  • URL: http://www.genereg.jp/