Comprehensive metabolome analysis with high resolution and sensitve system

• Project/Area Number: 24701005
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor diagnosis
• Research Institution: Kobe University
• Principal Investigator: IRINO Yasuhiro 神戸大学, 医学(系)研究科(研究院), 特命助教 (10415565)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 代謝 / メタボローム解析 / メタボロミクス / がん / バイオマーカー

Research Abstract

Metabolomics is a powerful approach to discover new biomarkers of various diseases. In this study, we tried to develop the high sensitive and resolution metabolome analysis system.
We first focused on a tumor suppressor gene, Adenomatous polyposis coli (APC). We conducted the comprehensive quantification of water-soluble metabolites and proteins in colon cancer cells (both APC mutation cell and APC wild-type cell) using a mass spectrometry. We determined target metabolites for our system. We also found the levels of some metabolic enzymes were regulated by APC activity. These results suggest that APC mutation causes metabolite pathways in colon cancers.

Research Products

• [Journal Article] Phosphatidylinositol 4-phosphate in the Golgi apparatus regulates cell-cell adhesion and invasive cell migration in human breast cancer. (2014)
  • Author(s): Tokuda, E., Itoh, T., Hasegawa, J., Ijuin, T., Takeuchi, Y., Irino, Y., Fukumoto, M., Takenawa, T.
  • Journal Title: Cancer Res. Volume: 74 Issue: 11 Pages: 3054-3066
  • DOI: 10.1158/0008-5472.can-13-2441
  • Peer Reviewed / Open Access
• [Journal Article] Diagnosis of gastroenterological diseases by metabolome analysis using gas chromatography-mass spectrometry (2012)
  • Author(s): Yoshida M, Hatano N, Nishiumi S, Irino Y, Izumi Y, Takenawa T, Azuma T
  • Journal Title: J. Gastroenterol Volume: 47(1) Issue: 1 Pages: 9-20
  • DOI: 10.1007/s00535-011-0493-8
  • NAID: 10030295488
  • Peer Reviewed
• [Presentation] 入野康宏,平田悠、竹内由紀子、波多野直哉、山田小弓、竹縄忠臣PI3阻害剤が代謝に与える影響のプロファイリング (2013)
  • Author(s): 入野康宏,平田悠、竹内由紀子、波多野直哉、山田小弓、竹縄忠臣
  • Organizer: 第65回日本細胞生物学会
  • Place of Presentation: 名古屋 (フラッシュトーク発表、ポスター発表)
  • Year and Date: 2013-06-21
• [Presentation] The Profiling of the Effects of Adenomatous Polyposis Coli (APC) Mutation on the Cellular Proteome in Colorectal Cancer Cells. (2013)
  • Author(s): Irino Y, Koshiyama A, Ichibangase T, Imai K, Yoshida M.
  • Organizer: HUPO 12th Annual World Congress
  • Place of Presentation: Yokohama
• [Presentation] PI3阻害剤が代謝に与える影響のプロファイリング (2013)
  • Author(s): 入野 康宏, 平田 悠、竹内 由紀子、波多野 直哉、山田 小弓、竹縄 忠臣
  • Organizer: 第65回日本細胞生物学会
  • Place of Presentation: 名古屋
• [Presentation] Characterization of breast cancer cells with metabolic profiling (2012)
  • Author(s): 入野康宏,平田悠、竹内由紀子、波多野直哉、竹縄忠臣
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡 (ポスター発表)
  • Year and Date: 2012-12-15
• [Presentation] The Profiling of the Effects of Adenomatous Polyposis Coli (APC) Mutation on the Cellular Proteome in Colorectal Cancer Cells (2012)
  • Author(s): Irino Y, Koshiyama A, Ichibangase T, Imai K, Yoshida M
  • Organizer: HUPO 12^<th> Annual World Congress
  • Place of Presentation: Pacifico Yokohama, Japan (Poster presentation)
  • Year and Date: 2012-09-17