| Project/Area Number |
24701012
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor diagnosis
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
FUJIOKA Kouki 東京慈恵会医科大学, 医学部, 講師 (90392381)
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| Research Collaborator |
TAKEYAMA Hiroshi 東京慈恵会医科大学, 医学部, 教授 (70236511)
MANOME Yoshinobu 東京慈恵会医科大学, 医学部, 教授 (30219539)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 甲状腺癌 / 癌抗原 / 診断 / 抗体 / 血液検査法 / スクリーニング / フィブロネクチン / 質量分析 / 糖鎖 |
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| Outline of Final Research Achievements |
We investigated the new detection method of thyroid carcinoma antigen by combining fluorescence with the JT 95 antibody for the specific recognition of thyroid cancer. In this study, we discovered several new candidate proteins with immunoprecipitation method. Moreover, in a pilot study, the antigens in the smear cellular sample from papillary carcinoma tissue was detected clearly with biotinylated JT95 antibody and phycoerythrin. However, antigen detection system in blood plasma samples with JT95 sandwich ELISA did not show the specificity against thyroid carcinoma. Therefore, the accurate antigen identification will be needed for developing the new detection system .
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