| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
C1D, a DNA binding protein, is involved in basal transcription and DNA repair. However, the function of C1D remains elusive. In the present study, we examined the dynamics of C1D protein in the nucleosomes and the role of C1D in IL-8 transcription induced by ultraviolet (UV) irradiation. C1D protein normally binds the nucleosomes in HeLa cells. UV induced a rapid decrease of nucleosomal C1D by an E3 ligase CHIP (carboxyl terminus of the heat shock cognate protein 70 interacting protein)-mediated proteasome-dependent degradation and subsequently up-regulation of IL-8 transcription. Our studies revealed that C1D plays roles in a chromatin-associated transcriptional suppressor, and the homeostatic maintenance of C1D protein regulates IL-8 transcription.
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