| Project/Area Number |
24700986
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
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| Research Institution | Keio University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 癌 / 遺伝子 / 細胞・組織 / 幹細胞 / グリオブラストーマ / 悪性脳腫瘍 |
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| Research Abstract |
The goal of this study was to investigate the characteristics of glioma-initiating cells (GICs) and identify key factors regulating the formation of glioblastoma. Using our syngeneic murine model, we conducted research as follows.
(1) We established several GIC clones and analyzed their basic properties, such as proliferation,invasion and ability to induce microvascular proliferation. As a result, we found that metabolic characteristics such as glucose consumption and lactic acid production can differ significantly between clones. (2) We analyzed the gene expression profile of our GIC clones and identified factors involved in the regulation of their metabolic characteristics. (3) We performed a functional analysis of the data obtained in (1), (2) and showed that GICs can use not only glycolysis, but also oxidative phosphorylation during glioblastoma formation.
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