Studies on the molecular biological implications of dendritic cell surface Hsp90
| Project/Area Number |
24700993
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor immunology
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| Research Institution | Okayama University |
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Principal Investigator |
YAMAZAKI Chihiro 岡山大学, 医歯(薬)学総合研究科, 助教 (60620995)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 免疫学 / Hsp90 / 樹状細胞 / エンドサイトーシス / クロスプレゼンテーション |
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| Research Abstract |
Several monoclonal antibodies to Hsp90 have been established, and one of them, clone 5H12, revealed that Hsp90 existed on dendritic cell surface. Because Hsp90 does not have a transmembrane domain, it should need to make a complex with a transmembrane protein. To analyze this cell surface complex, 6H8 was used to immunoprecipitate Hsp90 on the dendritic cell line, DC2.4, and the complex contained at least Hsp90, Hsc70, Hsp70-Hsp90 organizing protein (Hop).
We also have data which shows that antigen conjugated to another mAb to Hsp90, 6H8, efficiently cross-presented in vivo. To clarify the mechanisms of this phenomenon, we assayed internalization of 5H12 to DC2.4 and found that 85% of surface-bound 5H12 was internalized to DC2.4 within 15 minutes. This rapid and efficient internalization should be an explanation to immunostimulating effect of mAb to Hsp90.
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Report
(3 results)
Research Products
(9 results)
