| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
This project was designed to ascertain the dedifferentiation of adipocytes in cancer tissue and stabilization of tumor angiogenesis by pericytes derived from adipocytes. Mature adipocytes co-cultured with TGF-beta treated NMuMG cells (EMT cells) were enhanced dedifferentiation. Mouse dedifferentiated fat cells (DFAT) were migrated to endothelial tube formations in 3D gel assay, and expressed pericyte markers, NG2 and ASMA . When cancer cells and DFAT were transplanted into mouse subcutaneous tissue, DFAT were observed on the surface of tumor vessels expressing NG2 and reduced vessel density in cancer tissue. These findings suggest that some signals from cancer cells stimulate dedifferentiation of mature adipocytes and these dedifferentiated adipocytes enhanced stabilization of tumor angiogenesis by becoming the source of pericytes.
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