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Paleoenvironment reconstruction using the genetic information of a single pollen grain from ice cores

Research Project

Project/Area Number 24710019
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Environmental dynamic analysis
Research Institution大学共同利用機関法人情報・システム研究機構(新領域融合研究センター及びライフサイ

Principal Investigator

NAKAZAWA Fumio  大学共同利用機関法人情報・システム研究機構(新領域融合研究センター及びライフサイ, 大学共同利用機関等の部局等, 研究員 (80432178)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords氷河 / 花粉 / DNA / アイスコア / マツ属 / WGA / DNA / ロシア・アルタイ山脈 / ロシア / アルタイ山脈
Outline of Final Research Achievements

This study developed new method to identify the pollen grains in ice cores at the species level by whole genome amplification method that may provide sufficient sequence data for the identification. Pollen grains used in this study were Pinus extracted from a ice core drilled from the Belukha glacier in the Altai Mountains of Russia in the summer of 2003. The developed new method was performed using a single pollen grain. The pollen grains from Belukha glacier were identified as P. Sylvestris, which are currently found at the periphery of the glacier. In addition, the DNA analyses indicated that P. Sylvestris is a dominant species in genus Pinus in this region.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (20 results)

All 2014 2013 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (11 results) (of which Invited: 1 results) Remarks (8 results)

  • [Journal Article] DNA analysis for section identification of individual Pinus pollen grains from Belukha glacier, Altai Mountains, Russia2013

    • Author(s)
      Fumio Nakazawa, Jun Uetake, Yoshihisa Suyama, Ryo Kaneko, Nozomu Takeuchi, Koji Fujita, Hideaki Motoyama, Satoshi Imura, Hiroshi Kanda
    • Journal Title

      Environ. Res. Lett.

      Volume: 8 Issue: 1 Pages: 14-32

    • DOI

      10.1088/1748-9326/8/1/014032

    • Related Report
      2013 Research-status Report 2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 全ゲノム増幅法を用いた氷河中の花粉の同定2014

    • Author(s)
      中澤文男, 陶山佳久, 伊村智, 本山秀明
    • Organizer
      第5回極域科学シンポジウム
    • Place of Presentation
      国立極地研究所
    • Year and Date
      2014-12-02
    • Related Report
      2014 Annual Research Report
  • [Presentation] 全ゲノム増幅法を用いた氷河中の花粉1粒ずつの同定2014

    • Author(s)
      中澤文男, 陶山佳久, 伊村智, 本山秀明
    • Organizer
      日本地球惑星科学連合2014年大会
    • Place of Presentation
      パシフィコ横浜 会議センター
    • Year and Date
      2014-04-28
    • Related Report
      2014 Annual Research Report 2013 Research-status Report
  • [Presentation] 氷河中の花粉1粒ずつの全ゲノム増幅

    • Author(s)
      中澤文男, 陶山佳久
    • Organizer
      第4回極域科学シンポジウム
    • Place of Presentation
      国立極地研究所
    • Related Report
      2013 Research-status Report
  • [Presentation] Whole genome amplification法による氷河中の花粉一粒ずつのゲノムDNA増幅

    • Author(s)
      中澤文男, 陶山佳久
    • Organizer
      雪氷研究大会(2013・北見)
    • Place of Presentation
      北見工業大学
    • Related Report
      2013 Research-status Report
  • [Presentation] 氷河中のマツ属花粉1粒ずつのDNA分析により示された花粉長距離輸送の可能性

    • Author(s)
      中澤文男
    • Organizer
      第7回大気バイオエアロゾルシンポジウ
    • Place of Presentation
      滋賀県立大学(滋賀県彦根市)
    • Related Report
      2012 Research-status Report
  • [Presentation] DNA analysis for section identification of a pine pollen grain from the Belukha glacier, Altai Mountains, Russia

    • Author(s)
      Nakazawa, F., J. Uetake, Y. Suyama, R. Kaneko, N. Takeuchi, K. Fujita, H. Motoyama, S. Imura and H. Kanda
    • Organizer
      Third International Symposium on the Arctic Research
    • Place of Presentation
      日本科学未来館(東京都江東区)
    • Related Report
      2012 Research-status Report
  • [Presentation] ベルーハ氷河中のマツ属花粉1粒ずつのDNA分析によって明らかになった花粉の長距離輸送

    • Author(s)
      中澤文男, 陶山佳久, 竹内望, 藤田耕史, 伊村智, 本山秀明
    • Organizer
      第35回極域気水圏シンポジウム
    • Place of Presentation
      国立極地研究所(東京都立川市)
    • Related Report
      2012 Research-status Report
  • [Presentation] ベルーハ氷河中のマツ属花粉1粒ずつのDNA分析から導き出された花粉長距離輸送の可能性

    • Author(s)
      中澤文男, 陶山佳久, 竹内望, 藤田耕史, 伊村智, 本山秀明
    • Organizer
      雪氷研究大会(2012・福山)
    • Place of Presentation
      福山市立大学(広島県福山市)
    • Related Report
      2012 Research-status Report
  • [Presentation] DNA analysis for identification of a Pinus pollen grain at subsection level from the Belukha glacier, Altai Mountains, Russia

    • Author(s)
      Nakazawa, F., Y. Suyama, N. Takeuchi, K. Fujita, S. Imura, H. Kanda and H. Motoyama
    • Organizer
      13th International Palynological Congress and 9th International Organisation of Palaeobotany Conference - IPC/IOPC 2012
    • Place of Presentation
      中央大学(東京都文京区)
    • Related Report
      2012 Research-status Report
  • [Presentation] 氷河・氷床に含まれる花粉を利用した研究について

    • Author(s)
      中澤文男
    • Organizer
      日本第四紀学会2012年大会(埼玉) 公開シンポジウム「氷床コア等から得られる第四紀環境情報」
    • Place of Presentation
      立正大学(埼玉県熊谷市)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 亜節レベルでの同定を目的としたベルーハ氷河中のマツ属花粉1粒ずつのDNA分析

    • Author(s)
      中澤文男, 陶山佳久, 竹内望, 藤田耕史, 伊村智, 神田啓史, 本山秀明
    • Organizer
      日本地球惑星科学連合大会
    • Place of Presentation
      幕張メッセ国際会議場(千葉県千葉市)
    • Related Report
      2012 Research-status Report
  • [Remarks] researchmap

    • URL

      http://researchmap.jp/f_nakazawa/

    • Related Report
      2014 Annual Research Report
  • [Remarks] 氷河中の花粉DNA分析に関する論文の紹介記事

    • URL

      http://environmentalresearchweb.org/cws/article/news/53194

    • Related Report
      2014 Annual Research Report
  • [Remarks] Researchmap

    • URL

      http://researchmap.jp/f_nakazawa/

    • Related Report
      2013 Research-status Report
  • [Remarks] 氷河中の花粉DNA分析に関する論文の紹介記事

    • URL

      http://environmentalresearchweb.org/cws/article/news/53194

    • Related Report
      2013 Research-status Report
  • [Remarks] 氷河中の花粉DNA分析に関する論文

    • URL

      http://iopscience.iop.org/1748-9326/8/1/014032

    • Related Report
      2013 Research-status Report
  • [Remarks] Researchmap

    • URL

      http://researchmap.jp/f_nakazawa/

    • Related Report
      2012 Research-status Report
  • [Remarks] 氷河中の花粉DNA分析に関する論文の紹介記事

    • URL

      http://environmentalresearchweb.org/cws/article/news/53194

    • Related Report
      2012 Research-status Report
  • [Remarks] 氷河中の花粉DNA分析に関する論文

    • URL

      http://iopscience.iop.org/1748-9326/8/1/014032/

    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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