Quantification of the contribution of each pathway in N2O production from nitrifying bacteria by using a dual-labeled tracer technique
Project/Area Number |
24710012
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Environmental dynamic analysis
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Research Institution | Chuo University |
Principal Investigator |
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Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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Keywords | 安定同位体トレーサー / アンモニア酸化細菌 / 亜酸化窒素 / 硝化 / 脱窒 / 窒素循環 / 温室効果ガス / ガスクロマトグラフィー質量分析計 / 好気性アンモニア酸化細菌 / 国際情報交換(USA、カナダ) / 硝化菌脱窒 |
Outline of Final Research Achievements |
Ammonia-oxidizing bacteria (AOB) have been shown to produce nitrous oxide (N2O) from hydroxylamine (NH2OH) during ammonia oxidization and/or from nitrite (NO2-) during nitrifier denitrification under aerobic condition. The aim of this study is to determine which mechanisms are responsible for producing N2O by AOB species using a 15N and 18O tracer technique. (1) The fragmentation and the scrambling ratio of N2O were determined by GC/MS. (2) Cell suspension of Nitrosomonas strains formed hybrid N2O from NH2OH and NO2-. The dominant pathway in N2O production differed among Nitrosomonas strains under a condition, where they were allowed to grow optimally.
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Report
(4 results)
Research Products
(4 results)