Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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Outline of Final Research Achievements |
This study developed new method to identify the pollen grains in ice cores at the species level by whole genome amplification method that may provide sufficient sequence data for the identification. Pollen grains used in this study were Pinus extracted from a ice core drilled from the Belukha glacier in the Altai Mountains of Russia in the summer of 2003. The developed new method was performed using a single pollen grain. The pollen grains from Belukha glacier were identified as P. Sylvestris, which are currently found at the periphery of the glacier. In addition, the DNA analyses indicated that P. Sylvestris is a dominant species in genus Pinus in this region.
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