| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
We report on high-throughput screening system using autoantibody phage library for finding novel diagnostic biomarker of gastric cancer. The antibody phage library was constructed from peripheral blood lymphocytes from gastric cancer patients using polymerase chain reaction. We panned the constructed library with scirrhous gastric cancer and disease-free control serum to make a sublibrary of antibodies that bind proteins differentially expressed, structurally changed, and post-translationally modified. To confirm this, we examined the binding ability of enriched antibody phage using ELISA. The results indicated that they were binding against gastric cancer serum. In the future, we will perform to isolate antibody phage clone specific to gastric cancer serum.
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