Basic research for early diagnosis of lung carcinomas by the application of DNA methylation analysis
| Project/Area Number |
24701008
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor diagnosis
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| Research Institution | Kagoshima University |
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Principal Investigator |
YOKOYAMA Seiya 鹿児島大学, 医歯(薬)学総合研究科, 助教 (20569941)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | lung adenocarcinomas / mucin / DNA methylation / demethylation / ten eleven translocation / 肺癌 / ムチン / エピジェネティクス / DNA脱メチル化 |
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| Research Abstract |
Lung cancer is still a disease of high mortality, despite advanced diagnostic techniques. Mucins (MUC) play crucial roles in carcinogenesis and tumor invasion in lung neoplasms. We have developed a novel 'methylation-specific electrophoresis (MSE)' method to analyze the DNA methylation status of MUC1 and MUC4 by high sensitivity and resolution.
By using the MSE method, we evaluated lung tissue samples from 33 patients with various lung lesions. Our results showed that the TET1 was important factor for DNA demethylation to regulate the expression of MUC1 and MUC4 in lung cancer. The analysis of the epigenetic changes of MUC1 and MUC4 may be useful for diagnosing the carcinogenic risk and predicting outcome of patients.
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Report
(3 results)
Research Products
(41 results)
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[Journal Article] Diagnosis of pancreatic neoplasms using a novel method of DNA methylation analysis of mucin expression in pancreatic juice2014
Author(s)
Yokoyama S, Kitamoto S, Higashi M, Goto Y, Hara T, Ikebe D, Yamaguchi T, Arisaka Y, Niihara, Nishimata H, Tanaka S, Takaori K, Batra S K, Yonezawa S
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Journal Title
PLoS ONE
Volume: 9(4)
Issue: 4
Pages: e93760-e93760
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Pathobiological implications of mucin (MUC) expression in the outcome of small bowel cancer2014
Author(s)
Shibahara H, Higashi M, Koriyama C, Yokoyama S, Kitazono I, Yasuhiro K, Narita M, Kuze S, Takanori K, Mita S, Arai T, Kato T, Yuasa N, Yamaguchi R, Kubota H, Suzuki H, Baba S, Rousseau K, Batra S K, Yonezawa S
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Journal Title
PLoS ONE
Volume: 9(4)
Issue: 4
Pages: e86111-e86111
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Expression of MUC4 mucin is observed mainly in the intestinal-type of intraductal papillary mucinous neoplasm of the pancreas2013
Author(s)
Kitazono I, Higashi M, Kitamoto S, Yokoyama S, Horinouchi M, Osako M, Shimizu T, Tabata M, Batra S K, Goto M, Yonezawa S
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Journal Title
Pancreas
Volume: 42(7)
Issue: 7
Pages: 1120-1128
DOI
Related Report
Peer Reviewed
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[Journal Article] MUC4 and MUC1 Expression in Adenocarcinoma of the Stomach Correlates with Vessel Invasion and Lymph Node Metastasis : An Immunohisto chemical Study of Early Gastric Cancer.2013
Author(s)
Yukihiro Tamura, Michiyo Higashi, Sho Kitamoto, Seiya Yokoyama, Masahiko Osako, Michiko Horinouchi, Takeshi Shimizu, Mineo Tabata, Surinder K. Batra, Masamichi Goto, Suguru Yonezawa
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Journal Title
PLoS ONE
Volume: (in press)
Issue: 11
Pages: e49251-e49251
DOI
Related Report
Peer Reviewed
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[Presentation] The Application of New DNA Methylation Analysis, Designated Methylation Specific Electrophoresis (MSE) for Pancreatic Juice Analysis2012
Author(s)
Yokoyama S, kitamoto S, Hara T, Arisaka Y, Takaori K, Yamamoto T, Niihara T, Nishimata H, Tanaka S, Higashi M, Yonezawa S
Place of Presentation
京都国際コンベンションセンター(京都)
Related Report
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